Cultrex Stem Cell Qualified RGF Basement Membrane Extract from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCultrex Stem Cell Qualified RGF Basement Membrane Extract
CompanyR&D Systems, a Bio-Techne Brand
Price $285.00Supplier Page View Company Product Page
Catalog Number3434-005-02
Quantity5 mL
Format12 - 18 mg/ml
TypeBasement Membrane Extract
StorageProduct is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 ug/ml gentamicin sulfate. Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines
ApplicationsBioactivity

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) VMAT2 dysfunction impairs vesicular dopamine uptake, driving its oxidation and α-synuclein pathology in DJ-1-linked Parkinson's neuronsSci AdvFebruary 13, 2026Leonie M. Heger, Francesco Gubinelli, Andreas J. Huber, Aida Cardona-Alberich, Matteo Rovere, Ulf Matti, Stephan A. Müller, Sankarshana R. Nagaraja, Lena Jaschkowitz, Martina Schifferer, et al.written and informed consent. iPSC cultures were kept on plates coated with Cultrex (R&D Systems, no. 3434-005-02), and the antibiotic-free mTESR Plus medium (Stemcell Technologies, no. 100-0276) was replenished every

(2) Protocol for culturing olfactory epithelium organoids supporting neuronal differentiationSTAR ProtocolsDecember 1, 2025Juliana Gutschow Gameiro, Jussi Virtanen, Liam Lee, James E. Schwob, Marco Aurélio Fornazieri, Brian Lin15630080 Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract,Bio-techne,Cat# 3434-005-02 Trypan blue,Thermo Fisher Scientific,Cat# 15250061 PBS pH 7.4 1x,Thermo Fisher Scientific,Cat# 10010023 Bovine serum albumin (BSA

(3) Strain-specific tropism and transcriptional responses of enterovirus D68 infection in human spinal cord organoidsFrontiers in MicrobiologyNovember 19, 2025Nathânia Dábilla, Sarah Maya, Colton McNinch, Taylor Eddens, Patrick T. Dolan, Megan Culler Freeman(Tocris, 1254). They were seeded and passaged in flasks coated with 150 μg/mL Cultrex (R&D Systems, 3434-005-02). The 3-DiSC hSCO were propagated and differentiated as described in Aguglia et al. (2023) for up to

(4) HDAC inhibitors engage MITF and the disease-associated microglia signature to enhance amyloid β uptakeBrain, Behavior, and ImmunityOctober 1, 2025Verena Haage, John F. Tuddenham, Alex Bautista, Frankie Garcia G., Charles C. White, Ronak Patel, Natacha Comandante-Lou, Victoria Marshe, Jennifer Griffin, Ye Zhou, et al.for 24hrs. The same day, wells of 96-well plates were coated with 1:100 Cultrex (R&D Systems, Cat #: 3434-005-02)(in ddH2O) overnight. The next day, iMGs were lifted of the plate using resuspension, cell viability

(5) Human Stem Cell-Derived β-cells Expressing An Optimized CD155 Reduce Cytotoxic Immune Cell Function for Application in Type 1 DiabetesbioRxiv : the preprint server for biologyAugust 15, 2025Brown ME, Barra JM, Pina MR, Proia J, Brusko TM, Russ HA.

(6) Immune cells promote paralytic disease in mice infected with enterovirus D68Journal of Clinical InvestigationAugust 1, 2025Mikal A. Woods Acevedo, Jie Lan, Sarah Maya, Jennifer E. Jones, Isabella E. Bosco, John V. Williams, Megan Culler Freeman, Terence S. Dermodysupplemented to contain 10 μM Y-27632 (Tocris, 1254) in flasks coated with 150 μg/mL Cultrex (R&D Systems, 3434-005-02). To prepare 3-DiSC hSCOs, a single-cell suspension of iPSCs was prepared using ACCUMAX (STEMCELL Technologies),

(7) Strain-Specific Tropism and Transcriptional Responses of Enterovirus D68 Infection in Human Spinal Cord OrganoidsbioRxivJune 28, 2025Nathânia Dábilla, Sarah Maya, Colton McNinch, Taylor Eddens, Patrick T. Dolan, Megan Culler Freeman(Tocris, 1254). They were seeded and passaged in flasks coated with 150 μg/mL Cultrex (R&D Systems, 3434-005-02). The 3-DiSC hSCO were propagated and differentiated as described in Aguglia et al. [21] for up to 24

(8) Quiescent horizontal basal stem cells act as a niche for olfactory neurogenesis in a mouse 3D organoid modelCell Reports MethodsJune 16, 2025Juliana Gutschow Gameiro, Constantin A. Hintschich, Agnès Dekeyser, Valérie Hox, James E. Schwob, Eric H. Holbrook, Marco Aurélio Fornazieri, Brian LinQualified Reduced Growth Factor Basement Membrane Extract (4% vol/vol with 9 mg/mL of protein, Bio-techne #3434-005-02).26 Media was gently changed every other day, with care taken not to aspirate the Cultrex gel that forms

(9) Protocol for establishing inducible CRISPR interference system for multiple-gene silencing in human pluripotent stem cellsSTAR ProtocolsSeptember 20, 2024Satoshi Matsui, Joseph R. Shiley, Marissa Granitto, Katie Ludwig, Morgan Buckley, Sandra Koigi, Gerardo Mirizio, Yueh-Chiang Hu, Christopher N. Mayhew, Makiko IwafuchiAlternatives: Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (Bio-Techne, #3434-005-02) is also a suitable coating for hPSC culture. The final concentration of Cultrex is 40 μg/mL. After

(10) Single-cell RNA sequencing reveals placental response under environmental stressNature communicationsAugust 2, 2024Van Buren E, Azzara D, Rangel-Moreno J, Garcia-Hernandez ML, Murphy SP, Cohen ED, Lewis E, Lin X, Park HR.iPSC Feeder-Free Growth Medium (MD-0019, iXCells Biotechnologies) on plates precoated with Cultrex (3434-005-02, R&D Systems), adhering strictly to the supplier’s guidelines. The iPSCs underwent differentiation into

(11) Osteopontin drives neuroinflammation and cell loss in MAPT-N279K frontotemporal dementia patient neuronsCell Stem CellMay 2, 2024Osama Al-Dalahmah, Matti Lam, Julie J. McInvale, Wenhui Qu, Trang Nguyen, Jeong-Yeon Mun, Sam Kwon, Nkechime Ifediora, Aayushi Mahajan, Nelson Humala, et al.Media,ThermoFisher,Cat#10888–022 Collagenase IV,ThermoFisher,Cat#17104019 Accutase,ThermoFisher,Cat#A11105–01 Cultrex,Biotechne,Cat#3434–005-02 ReLeSR,Stem Cell Technologies,Cat#05872 Knockout DMEM,ThermoFisher,Cat#10829018 Knockout Serum Replacement,ThermoFisher,Cat#10828010 Glutamax,ThermoFisher,Cat#35050061 Penicillin/streptomycin/glutamine,ThermoFisher,Cat#10378016 Normal goat serum

(12) Ex-vivo drug screening of surgically resected glioma stem cells to replace murine avatars and provide personalise cancer therapy for glioblastoma patientsF1000ResearchMarch 11, 2024Hannah Gagg, Sophie T. Williams, Samantha Conroy, Katie N. Myers, Connor McGarrity-Cottrell, Callum Jones, Thomas Helleday, Juha Rantala, Ola Rominiyi, Sarah J. Danson, et al.(A2153-100G) BreatheEasy Sealing Membrane,Sigma (Z380059-1PAK) Cultrex Stem Cell Qualified RGF BME,R&D Systems (3434-005-02) Dimethyl Sulfoxide (DMSO),Fisher Scientific (BP231-100) E1-ClipTip 16 Multichannel pipette,Thermo

(13) Immune evasion of dormant disseminated tumor cells is due to their scarcity and can be overcome by T cell immunotherapiesCancer cellJanuary 8, 2024Goddard ET, Linde MH, Srivastava S, Klug G, Shabaneh TB, Iannone S, Grzelak CA, Marsh S, Riggio AI, Shor RE, et al.Concentrator,Takara,Cat#631456 Puromycin,Millipore Sigma,Cat#P8833-25MG Cultrex Reduced Growth Factor Basement Membrane Extract,R&D Systems,Cat#3434-005-02 recombinant human IL-2,PeproTech,Cat#200-02 recombinant human IFNγ,PeproTech,Cat#300-02 Phorbol 12-myristate 13-acetate

(14) Contemporary enterovirus-D68 isolates infect human spinal cord organoidsmBioAugust 31, 2023Aguglia G, Coyne CB, Dermody TS, Williams JV, Freeman MC.Pluripotent stem cell medium SFM SF/FF (ACS-3002) in flasks coated with 150 µg/mL Cultrex (R&D Systems, 3434-005-02). These cells were obtained commercially through the ATCC. Hela 7b cells were maintained in MEM medium

(15) Ex-vivo drug screening of surgically resected glioma stem cells to replace murine avatars and provide personalise cancer therapy for glioblastoma patientsF1000ResearchJanuary 1, 2023Gagg H, Williams ST, Conroy S, Myers KN, McGarrity-Cottrell C, Jones C, Helleday T, Rantala J, Rominiyi O, Danson SJ, et al.(A2153-100G) BreatheEasy Sealing Membrane,Sigma (Z380059-1PAK) Cultrex Stem Cell Qualified RGF BME,R&D Systems (3434-005-02) Dimethyl Sulfoxide (DMSO),Fisher Scientific (BP231-100) E1-ClipTip 16 Multichannel pipette,Thermo

(16) Efficient Derivation of Excitatory and Inhibitory Neurons from Human Pluripotent Stem Cells Stably Expressing Direct Reprogramming FactorsCurr ProtocJune 1, 2021Song S, Ashok A, Williams D, Kaufman M, Duff K, Sproul A.12320–033) Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (R&D Systems, cat. no. 3434–005-02; see recipe) ReLeSR (STEMCELL Technologies, cat. no. 05872) Accutase Cell Detachment Solution (Innovative

(17) Syrbactin proteasome inhibitor TIR-199 overcomes bortezomib chemoresistance and inhibits multiple myeloma tumor growth in vivoLeukemia ResearchJanuary 1, 2020Marquicia R. Pierce, Reeder M. Robinson, Tannya R. Ibarra-Rivera, Michael C. Pirrung, Nathan G. Dolloff, AndrÃ© S. Bachmannwith 1 × 106 ARD cells suspended in a 1:1 mixture of PBS/matrigel (R&D Systems, RGF BME PathClear®, #3434-005-02, MM, USA) in the right flank. Tumors were allowed to grow until they reached 200 mm3 and the mice were

(18) Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiationStem cells translational medicineJanuary 1, 2013Tucker BA, Anfinson KR, Mullins RF, Stone EM, Young MJ.

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