Fig 1: Expressionpatternof intracellular CA2 is opposite to its extracellular formA. Western blot showing the intracellular CA2 expression in C tissues and their paired SN tissues in HCC patients. B. Quantification of the intracellular CA2 expression shown in (A). C. Statistical analysis of the intracellular CA2 expression in C tissues and their paired SN tissues on in-house TMAs determined by IHC. D. Representative images of CA2 IHC in a C tissue (score 1) and its paired SN tissue (score 3). Two-tailed paired Student’s t-test (****, P < 0.0001). TMA, tissue microarray; IHC, immunohistochemistry.
Fig 2: Clinical values of serum CA2 in HCC diagnosis and prognosisA. Distribution (upper panel) and ROC curve (lower panel) of serum CA2 levels in HCC patients and healthy volunteers from the training cohort through PRM targeted proteomics. B. Distribution (upper panel) and ROC curve (lower panel) of serum CA2 levels in HCC patients, with and without recurrence, from the validation cohort assessed by ELISA. C. Comparison of cumulative recurrence rate and overall survival rate between the high- and low-CA2 groups in the validation cohort by Kaplan-Meier analysis. D. Distribution of serum CA2 levels in AFP-negative HCC patients. E. Comparison of cumulative recurrence rate between the high- and low-CA2 groups in AFP-negative HCC patients by Kaplan-Meier analysis. F. AUC of the CA2/AFP combination in HCC patients. G. Prognostic value of the CA2/AFP combination in HCC patients. Two-tailed unpaired Student’s t-test (**, P < 0.01; ***, P < 0.001; ****, P < 0.0001). CA2, carbonic anhydrase II; ROC, receiver operating characteristic; AUC, area under the curve; CI, confidence interval; AFP, alpha fetoprotein.
Fig 3: Extracellular CA2 promotes cell migration and invasion through activating EMTA. Representative images and quantification results of migration and invasion of MHCC97L cells treated with exogenous recombinant CA2. B. Cellular morphology of MHCC97L cells treated with exogenous recombinant CA2. Scale bar, 50 μm. C. Western blot showing the expression levels of E-cadherin, N-cadherin, and Zeb1 in MHCC97L cells treated with exogenous recombinant CA2. Two-tailed unpaired Student’s t-test (*, P < 0.05; **, P < 0.01).
Supplier Page from Abcam for Recombinant Human Carbonic anhydrase 2/CA2 protein (Tag Free)