Fig 1: Screening and identification of vascular-endothelial-cell-specific factors in CRC from single-cell databases(A) t-SNE plot revealing 10 major cell clusters from dataset GEO: GSE144735, colored by cell type.(B) t-SNE plots showing normalized expression of two representative endothelial cell marker genes in GEO: GSE144735.(C) Heatmap of differentially expressed genes between normal and CRC vascular endothelial cells in GEO: GSE144735. See also Table S2.(D) Volcano plot of differential gene expression between normal and CRC vascular endothelial cells in GEO: GSE144735. See also Table S2.(E) Intersection of differentially expressed genes from datasets GEO: GSE144735, GSE132465 in vascular endothelial cells from normal and CRC tissues.(F) Immunofluorescence staining of CD31 and IGFBP5 in paired normal and CRC tissues. Scale bars: 100 μm.(G) Immunofluorescence staining of CD31 and IGFBP5 in blood vessels from paired normal and CRC tissues. Scale bars: 50 μm.