Fig 1: Deacetylation of NQO1. The deacetylation of NQO1 was monitored by immunoblot analysis using anti-acetylated lysine antibodies. Ac-NQO1 was generated by incubation of NQO1 with Ac-GSH as described in the Materials and methods section (A) Ac-NQO1 was incubated with purified SIRT1 (470 ng), SIRT2 (100 ng) or SIRT3 (830 ng) in the absence and presence of 1 mM NAD+ for 15 min (B) Ac-NQO1 was incubated with SIRT2 (100 ng) in the absence and presence of 1 mM NAD+ and the sirtuin inhibitor AGK2 (100 µM) for 15min (C) Ac-NQO1 was incubated with HDAC6 (1 µg) or SIRT2 (100 ng) and NAD+ (1 mM) for the indicated times. Reaction conditions are described in the Materials and methods section.