Fig 1: Lateral flow device assembly kit for urinary biomarkers Uromodulin (UMOD) and Osteopontin (OPN). (Left to Right) Biotin/Polystreptavidin (PSA) based LFD for UMOD, Standard LFD for OPN, Diluent tube and Disposable pipette.
Fig 2: Standard curve developed for (a) UMOD using Biotin/Polystreptavidin based Lateral Flow Device and (b) OPN using standard Lateral Flow Device. Three drops of UMOD standard (0–2,000,000 pg/mL) and OPN (0–1,000,000 pg/mL) were run on biotin/polystreptavidin (PSA)/standard lateral flow device respectively for 20 min and the intensity of control line and test line was read using RDS- 2500 reader (DETEKT, USA). The mean ratio of the Test line/ Control line from the LFD was computed, and a semi-log plot was generated to obtain a standard curve. Data is represented as Mean ratio of Test line/Control line intensity (SD).
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Recombinant Human Uromodulin GST (N-Term) Protein