Cultrex Basement Membrane Extract, Pathclear from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCultrex Basement Membrane Extract, Pathclear
CompanyR&D Systems, a Bio-Techne Brand
Price $231.00Supplier Page View Company Product Page
Catalog Number3432-005-01
Quantity5 mL
ApplicationsBioactivity
Format12 - 18 mg/mL
TypeBasement Membrane Extract
StorageProduct is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 ug/ml gentamicin sulfate. Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Mebendazole Attenuates Cellular Invasion in a 3D Culture Model of Meningioma by Disrupting Rho-GTPase-Mediated Microtubule FunctionOncology ResearchApril 22, 2026Munro Matthew James, López Vásquez Clara Clara Elena, Wickremesekera Agadha, Chan Alex Ho Chuen, Gray Clint LeeCultrex Basement Membrane Extracts (BME) with a protein concentration of 8–12 mg/mL (R&D Systems, cat# 3432-005-01, Minneapolis, MN, USA) into each well using frozen P200 pipette tips and working on ice. The plate was

(2) Protocol for generating a 3D hydrogel-based tumor resection model in vitro using pancreatic cancer cellsSTAR ProtocMarch 20, 2026Lea Miebach, Marten Hagge, Linus Hübner, Sander BekeschusBiotec,Cat# P04-80100 Phosphate buffered saline,Pan Biotec,Cat# P04-36500 Hydrogel,R&D Systems,Cat#3432-005-01 DAPI,Carl Roth,Cat#6335.1 Vybrant DiD Cell Labeling Solution,Thermo Fisher,Cat#V22887 SYTOX Green,Thermo Fisher

(3) Vessels encapsulating tumor clusters promote non-invasive metastasis of hepatocellular carcinoma by shaping an immunosuppressive microenvironmentJournal of Clinical InvestigationFebruary 16, 2026Bi-Yu Huang, Zheng-Qi Mi, Xiao-Yu Zhang, Yu-Chen Ji, Meng-Zhi Wu, Zi-Feng Cheng, Chen Xie, Shuai He, Jing Zhu, Jian-Hong Fang, et al.of 5 weeks. For Treg depletion assay, Hepa1-6 cells (6 × 105) were resuspended in 25 μL of Matrigel (3432-005-01, R&D Systems, MN, USA) /DMEM mixture (1:1 volume) and then inoculated into the liver of a C57BL/6J mouse.

(4) TGFβ1 Activates Lnc‐APUE to Promote Tumor Metastasis via the Alu Element‐Driven STAU1‐Mediated Decay of CDH1 mRNAAdvanced ScienceFebruary 3, 2026Song‐Yang Li, Jia‐Hui Huang, Jin‐E Yang, Yi‐Hang Li, Juan‐Zhen Hong, Ting‐Ting Wang, Ying‐Lei Chi, Meng‐Zhi Wu, Wei Wang, Ying Zhu, et al.polycarbonate membrane (Corning). For invasion assays, the upper chamber was pre‐coated with Matrigel (3432‐005‐01, R&D Systems) to establish matrix barriers on the membrane. Tumor cells were resuspended in 100 µL of

(5) Granuloma dual RNA-seq reveals composite transcriptional programs driven by neutrophils and necrosis within tuberculous granulomasScience AdvancesJanuary 23, 2026Gopinath Viswanathan, Erika J. Hughes, Mingyu Gan, Ana María Xet-Mull, Jacob P. Lowy, Charlie J. Pyle, Graham Alexander, Devjanee Swain-Lenz, Qingyun Liu, David M. Tobinplate (GBO, #55090) covered with 40 μl of Cultrex Basement Membrane Extract (5 μg/ml; R&D Systems, #3432-005-01) in L-15. The granulomas were then embedded in a top medium containing 5% fetal bovine serum (Sigma-Aldrich,

(6) Interaction of lncRNA LENT with DHX36 regulates translation and suppresses autophagy in melanomaCell Death & DiseaseDecember 19, 2025Alexandre Haller, Giovanni Gambi, Mattia D’Agostino, Guillaume Davidson, Antonin Lallement, Gabrielle Mengus, Chadia Nahy, Nadia Messaddeq, Guillaume Bec, Angelita Simonetti, et al.with 3 × 106 IGR37 cells resuspended in 100 µL of 1x PBS + Cultrex Basement Membrane Extract (ref. 3432–005–01; R&D Systems) with a 1:1 ratio. Tumor growth was monitored by caliper measurement every 2 days, and

(7) TRPM7 Deficiency Accelerates Vascular Senescence by Inhibiting H3K18 LactylationAging CellNovember 1, 2025Yue Wang, Jing Chen, Xuan Wang, Jin Li, Shujun Yang, Lingping Zhu, Zhenyu Li, Chuanchang Li, Wanzhou Wu, Yongping Bai(Sigma–Aldrich, 42364) was used for labeling the cell membrane. We precoated the μ‐slide with 10 μL of Matrigel (3432‐005‐01, R&D systems) for 30 min at 37°C. Next, a total of 5 × 103 HUVECs resuspended in 50 μL of the corresponding

(8) Genome-wide mapping of arsenic-activated Nrf2 reveals metabolic and epigenetic reprogramming in induced pluripotent stem cellsRedox BiologyOctober 1, 2025Akimasa Seno, Zhuoyue Bi, Lisa Polin, Ziqi Liu, Yiran Qiu, Wenxuan Zhang, Aashna Pawar, Chitra Thakur, Masaharu Seno, Ziwei Wang, et al.phosphate-buffered saline (PBS) mixed with phenol red-free Cultrex basement membrane extract (R&D Systems, cat#3432-005-01) at a final BME concentration of 10 mg/mL. A total of 1 × 106 cells in 200 μL of the cell/BME mixture

(9) WNT5a export onto extracellular vesicles studied at single‐molecule and single‐vesicle resolutionThe FEBS JournalSeptember 1, 2025Antonia Schubert, Ajaree Mongkolsittisilp, Andrei Kobitski, Matthias Schulz, Oksana Voloshanenko, Meike Schaffrinski, Nadine Winkler, Michelle Neßling, Karsten Richter, Dominique Kranz, et al.previously reported [48]. 105 MCF7 cells were seeded in triplicates in EV‐free medium onto an ECM (3432‐005‐01; Cultrex PathClear Basement Membrane Extract, Bio‐Techne, Minneapolis, MN, USA) coated polycarbonate

(10) The ALDH2/PolG2 axis enhances mitochondrial biogenesis via transcriptional regulation of Nrf2 and promotes chemotherapy resistance in acute myeloid leukaemiaCell death & diseaseAugust 13, 2025Hu X, Hu T, Cao S, Jiang L, Zhou Y, Fang Q, Wang J.established. In brief, KG1avector/Luc and KG1aALDH2/Luc cells (5 × 106/mouse) were first mixed with Matrigel (3432-005-01, R&D Systems Bio-Techne) at a 1:1 ratio. The mixture was subsequently injected subcutaneously into the

(11) Antitumor CD4+ T Helper 1 Cells Target and Control the Outgrowth of Disseminated Cancer CellsCancer Immunology ResearchMay 2, 2025Ganesan Ramamoorthi, Marie Catherine Lee, Carly M. Farrell, Colin Snyder, Saurabh K. Garg, Amy L. Aldrich, Vincent Lok, William Dominguez-Viqueira, Sy K. Olson-Mcpeek, Marilin Rosa, et al.mice, or TUBO cells (2.5 × 105) were resuspended in PBS, plus an equal volume of Cultrex BME (Cat. No. 3432-005-01, R&D Systems), and mixed with EGF (5 μg; Cat. No. 315-09, PeproTech) and FGF-basic (5 μg; Cat. No. 450-33,

(12) Investigating synthetic lethality and PARP inhibitor resistance in pancreatic cancer through enantiomer differential activityCell Death DiscovMarch 16, 2025Mirco Masi, Laura Poppi, Viola Previtali, Shannon R. Nelson, Kieran Wynne, Giulia Varignani, Federico Falchi, Marina Veronesi, Ennio Albanesi, Daniele Tedesco, et al.Organoids were established by resuspending cells in Cultrex Basement Membrane (BME) (R&D System Biotechne, 3432-005-01), then 20 μL of organoid/BME solution was pipetted into a 24-multiwell polyHEMA coated plate. The plate

(13) Are 3D Tumor Cell Spheroids a Utile System for the In Vitro Evaluation of Diagnostic Radiotracers?ACS OmegaDecember 31, 2024Benedikt Judmann, Florian Keller, Björn Wängler, Ralf Schirrmacher, Rüdiger Rudolf, Carmen Wängler(Coll1; Roche, 11179179001) or 2.5% basal membrane extract (BME; Biotechne, Cultrex PathClear BME, 3432-005-01) before seeding 900 000 cells per well in 600 μL volume. After 30 min at 37 °C, supernatants were removed,

(14) The role of mesenchymal cells in cholangiocarcinomaDisease Models & MechanismsDecember 1, 2024Mireia Sueca-Comes, Elena Cristina Rusu, Jennifer C. Ashworth, Pamela Collier, Catherine Probert, Alison Ritchie, Marian Meakin, Nigel P. Mongan, Isioma U. Egbuniwe, Jesper Bøje Andersen, et al.(cancer cells:MSCs). Mouse-derived basement membrane extract (BME) from Cultrex PathClear (Bio-techne, 3432-005-01) was thawed overnight on ice at 4°C and added at a concentration of 100 µg/ml. A volume of 200 µl (equivalent

(15) Targeting CD93 on monocytes revitalizes antitumor immunity by enhancing the function and infiltration of CD8(+) T cellsJournal for immunotherapy of cancerOctober 23, 2024Jiang D, Huang A, Zhu BX, Gong J, Ruan YH, Liu XC, Zheng L, Wu Y.model: 1×105 Luminescence-Hepa1-6 cells were suspended in 25 µL of 66.7% basement membrane extract (3432-005-01, R&D Systems, USA), and intrahepatically injected into the left lobe of livers of anesthetized 6-week-old

(16) Human neural stem cells derived from fetal human brain communicate with each other and rescue ischemic neuronal cells through tunneling nanotubesCell death & diseaseSeptember 1, 2024Capobianco DL, De Zio R, Profico DC, Gelati M, Simone L, D'Erchia AM, Di Palma F, Mormone E, Bernardi P, Sbarbati A, et al.

(17) Synergistic induction of mitotic pyroptosis and tumor remission by inhibiting proteasome and WEE family kinasesSignal Transduction and Targeted TherapyJuly 12, 2024Zhan-Li Chen, Chen Xie, Wei Zeng, Rui-Qi Huang, Jin-E Yang, Jin-Yu Liu, Ya-Jing Chen, Shi-Mei Zhuangat early stage of tumor development, HeLa cells (5 × 106) were resuspended in 100 μL DMEM/Matrigel (3432-005-01; R&D Systems, Minneapolis, MN, USA) and then subcutaneously injected into both side of the posterior

(18) A feedback loop driven by H3K9 lactylation and HDAC2 in endothelial cells regulates VEGF-induced angiogenesisGenome BiologyJune 25, 2024Wei Fan, Shuhao Zeng, Xiaotang Wang, Guoqing Wang, Dan Liao, Ruonan Li, Siyuan He, Wanqian Li, Jiaxing Huang, Xingran Li, et al.pretreated under different conditions for 18 h. We precoated 96-well plates with 50 µl of Matrigel (3432–005-01, R&D systems) for 40 min at 37 ℃. Next, a total of 1 × 104 HRMECs resuspended in 100 µL of the corresponding

(19) Branched-chain amino acids and L-alanine supplementation ameliorate calcium dyshomeostasis in sarcopenia: New insights for nutritional interventionsFrontiers in PharmacologyJune 19, 2024Elena Conte, Paola Mantuano, Brigida Boccanegra, Paola Imbrici, Giorgia Dinoi, Roberta Lenti, Ornella Cappellari, Donato Cappetta, Antonella De Angelis, Liberato Berrino, et al.extracellular matrix basement membranes (Cultrex, BioTechne, Minneapolis, MN, United States, cod. #3432–005-01). The coverslips were kept in an incubator with 5% CO2 at 30 °C, and the adhesion of the fibers to the

(20) Protocol for high throughput 3D drug screening of patient derived melanoma and renal cell carcinomaSLAS DiscoveryApril 1, 2024Luis M. Ortiz Ortiz Jordan, Virneliz Fernández Vega, Justin Shumate, Adam Peles, Jordan Zeiger, Louis Scampavia, Timothy P. Spicer(Avantor cat. no. 970868–085) Cultrex Basement Membrane Extract, PathClear (Bio-techne, cat. no. 3432-005-01) Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, PathClear (Bio-techne, cat. no.

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Cultrex Basement Membrane Extract, Pathclear from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Cultrex Basement Membrane Extract, Pathclear from R&D Systems, a Bio-Techne Brand

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