Fig 1: Enhanced phosphorylation of p38 and MK2 in Pml -/- cells A, BWT and Pml -/- BMDMs were treated with TNF (100 ng/ml) + zVAD (20 µM) for 15 min on coverslips and stained with anti-phospho-p38 and DAPI before imaging. Images in (A) are representative of two independent experiments (biological replicates). Scale bar, 10 µm. (B) p-p38 total intensity per view was calculated in ImageJ and normalized by total DAPI intensity per view. Nine views were calculated per treatment group. Data show mean ± SD, n = 9. P-values from unpaired t-tests are indicated.C, DWT and Pml -/- BMDMs were stained for p-MK2 and DAPI before and after 15 min of TNF (100 ng/ml) + zVAD (20 µM) treatment. Scale bars indicate 10 µm. Images in (C) are representative of two independent experiments (biological replicates). Quantitation of p-MK2 signal intensity for each group in (D) was performed as in (B). Mean ± SD is shown, n = 9. P-values from unpaired t-tests are indicated.
Supplier Page from Abcam for Recombinant Human MK2 protein