Insulin, human recombinant, zinc solution 12585014 from Thermo Fisher Scientific

Product Specs
ItemInsulin, human recombinant, zinc solution
CompanyThermo Fisher Scientific
Price $41.50Supplier Page View Company Product Page
Catalog Number12585014
Conjugate/TagUnconjugated
SpeciesHuman
Activity4 mg/mlPotency: (dry basis) >= 27 USP units/mg.Suggested range for supplementation: 2 to 20 µg/ml
Molecule NameInsulin
Source/Expression SystemRecombinant
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size4 mg/ml

Additional Product Information

Protein/Peptide:
Insulin

Protein/Peptide Species:
Human

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Do drugs with biliary toxicity cause cholangiocarcinoma?bioRxivApril 3, 2026Cai Zong, Kyumin Lim, Sierra A. Walker, Rui Dai, Mi Ho Jeong, Reanna George, Jung Hyun Jo, Shams Iqbal, Hyungsoon Im, Ralph WeisslederScientific), 0.025 mg/ml adenine (Cat. A8625, Sigma-Aldrich, St. Louis, MO, USA), 0.005 mg/ml insulin (Cat. 12585014, Gibco Invitrogen), 0.001 mg/ ml epinephrine (Cat. E4250, Sigma-Aldrich), 13.6 ng/ml 3,3′,5-Triiodo-L-thyronine

(2) OBESITY-INDUCED ENDOTHELIAL FENESTRATION AND CAPILLARY LEAKAGE CONTRIBUTE TO INCREASED PAIN SENSATIONbioRxivMarch 16, 2026Yuta Koui, Jonathan Richard Shin, Shuxuan Song, Christian A. Combs, Yoh-Suke MukouyamaGrowth Medium BulletKit (Lonza, 00192060), with or without 400 nM insulin (Thermo Fisher Scientific, 12585014), for 3–4 days. 3 days prior to insulin stimulation, either scramble control (Sigme, SIC001) or FOXO1

(3) Ribosome transfer via tunnelling nanotubes rescues protein synthesis in pancreatic cancer cellsCell Communication and SignalingMarch 7, 2026Stanislava Martínková, Denisa Jansová, Jana Vorel, Lucie Josefa Lamačová, Petr Daniel, Michael Hudec, Mário Boďo, Jan Hajer, Andrej Susor, Jan Trnkaprimary cells,InvivoGen,Ant-pm-1 "Insulin, human recombinant, zinc solution",Gibco™ Thermo Fisher,12585014 Human EGF Recombinant Protein,Gibco™ Thermo Fisher,PHG0311 "Hydrocortisone, 98%, Thermo Scientific

(4) Syndecan-1-targeted therapeutic antibody impairs macropinocytosis and elicits antitumor immunity in pancreatic cancerCell Reports MedicineFebruary 17, 2026Zecheng Yang, Madelaine S. Theardy, Shuaitong Chen, Yongkun Wei, Mitsunobu Takeda, Yue Zeng, Xiaofei Wang, Jun Yao, Jennifer Li, Prapassorn Thirasastr, et al.Hydrocortisone (Sigma, H0888), 100ng/mL Cholera Toxin (Sigma, C0852), and 10 μg/mL Insulin (Gibco, 12585014). 293FT cells were maintained in DMEM (high glucose) medium supplemented with 10% HI FBS. CHO-S cells

(5) Suppression of interferon signaling via small molecule modulation of TFAMeLifeFebruary 6, 2026Dionisia P Sideris, Hsuan Lee, Lyndsay Olson, Kalyan Nallaparaju, Keiichiro Okuyama, Jeffrey Ciavarri, Robert Lafyatis, Mads B Larsen, Bo Lin, Irene Alfaras, et al.RPMI-1640 Medium (Gibco, A10491-01) with 10% FBS (Gibco, 16140-071) with 0.2 Units/mL insulin (Gibco, 12585014). THP1-Dual cells were cultured in RPMI-1640 Medium (Gibco, A10491-01) with 10% FBS (Gibco, 16140-071)

(6) Oncogenic KRAS/ERK/JUNB signaling suppresses differentiation regulator GATA6 in pancreatic cancerJournal of Clinical InvestigationFebruary 2, 2026Zheng Zhong, Xinang Cao, Pei-Ju Liao, Raman Sethi, Jeffrey A. Klomp, Clint A. Stalnecker, Jinmiao Chen, Yue Wan, Channing J. Der, David M. Virshupcells were cultured in RPMI 1640 medium with 15% FBS and 4 μg/mL human recombinant insulin (Gibco, 12585014). All culture media contained 1 mM sodium pyruvate, 2 mM l-glutamine, and 1% (v/v) penicillin/streptomycin

(7) Robust Surfactant-Assisted One-Pot Sample Preparation for Label-Free Single-Cell and Nanoscale ProteomicsMethods in Molecular BiologyJanuary 29, 2026Zhangyang Xu, Rongge Zou, Nina C. Horn, Reta Birhanu Kitata, Tujin Shiessential medium,Thermo Fisher Scientific,21010046 human recombinant insulin,Thermo Fisher Scientific,12585014 fetal bovine serum,Thermo Fisher Scientific,16000044 penicillin/streptomycin,Thermo Fisher Scientific,15140148 Cell sorting

(8) Media Matters: Phenol Red and Fetal Bovine Serum Estrogen in Traditional Cell Culture Media Influence Human Mesenchymal Stromal Cell (hMSC) Processes and Differentiation in a Sex-Biased MannerbioRxivJanuary 16, 2026John C Bradford, Jennifer L RobinsonA17590.03), 500 uM Isobutylmethylxanthine (Thermo Scientific Chemicals # J64598.MC), 5 ug/ml Insulin (Gibco# 12585014), and 200 ug/ml of Ascorbic Acid (Sigma-Aldritch# 49752). Image analysis was performed utilizing specifically

(9) In vitro determination of patient-specific variation challenges the universal RBE gold standard for proton radiation therapyClinical and Translational Radiation OncologyJanuary 1, 2026Anna Kirstein, Dan Ionascu, Michael A.S. Lamba, Bianca M. Ruffolo, Katherine J. Crail, Anthony E. Mascia, Mathieu G. Sertorio, Benjamin H. Hinrichs, Chad Zender, Maria A. Lehn, et al.% adenine (#A2786, Sigma-Aldrich), 1 % cholera toxin (#227035, EMD Millipore), 1 % human insulin (#12585014, Invitrogen), 1 % hydrocortisone (#H0888, Sigma-Aldrich), 1 % penicillin–streptomycin, and 0.2 % amphotericin

(10) Targeting TRAF3IP2 disrupts cellular energetics through inhibition of NAMPT in triple negative breast cancerSci RepDecember 23, 2025Kurtis Willingham, Amin Izadpanah, Yasmine Rashad, Antonia Reilich, Fatemeh Daneshimehr, Steven Braun, Eckhard U. Alt, Reza Izadpanahnon-essential amino acid solution (cat# 11140050), sodium pyruvate (cat# 11360070), and insulin (cat# 12585014) acquired from Gibco, Inc. (Billings, MO). Adipose-derived stem cells (ASC) were isolated from adipose tissue

(11) Integrative CRISPR Screening and RNA Analyses Discover an Essential Role for PUF60 Interactions with 3' Splice Sites in Cancer ProgressionCancer ResearchDecember 18, 2025Alexandra T. Tankka, Yuhan Zhang, Jaclyn M. Einstein, Catherine J. Zhou, Vivian N. Pham, Jack T. Naritomi, Grady G. Nguyen, Amaya N. Mendez-Molina, Zhimin Hu, Orel Mizrahi, et al.(ThermoFisher Scientific Cat# 26140079) and 10 μg/ml recombinant human insulin (Fisher Scientific Cat# 12585014). SUM-149 (female, Applied Biological Materials Cat# T1250 RRID:CVCL_3422) immortalized cell lines were

(12) Protocol for retinal stress culture and single-cell analysisSTAR ProtocDecember 12, 2025Jackie L. Norrie, Cody Ramirez, Michael A. Dyer271120 DMEM/F12,Gibco,21041–025 FBS,Biowest,S1620 HEPES,Sigma,H0887 Insulin,Thermo Fisher Scientific,12585014 Penicillin-Streptomycin-Glutamine,Gibco,10378016 BSA,Sigma,A7906-50g Trizol,Invitrogen,15596026 Glycogen,Roche,10901393001 EtOH,Fisher,BP2818-4 Chloroform,Invitrogen,15593031 Isopropanol,Fisher,A416-2 Papain,Worthington,LS003119 L-Cysteine,Sigma,C7352-100G 0.5 M EDTA

(13) Intrinsic mechanotransduction during apical constriction licenses lineage competence in pluripotent stem cellsbioRxivDecember 3, 2025Mehdi Seif Hamouda, Celine Labouesse, George W. Wylde, Daniel Yamamoto, Yekaterina A. Miroshnikova, Srinjan Basu, Waterman M Waterman, Kevin J. Chalut12348017), along with Gibco B27 (0.5x, ThermoFisher 17504044), human insulin (12.5ug/mL, ThermoFisher 12585014), L-glutamine (0.78mM, or 2mM final with DMEM, ThermoFisher 25-030-081), Beta-Mercaptoethanol (100uM,

(14) A fast-progressing orthotopic ovarian cancer model reveals synergistic antitumor effects of AXL-targeting nanobodies and OlaparibGynecologic Oncology ReportsDecember 1, 2025Aarushi A. Caro, Alícia Gordún Peiró, Eva Hadadi, Yani Berckmans, Yvon Elkrim, Ayla Debraekeleer, Gisèle Mateu Cabrera, Kim De Veirman, An Coosemans, Damya Laouiheat-inactivated FCS (Capricorn Scientific, cat# FBS-12A), 12.8 µg mL−1 human recombinant insulin (Gibco, cat# 12585014), 100 units mL−1 penicillin, and 100 μg mL−1 streptomycin (Gibco, cat# 15140122). The mouse HM1 cell

(15) Sulindac sulfide suppresses oncogenic transformation through let-7b-mediated repression of K-Ras signalingCell Death DiscoveryNovember 14, 2025Zhipin Liang, Ruixia Ma, Bin Yi, Adam I. Riker, Yaguang XiDMEM/F12 medium (11330032, Thermo Fisher Scientific) supplemented with 5% FBS and 2 µg/ml insulin (12585014, Thermo Fisher Scientific). HCT116 cells were cultured in McCoy’s 5A medium (16600108, Thermo Fisher Scientific

(16) Zscan4 as a Candidate Conveyor of Early Developmental Defects in O-GlcNAc Transferase Intellectual DisabilityMolecular & Cellular ProteomicsNovember 1, 2025Veronica M. Pravata, Hao Jiang, Andrew T. Ferenbach, Angus Lamond, Daan M.F. van Aaltenalbumin fraction V (Thermo Fisher - 15260037), 12.5 μg/ml human recombinant insulin (Thermo Fisher - 12585014), 1 μM PD0325901 (Sigma - P2499), 3 μM CHIR99021 (Sigma - SML1046) and 100 units/ml LIF (produced in

(17) Low-GPX4 drives a sustained drug-tolerant persister state in TNBC by a targetable adaptive FSP1 upregulationRedox biologyNovember 1, 2025Chaudhary N, Mandal D, Choudhary BS, Patra S, Jain D, Poonia P, Shaikh S, Tekalkar S, Malvankar S, Shivashankar A, et al.the DMEM F-12 media supplemented with hEGF (20 ng/ml) (Sigma-E9644), Insulin (10μg/ml) (Invitrogen-12585014), cholera toxin (100 ng/ml) (catalogue no. C8052), hydrocortisone (1ug/ml) (catalogue no. H0888), penicillin/streptomycin

(18) Assessing the Independent and Combined Effects of Hyperosmolarity and Inflammatory Stress on Human Corneal Epithelial CellsInvest Ophthalmol Vis SciOctober 28, 2025Deeksha Prasad, Mohammad Gufran Siddiqui, Mahi Al Ahmad Dar, Rohini Sonar, Swati Singh, Pragnya Rao Donthineni, Sayan Basu, Vivek SinghR&D), 10 ng/mL human epidermal growth factor (EGF; PHG0311L; Thermo Fisher, USA), 5 µg/mL insulin (12585014; Thermo Fisher, USA), and 1% antibiotic–antimycotic solution (15240062; Thermo Fisher, USA). Prior to

(19) FTO depletion does not alter m 6 A stoichiometry in AML mRNA: a reassessment using direct RNA nanopore sequencingbioRxivOctober 27, 2025Luke S. Nicholson, Catarina Guimarães-Teixeira, Jianheng Fox Liu, Hui Xian Poh, Samie R. Jaffrey1 mM sodium pyruvate (Life Technologies, 11360070), and 10 μg/ml human insulin (Life Technologies, 12585014). All media were supplemented with 10% fetal bovine serum (FBS) and 1X penicillin-streptomycin (Gibco,

(20) Precision Oncology for High-Grade Gliomas: A Tumor Organoid Model for Adjuvant Treatment SelectionBioengineering (Basel)October 19, 2025Arushi Tripathy, Sunjong Ji, Habib Serhan, Reka Chakravarthy Raghunathan, Safiulla Syed, Visweswaran Ravijumar, Sunita Shankar, Dah-Luen Huang, Yazen Alomary, Yacoub Haydin, et al.without vitamin, (Gibco 12587010), 5 mL Non-Essential Amino Acids (Gibco 11140050), 400 μL insulin (Gibco 12585014), and 500 μL 2-mercaptoethanol (Gibco 21985023)) on a rotary shaker in an incubator at 37 degrees Celsius

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Insulin, human recombinant, zinc solution from Thermo Fisher Scientific

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Thermo Fisher Scientific

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Insulin, human recombinant, zinc solution from Thermo Fisher Scientific

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