Fig 1: Survival of NGB-pretreated cells upon LPS stimulation. CATH. a cells were directly incubated with NGB recombinant protein, which were then subjected to LPS stimulation. The survival rate was then evaluated. **P<0.01 vs. control group; #P<0.05 vs. saline +LPS group. NGB, neuroglobin; DEX, dexmedetomidine; LPS, lipopolysaccharide.
Fig 2: miR-320a regulates NGB expression and affects neuronal cell proliferation. (A) Expression of agomiR-320a in CATH.a cells transfected with agomiR-320a. mRNA (B) and protein (C) expression levels of NGB in cells transfected with agomiR-320a. (D) Cell proliferation activity after agomiR-320a transfection. *P<0.05; **P<0.01 vs. agomir-NC group. miR, microRNA; NC, negative control; NGB, neuroglobin.
Fig 3: Bioinformatics prediction analysis. miR, microRNA; NGB, neuroglobin.
Fig 4: NGB expression levels after dexmedetomidine induction. Human neuronal CATH.a cells were directly induced with dexmedetomidine, and the mRNA (A) and protein (B) expression levels of NGB were then detected. *P<0.05, **P<0.01 vs. control (normal saline) group. NGB, neuroglobin; DEX, dexmedetomidine.
Fig 5: Expression of NGB in serum of patients undergoing OPCABG. (A) The mRNA expression levels of NGB in the serum from patients undergoing OPCABG were detected and compared before and after surgery. (B) Protein expression levels of NGB in the serum of patients undergoing OPCABG were detected. The ratio referred to the amount of NGB in the blood of the patient after the operation/the amount before the operation. *P<0.05, **P<0.01 vs. control group; ##P<0.01 vs. before surgery. DEX, dexmedetomidine; NGB, neuroglobin; OPCABG, off-pump coronary artery bypass grafting.
Supplier Page from Abcam for Recombinant Human Neuroglobin protein