Fig 1: Secreted proteins that are downregulated in AGRP neurons with food-deprivation reduce food intake.(A–F) Double smFISH for Agrp and (A) Bmp3 or (D) Cbln4. Scale, 10 µm. Population counts (B, E) and cumulative probability distributions (C, F) for Bmp3 and Cbln4 (p = 9e-12 and p = 6.8e-17, ks-test). Bmp3 fed, n = 142; Bmp3 FD, n = 157 cells; Cbln4 fed, n = 145; Cbln4 FD, n = 132; 3 mice per condition. (G) Mean food intake (24 hr) after intracerebroventricular injection of either saline or recombinant BMP3 (ANOVA, F2,24 = 7.5, p = 0.003), CBLN4 (ANOVA, F2,24 = 8.0, p = 0.002), ENPP2 (ANOVA, F2,27 = 7.3, p = 0.003), or Pleiotrophin (unpaired t-test). Holm-Sidak correction for multiple comparisons. Data is mean ± s.e.m. n.s., p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.DOI: http://dx.doi.org/10.7554/eLife.09800.015