IL-4 Protein 404-ML-010 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-4 Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $257.00Supplier Page View Company Product Page
Catalog Number404-ML-010
SpeciesMouse
Molecule NameIL-4
Size10 ug (also 25 ug, 50 ug, 100 ug)
Source/Expression SystemE. coli-derived Recombinant Mouse IL-4 Protein
NCBI Full Gene Nameinterleukin 4
NCBI Gene AliasesBSF-1, IL-4, BCGF1, Il-4, BCGF-1, BSF1
FormatLyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein
Purity>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
ApplicationsBioactivity
Conjugate/TagUnconjugated
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Sizes Available: 10 ug (also 25 ug, 50 ug, 100 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Immune-induced TCR-like antibodies regulate specific T cell response in miceNat CommunApril 16, 2026Kazuki Kishida, Keisuke Kawakami, Hiroaki Tanabe, Wataru Nakai, Koji Yonekura, Shigeyuki Yokoyama, Hisashi Arasecell isolation kit (130-090-862, Miltenyi Biotec). These B cells were co-cultured with 10ng/ml IL-4 (404-ML-010, R&D Systems), 5 μg/ml anti-CD40 antibody (102812, Biolegend), 5 μg/ml anti-IgM antibody (115-006-075,

(2) Mutant ribosomal protein RPS15 drives B cell malignancy through oxidative stress and genomic instabilityNature CommunicationsMarch 30, 2026Catherine Gutierrez, Marwan Kwok, Neil Ruthen, Peyton Waddicor, Christina Curran, Tamara Ouspenskaia, Doris Fu, Brendan Smalec, Samantha Sedor, Binyamin A. Knisbacher, et al.cultured in RPMI/10% FBS containing LPS (50 μg/mL; Sigma, #L6511), murine IL-4 (10 ng/mL; R&D Systems, #404ML010) and β-mercaptoethanol (1:500,000; ThermoFischer Scientific, #21985023) prior to downstream functional

(3) Allogeneic MHC-mismatched microglia-like cell replacement as a therapeutic approach for multiple sclerosisJournal of NeuroinflammationJanuary 8, 2026Irene Benito-Cuesta, Jin-Hong Min, Yuxi Guo, Giulia Adriana Virgilio, Valerie Suerth, Stefan Bencina, Paula Trigo-Alonso, Keying Zhu, Shin-Yu Kung, Majid Pahlevan Kakhki, et al.proinflammatory phenotype (M1), or (ii) 20ng/ml IL-10 (R&D systems, 417-ML-005), 20ng/ml IL-4 (R&D systems, 404-ML-010) and 20ng/ml TGF-b 1 (R&D systems, 7666-MB-005) to promote an anti-inflammatory phenotype (M2). Unstimulated

(4) CCL5 paradoxically regulates glomerular injury by skewing macrophage polarizationJCI insightNovember 10, 2025Kadariswantiningsih IN, Okunaga I, Yamasaki K, Empitu MA, Yamada H, Makino SI, Hotta A, Yagita H, Aizawa M, Koyama-Nasu R, et al.

(5) Identification of UVB-induced TREM-1–positive tolerogenic dendritic cells and TREM-1 signaling as a target in the prevention of UVB-induced immune suppression and skin carcinogenesisJournal of Investigative DermatologyNovember 8, 2025Carlos Alberto Mier-Aguilar, Mohammad Asif Sherwani, Yuko Tsuruta, Noha Abdelsamad, David K. Crossman, Sejong Bae, Andrzej T. Slominski, Craig A. Elmets, Nabiha Yusuf, Hui Xuantibiotic-antimycotic, 20 ng/ml of GM-CSF (R&D Systems, cat: 415-ML-010), and 10 ng/ml of IL-4 (R&D Systems, cat: 404-ML-010) in non-Tissue culture treated plates. We added 10 mL of the media on day 3 and collected floating cells

(6) CTRP6 as a negative regulator of anti-inflammatory M2 macrophage polarizationImmunometabolism (Cobham)October 27, 2025Jeevotham Senthil Kumar, Emma Kempton, Muhammad Zubair Mehboob, Dingbo Lin, Xia LeiMA, USA) and 20 ng/mL IFNγ (485-MI-100, R&D Systems, Minneapolis, MN, USA) for M1, and 20 ng/mL IL-4 (404-ML-010, R&D Systems, Minneapolis, MN, USA) for M2. Naïve macrophages (M0) were cultured in medium containing

(7) CTRP6 as a negative regulator of anti-inflammatory M2 macrophage polarizationImmunometabolism (Cobham)October 27, 2025Jeevotham Senthil Kumar, Emma Kempton, Muhammad Zubair Mehboob, Dingbo Lin, Xia LeiMA, USA) and 20 ng/mL IFNγ (485-MI-100, R&D Systems, Minneapolis, MN, USA) for M1, and 20 ng/mL IL-4 (404-ML-010, R&D Systems, Minneapolis, MN, USA) for M2. Naïve macrophages (M0) were cultured in medium containing

(8) Chronic intermittent hypoxia drives M1 macrophage polarization in dorsal root gangliaBrain, Behavior, and ImmunityOctober 1, 2025Samuel B. Chivers, Mary Ann Andrade, Cassandra L. McLay, Kristopher L. Wieland, Pankil K. Shah, Glenn M. Toney, Nathaniel A. Jeske(LPS from Escherichia coli O55:B5, Cayman Chemical 19660) for M1 polarization, or 20 ng/mL IL-4 (BioTechne, 404-ML-010) for M2 polarization. Incubated for 18 h prior to experimental testing. 2.9. Western blotting DRG

(9) Leukocyte-type 12/15-lipoxygenase is essential for timely inflammation-resolution and effective tissue regeneration following skeletal muscle injuryMolecular metabolismOctober 1, 2025Sharma B, Lu X, Rehman H, Figueiredo VC, Davis C, Van Remmen H, Kuang S, Brooks SV, Maddipati KR, Markworth JF.

(10) Inflammatory arthritis immune related adverse events represent a unique autoimmune disease entity primarily driven by T cells, but likely not autoantibodiesmedRxiv : the preprint server for health sciencesSeptember 26, 2025Zhu X, Yu Y, Li Y, Wang P, Li Y, McCabe C, Chen S, Langenfeld HE, Hanson AC, Sharp BE, et al.

(11) ALS/FTD-linked TBK1 deficiency in microglia induces an aged-like microglial signature and drives social recognition deficits in miceNature communicationsAugust 26, 2025Lenoel I, Ribon M, Lorenc F, Diebold A, Philibert CE, Robaldo D, Badsi M, Perronnet J, Lameth J, Berriat F, et al.

(12) Inflammatory cytokine interleukin-18 prevents murine miscarriage by inducing appropriate inflammationScientific reportsAugust 21, 2025Horii Y, Ino H, Sasaki F, Ishibashi M, Koike E, Kawamoto Y, Flavell RA, Ohashi R, Suzuki S, Negishi Y, et al.

(13) The role of tungsten in modulating DNA double-strand break repair and class switch recombinationToxicology and applied pharmacologyJuly 1, 2025Bakadlag R, Chou H, Guilbert C, Luo VM, Orthwein A, Mann KK.

(14) The ubiquitin E3 ligase TRIM21 suppresses type I interferon signaling via STING degradation and ameliorates systemic autoimmunityExperimental & molecular medicineJuly 1, 2025Kim DS, Park Y, Tsokos GC, Cho ML, Kwok SK.

(15) Thymic dendritic cell-derived IL-27p28 promotes the establishment of functional bias against IFN-γ production in newly generated CD4(+) T cells through STAT1-related epigenetic mechanismseLifeMay 14, 2025Zhang J, Tang H, Wu H, Pang X, Jin R, Zhang Y.

(16) Differential Roles of Macrophages and Microglia in Subretinal Fibrosis Secondary to Neovascular Age-Related Macular DegenerationInvestigative ophthalmology & visual scienceMarch 3, 2025Szczepan M, Llorián-Salvador M, Yi C, Hughes D, Mack M, Chen M, Xu H.

(17) WNT11 Promotes immune evasion and resistance to Anti-PD-1 therapy in liver metastasisNature communicationsFebruary 7, 2025Jiang W, Guan B, Sun H, Mi Y, Cai S, Wan R, Li X, Lian P, Li D, Zhao S.replaced. On day 7, M2 macrophages were stimulated with IL-4 (10 ng/ml, R&D Systems, Inc., cat. no. 404-ML-010) for 2 days, while M1 macrophages were stimulated using LPS (10 ng/ml, Beyotime Institute of Biotechnology,

(18) Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responsesCell researchFebruary 1, 2025Zhao Q, Wang Q, Yao Q, Yang Z, Li W, Cheng X, Wen Y, Chen R, Xu J, Wang X, et al.

(19) Macrophage to neuron communication via extracellular vesicles in neuropathic pain conditionsHeliyonJanuary 15, 2025Picco F, Zeboudj L, Oggero S, Prato V, Burgoyne T, Gamper N, Malcangio M.

(20) miR-210 promotes the anti-inflammatory phenotype and M2 polarization in murine macrophagesFrontiers in immunologyJanuary 1, 2025Neculachi CA, Nastase-Rusu EG, Cherry L, Marinescu-Colan CI, Tastsoglou S, Cosman BP, Popa AM, Panciuc C, Zaccagnini G, Catrina SB, et al.anti-inflammatory phenotype was induced by incubating the cells with 20 ng/mL IL-4 (R&D Systems, #RD-404-ML-010) for 72 hours. Control wells were maintained under identical conditions without IL-4 treatment. 2.6

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IL-4 Protein

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IL-4 Protein

IL-4 Protein

IL-4 Protein from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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IL-4 Protein from R&D Systems, a Bio-Techne Brand

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