Fig 1: Bulk RNA-seq of type I IFN-stimulated PBMCs.(A, B) Volcano plots of differentially expressed transcripts mapping to protein-coding (A) and non-coding (B) genes in PBMCs stimulated with 250 U/mL of the indicated type I IFNs for 24 h, compared to mock-stimulated PBMCs (n = 3 matched biological replicates). Statistical significance was assessed using a two-sided Wald test (DESeq2). Red and blue indicate transcripts significantly up-regulated or down-regulated (Benjamini-Hochberg adjusted p-value ≤ 0.05; fold change ≥ 1.5), respectively. The total number of differentially expressed transcripts (DETs) are shown. (C) Heatmap of all transcripts mapping to protein-coding genes that are differentially expressed in response to at least one type I IFN (4585 up- and 3343 down-regulated). (D, E) Heatmaps showing expression of coding DETs mapping to annotated ISGs (D) and the top 50 upregulated ISG-derived transcripts (E). Transcript names follow Ensembl nomenclature. For transcripts belonging to technically indistinguishable groups, shared prefixes are followed by unique suffixes in brackets, separated by slashes (e.g., RNA[201/207]); for groups with more than five indistinguishable RNAs, the total number is indicated (e.g., RNA[N = 35]). In (C–E), transcripts are ranked by Z-score across all type I IFN-stimulated samples. Data are from one experiment with three donors. See also Appendix Figs. S7–S13 and Datasets EV9–EV13.
Fig 2: Phosphorylation of STAT proteins in response to IFN-α2a.(A) Overview of the type I IFN system. (B) Schematic representation of the mass cytometry workflow to quantify protein phosphorylation in response to type I IFN-treatment. (C) Median expression of pSTAT1, pSTAT3, pSTAT4, pSTAT5 and pSTAT6 in PBMCs treated with increasing concentrations of IFN-α2a for 15 min. (D) Depiction of the data shown in (C) as a heatmap. (E) Violin plots showing expression of each pSTAT in the indicated cell types in response to increasing concentrations of IFN-α2a. The number of cells analysed is shown in parentheses. Violin plots for all cell types are shown in Appendix Fig. S1C. Data are from one experiment with one donor. See also Appendix Figs. S1 and S2 and Datasets EV1 and EV2.
Supplier Page from PBL Assay Science for Human Interferon Alpha D (Alpha 1)
Protein/Peptide:IFN Alpha D (Alpha 1 (Val 114))
Protein/Peptide Species:Human