Recombinant Human/Mouse Wnt-5a Protein, CF 645-WN-010/CF from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Human/Mouse Wnt-5a Protein, CF
CompanyR&D Systems, a Bio-Techne Brand
Price $716.00Supplier Page View Company Product Page
Catalog Number645-WN-010/CF
Size10 ug
ApplicationsBioactivity
FormatLyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS
Purity>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Molecular WeightObserved: 45 kDa, reducing conditions
Theoretical: 38 kDa
Conjugate/TagUnconjugated
SpeciesHuman, Mouse
Molecule NameWnt-5a
Source/Expression SystemCHO-derived Recombinant Human/Mouse Wnt-5a Protein, CF
NCBI Full Gene Namewingless-type MMTV integration site family, member 5A, Wnt family member 5A
NCBI Gene AliaseshWNT5A, 8030457G12Rik, Wnt-5a
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution

Add to Compare List

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) ROME, an Ancient Gene with a Novel Function in Vertebrates, Is a Key Modulator of Embryonal Development and Cancer MetastasisCancer Res CommunMarch 1, 2026Anna Molotkova, Emre Deniz, Matthew Swift, Eric Glasgow, Junfeng Ma, Jeffrey A. Petro, Isabel Frye, Metin Ozdemirli, Dusan Pesic, Gerard P. Ahern, et al.(pRL-SV40) in a 10:1 ratio. On the next day, cells were stimulated with recombinant Wnt3a (R&D Systems, #645-WN-010/CF) for 4.5 hours, and then luciferase assay was conducted with the Dual Luciferase Reporter Assay System

(2) ROME, an ancient gene with a novel function in vertebrates, is a key modulator of embryonal development and cancer metastasisCancer Research CommunicationsMarch 1, 2026Anna Molotkova, Emre Deniz, Matthew Swift, Eric Glasgow, Junfeng Ma, Jeffrey A. Petro, Isabel Frye, Metin Ozdemirli, Dusan Pesic, Gerard P. Ahern, et al.(pRL-SV40) in a 10:1 ratio. On the next day, cells were stimulated with recombinant Wnt3a (R&D Systems, #645-WN-010/CF) for 4.5 hours, and then luciferase assay was conducted with the Dual Luciferase Reporter Assay System

(3) Disruption of bile acid homeostasis potentiates Paneth cell ablation by activating the intestinal Farnesoid X receptor in necrotizing enterocolitisnpj Biofilms and MicrobiomesJanuary 8, 2026Jing Xiong, Ting Guo, Tingting Gao, Yeerfan Aierken, Qingqi Chong, Zhibao Lv, Li Lueach well by pipetting the medium gently down the wall of the well. For experiments involving Wnt5a (645-WN-010/CF, R&D Systems) ligands, the media was made by supplementing ENR media with 100 ng/ml Wnt5a. Finally,

(4) Shank3 related oligodendrocyte alterations in autism are restored by Erk pathway inhibitionMolecular PsychiatryNovember 7, 2025Yuhua Ma, Helen Friedericke Bauer, Juergen Bockmann, Michael Schön, Tobias M. Boeckers, Anne-Kathrin LutzWnt3a/5a treatment in vitro For in vitro Wnt3a (Wnt3a: 1324-WN-010/CF, R&D systems) and Wnt5a (Wnt5a: 645-WN-010/CF, R&D systems) treatment, at DIV 18 medium was replaced by a mix of fresh oligodendrocytes differentiation

(5) WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signalingNature communicationsMay 24, 2025Voss JH, Koszegi Z, Yan Y, Shorter E, Grätz L, Lanner JT, Calebiro D, Schulte G.from Biotechne with the following catalog numbers: human WNT-3A (5036-WN-010), human/mouse WNT-5A (645-WN-010/CF), recombinant human WNT-10B (7196-WN-010), human WNT-16B (7790-WN-025), R-spondin 1 (4645-RS-025). To

(6) Frizzled5 controls murine intestinal epithelial cell plasticity through organization of chromatin accessibilityDevelopmental cellNovember 15, 2024Deng L, He XC, Chen S, Zhang N, Deng F, Scott A, He Y, Tsuchiya D, Smith SE, Epp M, et al.Recombinant Mouse Wnt-3a Protein,R&D Systems,Cat#1324-WN-010/CF Recombinant Human/Mouse Wnt-5a Protein,R&D Systems,Cat#645-WN-010/CF Y-27632 dihydrochloride,MilliporeSigma,Cat#Y0503 Opal 520 Reagent Pack,AKOYA,Cat#FP1487001KT Opal

(7) Class-Wide Analysis of Frizzled-Dishevelled Interactions Using BRET Biosensors Reveals Functional Differences among Receptor ParalogsACS SensorsSeptember 27, 2024Lukas Grätz, Jan H. Voss, Gunnar Schultewere found to be negative. Ligands Recombinant human WNT-3A (#5036-WN-010), human/mouse WNT-5A (#645-WN-010/CF), human WNT-5B (#7347-WN-025), human WNT-16B (#7790-WN-025), and human Norrin (#3014-NR-025) were purchased

(8) Structural insights into Frizzled3 through nanobody modulatorsNature communicationsAugust 22, 2024Hillier J, Zhao Y, Carrique L, Malinauskas T, Ruza RR, Chang TH, Yi G, Duyvesteyn HME, Yu J, Lu W, et al.nanobodies, DEP (produced by bacterial expression) or Wnt5a (purchased from Biotechne R&D systems, cat# 645-WN-010/CF), and biotinylated FZD3/6 CRD, or multidomain FZD3 (produced by in vivo biotinylation in HEK293T cells

(9) Wnt5a Promotes Axon Elongation in Coordination with the Wnt-Planar Cell Polarity PathwayCellsJuly 28, 2024Ahmad S, Attisano L.were treated with the conditioned media (CM) or recombinant Wnt5a (R&D Systems, Minneapolis, MN, USA, 645-WN-010/CF) 4 h after plating. In the transfection experiments, the media was replaced with a fresh one 4 h after

(10) Single-cell RNA sequencing of intestinal crypts reveals vital events in damage repair and the double-edged sword effect of the Wnt3/β-catenin pathway in irradiated miceRedox biologyDecember 1, 2023Yuan T, Zhang J, Zhao Y, Guo Y, Fan S.culture, 4 ng/mL WNT3A protein (R&D System, Cat.no 1324-WN-002/CF), 2 ng/mL WNT4 (R&D System, Cat.no 645-WN-010/CF), 2 ng/mL WNT5 protein (Abnova, Cat.no H00054361–P01), 200 ng/mL MIF protein (MCE, Cat.no HY-P7388),

(11) A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neuronsSTAR protocolsSeptember 15, 2023Nishimura K, Ásgrímsdóttir ES, Yang S, Arenas E.fibroblast growth factor8B (FGF8B),PeproTech,Cat#100-25-100 Recombinant human WNT5A,R&D Systems,Cat#645-WN-010/CF Recombinant human brain-derived neurotrophic factor (BDNF),R&D Systems,Cat#248-BD-025/CF Recombinant

(12) WNT5a Signaling through ROR2 Activates the Hippo Pathway to Suppress YAP1 Activity and Tumor GrowthCancer researchApril 4, 2023Wang K, Ma F, Arai S, Wang Y, Varkaris A, Poluben L, Voznesensky O, Xie F, Zhang X, Yuan X, et al.

(13) Convergent deployment of ancestral functions during the evolution of mammalian flight membranesScience advancesMarch 24, 2023Feigin CY, Moreno JA, Ramos R, Mereby SA, Alivisatos A, Wang W, van Amerongen R, Camacho J, Rasweiler JJ 4th, Behringer RR, et al.

(14) Ehrlichia Wnt short linear motif ligand mimetic deactivates the Hippo pathway to engage the anti-apoptotic Yap-GLUT1-BCL-xL axisbioRxiv : the preprint server for biologyMarch 7, 2023Byerly CD, Patterson LL, Pittner NA, Solomon RN, Patel JG, Rogan MR, McBride JW.

(15) A DLG1-ARHGAP31-CDC42 axis is essential for the intestinal stem cell response to fluctuating niche Wnt signalingCell stem cellFebruary 2, 2023Castillo-Azofeifa D, Wald T, Reyes EA, Gallagher A, Schanin J, Vlachos S, Lamarche-Vane N, Bomidi C, Blutt S, Estes MK, et al.

(16) Pharmacological clearance of senescent cells improves cardiac remodeling and function after myocardial infarction in female aged miceMechanisms of ageing and developmentDecember 1, 2022Salerno N, Marino F, Scalise M, Salerno L, Molinaro C, Filardo A, Chiefalo A, Panuccio G, De Angelis A, Urbanek K, et al.

(17) WNT5A-RHOA Signaling Is a Driver of Tumorigenesis and Represents a Therapeutically Actionable Vulnerability in Small Cell Lung CancerCancer researchNovember 15, 2022Kim KB, Kim DW, Kim Y, Tang J, Kirk N, Gan Y, Kim B, Fang B, Park JL, Zheng Y, et al.

(18) Human PSCs determine the competency of cerebral organoid differentiation via FGF signaling and epigenetic mechanismsiScienceOctober 21, 2022Ideno H, Imaizumi K, Shimada H, Sanosaka T, Nemoto A, Kohyama J, Okano H.

(19) Hormone regulation of thrombospondin-1 mRNA in porcine granulosa cells in vitroAnimal reproduction scienceSeptember 1, 2022Spicer LJ, Evans JR, Schreiber NB.

(20) Transient and Prolonged Activation of Wnt Signaling Contribute Oppositely to the Pathogenesis of Asherman's SyndromeInternational journal of molecular sciencesAugust 8, 2022Xue X, Li X, Yao J, Zhang X, Ren X, Xu S.

Be the First to Write a Review!

Wnt-5a Protein

Wnt-5a Protein

Wnt-5a Protein

Recombinant Human/Mouse Wnt-5a Protein, CF from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

Citations (183)

Reviews

Recombinant Human/Mouse Wnt-5a Protein, CF from R&D Systems, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map