Fig 1: Gαs and Gαi are direct substrates of DHHC5. Palmitoylation of recombinant Gαs (A), Gαi (B), and GAPDH (C) in vitro with mock immunoprecipitation (−), catalytically inactive form of DHHC5 (DHHS5), and DHHC5 are shown. As a negative control, HA was added to cleave all thioester bonds. DHHC total input as determined by Western blotting is shown below the palmitoylation panels.