BCIP/NBT Color Development Substrate from Promega

Product Specs
ItemBCIP/NBT Color Development Substrate
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberS3771
Quantity1.25/2.5ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) HIF2α-induced lysyl oxidase safeguards successful pregnancy by remodelling collagens at the feto-maternal interfaceCell Death DisMarch 9, 2026Shizu Aikawa, Ryoko Shimizu-Hirota, Akihiko Sakashita, Xueting He, Daiki Hiratsuka, Chihiro Ishizawa, Rei Iida, Yamato Fukui, Takehiro Hiraoka, Mitsunori Matsuo, et al.fluorescence signals. For colorimetric ISH, anti-DIG-AP (11093274910, Roche, 1:2000) and NBT/BCIP (S3771, Promega) were used. Nuclei were counterstained with 4ʹ,6-diamidino-2-phenylindole (DAPI; Dojindo, 1:500)

(2) Pre-meiotic H1.1 degradation is essential for Arabidopsis gametogenesisThe EMBO JournalJanuary 3, 2026Yanru Li, Danli Fei, Jasmin Schubert, Kinga Rutowicz, Zuzanna Kaczmarska, Alberto Linares, Alejandro Giraldo Fonseca, Sylvain Bischof, Ueli Grossniklaus, Célia Baroux"BCIP/NBT Color Development Substrate,","5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium, Promega, Switzerland",Catalog Number: S3771 Software,Software,Software Fiji,https://fiji.sc/, "Imaris software (Bitplane, Switzerland",https://imaris.oxinst.com/, PyMOL,"Chaudhari

(3) Glis3 as a critical regulator of Pit1-lineages and renal functionsJ Mol Med (Berl)December 29, 2025Giuditta Rurale, Ilaria Gentile, Luca Persani, Federica Marelli[18], and primers are listed in Tab. S2. Staining used 0.35 mg/ml NBT and 0.27 mg/ml BCIP (Promega, S3771) in SB9.5 buffer (0.1 M Tris–HCl, pH 9.2, 50 mM MgCl2, 100 mM NaCl, 0.1% Tween-20). Embryos were post-fixed

(4) SPOROCYTELESS/NOZZLE cooperates with MADS-domain transcription factors to regulate an auxin-dependent network controlling Megaspore-Mother-Cell differentiationNature CommunicationsDecember 14, 2025Alex Cavalleri, Chiara Astori, Silvia Manrique, Greta Bruzzaniti, Cezary Smaczniak, Chiara Mizzotti, Alessandro Ruiu, Mattia Spanò, Andrea Movilli, Veronica Gregis, et al.number: 11093274910) and colour detection (BCIP/NBT Colour Development Substrate, Promega, cat. number: S3771) were performed according to the manufacturer’s instructions. ANT, PIN1 and ARF9 anti-sense probes were

(5) Shared regulatory networks link vein positioning and eyespot ring formation in butterfliesCommunications BiologyDecember 13, 2025Tirtha Das Banerjee, Antónia Monteiroalkaline-phosphatase buffer (see Supplementary Table S4 for composition) supplemented with NBT-BCIP (Promega, Cat No. S3771) and incubated in the dark till the development of color. The wings were imaged under a Leica DMS1000

(6) Dax1 in AgRP neurons regulates thermogenesis via GR-HDAC3-mediated CRFR1 suppressionMolecular MetabolismSeptember 27, 2025Zhuodu Wei, Jooseon Cha, Seunghee Leecontaining 2% BSA. On the next day, signal was developed using NBP/BCIP substrate solution (Promega, Cat# S3771), and sections were mounted with Permount. For fluorescence in situ hybridization (FISH), digoxigenin-labeled

(7) Tat-fimbriae (“tafi”): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathwayiScienceFebruary 21, 2025Anna V. Galeva, Dahe Zhao, Alexey S. Syutkin, Marina Yu Topilina, Sergei Yu Shchyogolev, Elena Yu Pavlova, Olga M. Selivanova, Igor I. Kireev, Alexey K. Surin, Gennady L. Burygin, et al.Q5® High-Fidelity DNA Polymerase,NEB,M0491S SphI,NEB,R0182S DNase I,Thermo scientific,EN0521 BCIP/NBT Color Development Substrate,Promega,S3771 IPTG,Thermo scientific,R0392 L-Tryptophan,Merck,T0254 Bacto™ Casamino Acids,Thermo scientific,223050 Brilliant

(8) Endometrial zinc transporter Slc39a10/Zip10 is indispensable for progesterone responsiveness and successful pregnancy in micePNAS NexusFebruary 10, 2025Yui Kawata, Jumpei Terakawa, Ayuu Takeshita, Takafumi Namiki, Atsuko Kageyama, Michiko Noguchi, Hironobu Murakami, Toshiyuki Fukada, Junya Ito, Naomi Kashiwazaki2% CS-FBS, 1 nM E2, and 1 μM P4. ALP staining with BCIP/NBT Color Development Substrate solution (#S3771, Promega, Madison, WI, USA) was performed to detect the successful decidual reaction as described (72,

(9) Single-cell transcriptomics of vomeronasal neuroepithelium reveals a differential endoplasmic reticulum environment amongst neuronal subtypeseLifeDecember 13, 2024G V S Devakinandan, Mark Terasaki, Adish Danicompound, drug",TSA Plus Fluorescein,Akoya,NEL741001KT, Commercial assay or kit,BCIP/NBT kit,Promega,S3771, Other,Bovine Serum Albumin,Jackson ImmunoResearch Inc,Cat#001-000-173RRID:AB_2336947,Blocking reagent

(10) Co-option of the trichome-forming network initiated the evolution of a morphological novelty in Drosophila eugracilisCurrent BiologyNovember 18, 2024Gavin Rice, Tatiana Gaitán-Escudero, Kenechukwu Charles-Obi, Julia Zeitlinger, Mark RebeizLife Technologies,Cat# P2075 Anti-digoxigenin AP-conjugate,Roche,Cat# 11093274910 BCIP/NBT,Promega,S3771 EnGen® sgRNA Synthesis Kit,NEB,Cat # NC1194374 EnGen Spy Cas9 NLS,NEB,Catalog # M0646M MEGACLEAR Transcription Clean-Up KIT

(11) Conserved regulatory switches for the transition from natal down to juvenile feather in birdsNature communicationsMay 16, 2024Chen CK, Chang YM, Jiang TX, Yue Z, Liu TY, Lu J, Yu Z, Lin JJ, Vu TD, Huang TY, et al.NaCl, 0.1% Tween 20) buffer (2 times, 15 min each). Color development was achieved using NBT/BCIP (S3771, Promega) chromogenic substrates. Construction and misexpression of dominant negative form of the candidate

(12) Development of monoclonal antibodies against oropouche virus and its applicability to immunohistochemical diagnosisVirology JournalApril 8, 2024Ana Paula Andreolla, Alessandra Abel Borges, Seigo Nagashima, Caroline Busatta Vaz de Paula, Lucia de Noronha, Nilson I.T. Zanchin, Juliano Bordignon, Claudia Nunes Duarte dos Santosdiluted in 5% non-fat milk in TBS-T. The reaction was developed using the BCIP/NBT reagent (cat. number S3771, Promega, USA) according to the manufacturer’s recommendations. Identification of the OROV protein

(13) Calcium-binding protein 7 expressed in muscle negatively regulates age-related degeneration of neuromuscular junctions in miceiScienceFebruary 16, 2024Takahiro Eguchi, Tohru Tezuka, Yuji Watanabe, Akane Inoue-Yamauchi, Hiroshi Sagara, Manabu Ozawa, Yuji Yamanashi342 Anti-Digoxigenin-AP Fab fragments,Merck,Cat# 11093274910 BCIP/NBT Color Development Substrate,Promega,Cat# S3771 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, RNeasy Mini

(14) Performance of plant-produced RBDs as SARS-CoV-2 diagnostic reagents: a tale of two plant platformsFrontiers in Plant ScienceJanuary 4, 2024Mattia Santoni, Noemi Gutierrez-Valdes, Denise Pivotto, Elena Zanichelli, Anthony Rosa, Guillermo Sobrino-Mengual, Juliette Balieu, Patrice Lerouge, Muriel Bardor, Riccardo Cecchetto, et al.secondary antibody (2-1503-001, IBA Lifesciences) diluted 1:2,000, followed by BCIP/NBT substrate (S3771, Promega), and Odyssey DLs Imaging System (Li-cor Biosciences, USA) was used for detection. RBD purification

(15) The mitochondrial DNA common deletion as a potential biomarker of cancer-associated fibroblasts from skin basal and squamous cell carcinomasScientific ReportsJanuary 4, 2024Gabriele A. Fontana, Michael R. MacArthur, Nadezhda Rotankova, Michela Di Filippo, Hans-Dietmar Beer, Hailey L. Gahlon,Pierce BCA protein assay kit,ThermoFisher Scientific,23225, Commercial assay/kit,BCIP/NBT Color Development Substrate,Promega,S3771, Commercial assay/kit,ECL Western Blotting Substrate,Promega,W1001, Commercial assay/kit,Citrate Synthase Activity Assay Kit

(16) Multiple intra-articular injections with adipose-derived stem cells for knee osteoarthritis cause severe arthritis with anti-histone H2B antibody productionRegenerative therapyDecember 1, 2023Hosono Y, Kuwasawa A, Toyoda E, Nihei K, Sato S, Watanabe M, Sato M.developed with BCIP/NBT Color Development Substrate following the manufacturer's instructions (Promega S3771). 2.6 Enzyme-linked immunosorbent assay (ELISA) to measure anti-histone H2B Ab titer Synovial fluid

(17) Spontaneous p53 activation in middle-aged C57BL/6 mice mitigates the lifespan-extending adaptive response induced by low-dose ionizing radiationnpj AgingNovember 7, 2023Masaoki Kohzaki, Keiji Suzuki, Akira Ootsuyama, Ryuji Okazakigoat-anti-rabbit IgG-AP (1:2000 dilution; A3812, A3688, Sigma-Aldrich) secondary antibodies. BCIP/NBT (S3771, Promega) was used to develop the blots. The band intensities were analyzed with ImageJ-3 software.

(18) Antiviral Evaluation of Dispirotripiperazines against Hepatitis B VirusJournal of Medicinal ChemistrySeptember 14, 2023Thane Jones, John E. Tavis, Qilan Li, Olga Riabova, Natalia Monakhova, Daniel P. Bradley, Thomas R. Lane, Vadim Makarov, Sean Ekinsantibody (Promega, S3721) and detected by using BCIP and NBT Color Development Substrate (Promega, S3771). Bands were quantified by densitometry. Data curation Datasets for HBV inhibition (EC50/IC50 for

(19) The Trophoblast Compartment Helps Maintain Embryonic Pluripotency and Delays Differentiation towards CardiomyocytesInt J Mol SciAugust 4, 2023Xiang Zhao, Bethany N. Radford, Mark Ungrin, Wendy Dean, Myriam Hembergerphosphatase staining was performed using 5-bromo-4-chloro-3-indolyl phosphate-nitro blue tetrazolium kit (S3771, Promega, Madison, WI, USA) according to the manufacturers protocol. Colonies were imaged and scored

(20) Spatial and temporal regulation of Wnt signaling pathway members in the development of butterfly wing patternsScience AdvancesJuly 28, 2023Tirtha Das Banerjee, Suriya Narayanan Murugesan, Heidi Connahs, Antόnia Monteirobuffer supplemented with bromochloroindolyl phosphate–nitro blue tetrazolium (Promega, catalog no. S3771) and left for 4 to 6 hours in the dark for color to develop. Once the color developed, wings were washed

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