Fig 1: Loss of Hbα impairs T-lymphocyte intercellular communication.A-H: Splenic T-lymphocytes were isolated and activated with a 1:1 ratio of Dynabeads for 72 hours, replated into transwell inserts over control, RANTES, or CXCL12 media for 4 hours, then total cell counts (A-D) and ratio of cells migrated over control well migration (E-H) were assessed by flow cytometry. I-J: Relative IgM and lambda immunoglobulin concentrations in plasma from 14-day EAE animals (AU: arbitrary units). K-L: Splenic T-lymphocytes were isolated and assessed at 4-hour activated (L) and 24-hour activated (K) for receptor expression by flow cytometry. Statistics measured using Student’s t-test. Statistics (only significant shown) were measured by two-way ANOVA with Šídák’s multiple comparisons test or student’s t-test where appropriate.