Fig 1: Biochemical characters of KS-58. (A) Binding activity of biotinylated peptides to recombinant K-Ras(G12D) protein in ELISA (n = 4, ± SEM). (B) Binding activity of different concentrations (from the highest 3 µM and subsequent threefold dilution) of Biotin-KS-58 to recombinant Ras proteins as measured by ELISA (n = 4, ± SEM). (C) Inhibition activity of peptides to intracellular Ras–effector proteins interactions. Intracellular PPI in the presence or absence of peptides are detected as luminescence, and the PPI is showed as % value (30 µM, n = 4, ± SEM). (D) Proposed K-Ras(G12D)-inhibition mechanism of KS-58.