Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate ES007 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate
CompanyR&D Systems, a Bio-Techne Brand
Price $337.00Supplier Page View Company Product Page
Catalog NumberES007
Size1 mg
ApplicationsBioactivity
Purity>95%, by HPLC
Molecular Weight1145.2 Da
SpeciesMulti-Species
Molecule NameProtease Substrates
StorageSamples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Angiotensin-(1–7) modulates gut-bone marrow axis in diabetesBiochemical PharmacologyNovember 1, 2025Kishore Chittimalli, Ishan Manandhar, Stephen Adkins, Manseb Rahman, Andrew Toelle, Ramakumar Tummala, Bina Joe, Yagna P.R. Jarajaputhe colon tissue lysates was carried out by using enzyme-specific fluorogenic substrates (ES005 and ES007, R&D Systems, Minneapolis MN), as described before.27 Enzyme-specific inhibitors, captopril or MLN-4760,

(2) Potential Beneficial Role of Nitric Oxide in SARS-CoV-2 Infection: Beyond Spike-Binding InhibitionAntioxidants (Basel)October 26, 2024Sergio Sánchez-García, Antonio Castrillo, Lisardo Boscá, Patricia Prietopatients. 2. Materials and Methods 2.1. Materials The ACE2 substrate Mca-ACE2 (Mca-YVADAPK(Dnp)-OH) (#ES007) was from Bio-Techne (Minneapolis, MN, USA). The ACE2 inhibitor MLN-4760 (2(S)-(1(S)-carboxy-2-(3-(3,5-dichlorobenzyl)-3H-imidazol-4-yl)-ethylamino)-4-methylpentanoic

(3) Discovery, validation, and prodrug design of an ACE2 activator for treating bacterial infection-induced lung inflammationJournal of Controlled ReleaseDecember 1, 2023Peng Lu, Faith Leslie, Han Wang, Anjali Sodhi, Chang-yong Choi, Andrew Pekosz, Honggang Cui, Hongpeng Jiadetermined by measuring the fluorescence intensity of ACE2 substrate Mca-YVADAPK(Dnp)-OH (Catalog no. ES007; R&D Systems) [15]. In brief, samples were diluted in assay buffer (50 mM MES, 300 mM NaCl, 10 nM ZnCl2,

(4) Antrodia cinnamomea May Interfere with the Interaction Between ACE2 and SARS-CoV-2 Spike Protein in vitro and Reduces Lung Inflammation in a Hamster Model of COVID-19Journal of Inflammation ResearchOctober 26, 2023Lan-Hui Li, Hsiao-Wen Chiu, Wei-Ting Wong, Ko-Chieh Huang, Tzu-Wen Lin, Shui-Tein Chen, Kuo-Feng HuaRecombinant proteins for human ACE-2 (933-ZN) and the Mca-YVADAPK(Dnp)-OH fluorogenic peptide substrate (ES007) were sourced from R&D Systems (Minneapolis, MN). We utilized the Bright-Glo Luciferase Assay System,

(5) A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19BiosensorsJune 27, 2023Suresh V, Sheik DA, Detomasi TC, Zhao T, Zepeda T, Saladi S, Rajesh UC, Byers K, Craik CS, Davisson VJ.angiotensin-converting enzyme 2 (933-ZN-10), and Mca-YVADAPK(Dnp)-OH fluorogenic peptide substrate (ES007) were purchased from R&D Systems (Minneapolis, MN, USA). Artificial saliva was purchased from Biochemazone

(6) Optimized ACE2 decoys neutralize antibody-resistant SARS-CoV-2 variants through functional receptor mimicry and treat infection in vivoScience advancesDecember 9, 2022Torchia JA, Tavares AH, Carstensen LS, Chen DY, Huang J, Xiao T, Mukherjee S, Reeves PM, Tu H, Sluder AE, et al.ACE2-Fc proteins, a fluorogenic substrate was used. ACE2 cleaves the Mca-YVADAPK(Dnp)-OH (R&D Systems; ES007) peptide at the C-terminal lysine residue. Cleavage liberates the Dnp (2,4-dinitrophenyl) moiety that

(7) Anti-human ACE2 antibody neutralizes and inhibits virus production of SARS-CoV-2 variants of concerniScienceSeptember 16, 2022Chaouat AE, Brizic I, Kucan Brlic P, Atari N, Kliker L, Alfi O, Mandelboim M, Wolf D, Tafish L, Kol I, et al.peptides, and recombinant proteins" ,, Mca-Y-V-A-D-A-P-K(Dnp)-OH fluorogenic peptide,R&D Systems,Cat#ES007 o-phenylenediamine dihydrochloride,Sigma,Cat#P8412 RBD-Ig,"Chaouat et al., (2021)",N/A ACE2-Ig,"Chaouat

(8) Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognitionNature communicationsApril 5, 2022Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, et al.recommendation77. Enzymatic assay The fluorogenic peptide substrate Mca-Y-V-A-D-A-P-K(Dnp)-OH (R&D Systems, Catalog # ES007) was used to measure the activity of PreP. The reaction was monitored on a Synergy Neo microplate reader

(9) Sex- and age-specific regulation of ACE2: Insights into severe COVID-19 susceptibilityJournal of Molecular and Cellular CardiologyMarch 1, 2022Anissa Viveiros, Mahmoud Gheblawi, Preetinder K. Aujla, Deanna K. Sosnowski, John M. Seubert, Zamaneh Kassiri, Gavin Y. Ouditabsence of ACE2 inhibitor DX-600 (Cayman Chemical) for thirty minutes. Fluorogenic Peptide Substrate VI (ES007, R&D Systems) was subsequently added (final concentration of 50 μM in Assay buffer), and kinetic measurements

(10) The collectrin-like part of the SARS-CoV-1 and -2 receptor ACE2 is shed by the metalloproteinases ADAM10 and ADAM17FASEB journal : official publication of the Federation of American Societies for Experimental BiologyMarch 1, 2022Niehues RV, Wozniak J, Wiersch F, Lilienthal E, Tacken N, Schumertl T, Garbers C, Ludwig A, Düsterhöft S.Triton X‐100; adjust pH6.5) and 5 µl 0.1 mM fluorogenic ACE2 peptide substrate (Mca‐YVADAPK(Dnp)‐OH; ES007, R&D systems, Minneapolis, Minnesota, USA) in DMSO. 50 µl supernatant or 5 µl cell lysate were mixed

(11) Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by epistasis acting on ACE2PLoS biologyDecember 1, 2021Castiglione GM, Zhou L, Xu Z, Neiman Z, Hung CF, Duh EJ.

(12) SARS-CoV-2 spike protein binding selectively accelerates substrate-specific catalytic activity of ACE2Journal of biochemistryOctober 11, 2021Kiseleva AA, Troisi EM, Hensley SE, Kohli RM, Epstein JA.pseudosubstrate were obtained from R&D systems (cat# 933-ZN, 10549-CV, 10500-CV-100, 10558-CV-100 and ES007, respectively, R&D Systems Inc., MN, USA). The Caspase-1 pseudosubstrate contained an N-terminal 7-methoxycoumarin

(13) Molecular Masks for ACE2 to Effectively and Safely Block SARS-CoV-2 Virus EntryInternational journal of molecular sciencesAugust 20, 2021Shukla SP, Cho KB, Rustagi V, Gao X, Fu X, Zhang SX, Guo B, Udugamasooriya DG.10 µM), and Mca-Y-V-A-D-A-P-K (Dnp)-OH, Fluorogenic Peptide Substrate VI from R&D System, Inc. (Cat# ES007, Minneapolis, MN, USA). The reaction buffer was prepared using 1M NaCl, 0.5 mM ZnCl2, 75 mM Tris, protease

(14) ACE2/ACE imbalance and impaired vasoreparative functions of stem/progenitor cells in agingGeroScienceJune 1, 2021Joshi S, Chittimalli K, Jahan J, Vasam G, Jarajapu YP.lysates or plasma and cell supernatants by using enzyme-specific fluorogenic substrates (ES005 and ES007 for ACE and ACE2, respectively, R&D Systems), as reported before [31]. Enzyme-specific inhibitors, captopril

(15) ACE2 gene transfer ameliorates vasoreparative dysfunction in CD34+ cells derived from diabetic older adultsClinical science (London, England : 1979)January 29, 2021Joshi S, Montes de Oca I, Maghrabi A, Lopez-Yang C, Quiroz-Olvera J, Garcia CA, Jarajapu YPR.lysates or plasma and cell supernatants by using enzyme-specific fluorogenic substrates (ES005 and ES007 for ACE and ACE2, respectively, R&D Systems), as reported before [25]. Enzyme-specific inhibitors, captopril

(16) Humanized COVID-19 decoy antibody effectively blocks viral entry and prevents SARS-CoV-2 infectionEMBO molecular medicineJanuary 11, 2021Huang KY, Lin MS, Kuo TC, Chen CL, Lin CC, Chou YC, Chao TL, Pang YH, Kao HC, Huang RS, et al.

(17) High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activityThe Journal of biological chemistryDecember 25, 2020Lu J, Sun PD.Sinobiological, Inc. Fluorogenic peptide substrates were obtained from R&D systems, where Mca-YVADAPK(Dnp)-OH (ES007) was used as an angiotensin II like substrate and Mca-RPPGFSAFK(Dnp)-OH(ES005) was a fluorogenic peptide

(18) High affinity binding of SARS-CoV-2 spike protein enhances ACE2 carboxypeptidase activitybioRxiv : the preprint server for biologyJuly 1, 2020Lu J, Sun PD.Sinobiological, Inc. Fluorogenic peptide substrates were obtained from R&D systems, where Mca-YVADAPK(Dnp)-OH (ES007) was used as an angiotensin II like substrate and Mca-RPPGFSAFK(Dnp)-OH(ES005) was a fluorogenic peptide

(19) A Dynamic Variation of Pulmonary ACE2 Is Required to Modulate Neutrophilic Inflammation in Response to Pseudomonas aeruginosa Lung Infection in MiceThe Journal of ImmunologyDecember 1, 2019Chhinder P. Sodhi et al.measuring the fluorescence intensity of ACE2 substrate Mca-Y-V-A-D-A-P-K(Dnp)-OH (R&D Systems, Cat# ES007) (34). In brief, samples were diluted in assay buffer (50 mM MES, 300 mM NaCl, 10 nM ZnCl2, and 0.01%

(20) Hypoxic regulation of angiotensin-converting enzyme 2 and Mas receptor in human CD34(+) cellsJournal of cellular physiologyNovember 1, 2019Joshi S, Wollenzien H, Leclerc E, Jarajapu YP.cell-lysates and cell-supernatants by using enzyme-specific fluorogenic substrates (ES005 for ACE and ES007 for ACE2; R&D Systems), respectively, as described before. (Joshi, Balasubramanian, Vasam, & Jarajapu,

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Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate from R&D Systems, a Bio-Techne Brand

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