M13mp18 Single-stranded DNA from New England Biolabs

Product Specs
ItemM13mp18 Single-stranded DNA
CompanyNew England Biolabs
Price $46.00 Inquire
Catalog NumberN4040S
Quantity10 µg
ApplicationsDNA Manipulation
Temperature-20 C
Nucleic Acid TypeCloning Vectors

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Reset-free DNA logic circuits for real-time input processing and memorySci AdvApril 3, 2026Junho Sim, Taehoon Kim, Woojin Kim, Sangeun Jeong, Eunjin Choi, Sion Kim, Hansol Choi, Sung Sun Yim, Yeongjae ChoiMETHODS Materials The M13mp18 single-stranded DNA scaffold was purchased from New England Biolabs (cat. no. N4040S). The circuit probes, inputs, and staple oligonucleotides with extensions were purchased from Macrogen

(2) Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cellsbioRxivMarch 27, 2026Sylvi Stoller, Asmita Jha, Joerg Bewersdorf, Florian Schuederreaction with 50 μL total volume, consisting of 10 nM M13mp18 scaffold strand (New England BioLabs, N4040S), 10 nM biotinylated staples (IDT) (Suppl. Table 4), and 100 nM folding staples (IDT) and 1 μM of docking

(3) Dye-cycling DNA origami rotors for long-term tracking of transcription at base-pair resolutionbioRxivJanuary 28, 2026Amanda Wacker, Ryan J. Fantasia, Brian J. Tenner, Jerry Wu, Nicholas A. Monell, Pallav Kosurioverhangs, and linkers. DNA origami staple strands M13mp18 viral DNA purchased from New England Biolabs (N4040S). DNA origami rotors were folded in a buffer containing 10 mM Tris, 1 mM EDTA, and 18 mM MgCl2. Folding

(4) Mechanisms of HSV-1 helicase-primase inhibition and replication fork complex assemblyCellJanuary 22, 2026Zishuo Yu, Pradeep Sathyanarayana, Cong Liu, Joel M.J. Tan, Pan Yang, Biswajit Das, Side Hu, Xiaoyi Fan, Chenggong Ji, Sandra K. Weller, et al.5′-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGT-3′ primer with 60 nt mismatch poly T, and M13mp18 ssDNA (NEB Cat# N4040S) were annealed to generate the ssDNA vector with a fork (Table S2). Rolling circle assays with 6-FAM-labeled

(5) Light‐Guided Molecular Patterning for High‐Throughput Single‐Molecule Mechanical CharacterizationSmallJanuary 19, 2026Hansol Choi, Andrew Ward, Wesley P. WongS1). The DNA nanoswitch construct for force spectroscopy is synthesized with circular M13mp18 ssDNA (N4040S, New England Biolabs). To linearize the circular DNA, 10 µL of 110 nm M13mp18 ssDNA, 7 µL of 10 µm 20

(6) Investigating Polarity Effects in DNA Base StackingJACS AuDecember 22, 2025Jibin Abraham Punnoose, Chai S. Kam, Tristan Melfi, Sweta Vangaveti, Alan A. Chen, Ken HalvorsenPAGE-purified (www.idtdna.com). M13mp18 circular single-stranded DNA (New England Biolabs, catalog #N4040S) served as the scaffold for construct synthesis. Construct Preparation The circular single-stranded

(7) A denaturation-free protocol for in situ visualization of short nuclear DNA sequences using padlock probes with rolling-circle amplificationPLoS OneOctober 28, 2025Ryoyo Ikebuchi, Lu Xi, Dimitra Bouri, Valeriia Svintytska, Hanna Davies, Pawel Olszewski, Bozena Bruhn-Olszewska, Jan P. Dumanski, Ulf LandegrenNew England Biolabs) according to the manufacturer’s protocol and stored at −20°C until use. One nM ssDNA M13 (N4040S, New England Biolabs), dsDNA supercoiled or nicked M13 DNA, serving as ligation targets, were incubated

(8) A DNA nicking Class 1 OLD family nuclease mediates phage defense in Vibrio cholerae and is countered by a phage-encoded inhibitorNucleic Acids ResAugust 28, 2025Kishen M Patel, Kimberley D Seedmapping.” Similarly, single-stranded DNA (ssDNA) (from M13 phage) was ordered from New England Biolabs (N4040S), extracted, and eluted into water. Total RNA was prepped from V. cholerae E7946 as described previously

(9) Molecular logic gates based on programmable self-assembly of DNA origami triangles for the detection of nucleic acid moleculesSci RepAugust 9, 2025Si Sun, Jin-Da Wen, Qian-Ru Xiao, Xiao-Li Qiang, Xiao-Long Shiin this study is single-stranded M13mp18 DNA which was purchased from New England Biolabs (Catalog # N4040S) at 250 µg/mL in 1× TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). Staple stands were purchased from

(10) Competition for the nascent leading strand shapes the requirements for PCNA loading in the replisomeThe EMBO JournalFebruary 28, 2025Emma E Fletcher, Morgan L Jones, Joseph T P Yeeles/5Phos/GCTATGTGGTAGGAAGTGAGAATTGGAGAGTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGGAAAGAATGTTGGTGAGGGTTGGGAAGTGGAAGGATGGGCTCGAGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT","Baris et al, 2022",N/A M13mp18 ssDNA,New England Biolabs,N4040S ZN3,"Taylor and Yeeles, 2018",N/A "VA61, C-terminal tagging of endogenous Ctf4: TTAAAAAAATTAATAATATAAGGGAAGCTAGATATGAACAGCAATTGAAACGTACGCTGCAGGTCGAC",This

(11) Wearable electrodriven switch actively delivers macromolecular drugs to fundus in non-invasive and controllable mannersNature communicationsJanuary 2, 2025Qin X, Shi H, Li H, Chu B, Zhang J, Wen Z, Sun X, Wang H, He Y.TAMRA-rDOSs The rDOSs were obtained by reacting the M13mp18 ssDNA scaffold (New England Biolabs, catalogue no. N4040S) with the short strand in 1 × TAE-Mg2+ buffer and annealing according to an established procedure, which

(12) A guide to building a low-cost centrifuge force microscope module for single-molecule force experimentsNature ProtocolsDecember 30, 2024Jibin Abraham Abraham Punnoose, Andrew Hayden, Chai S. Kam, Ken HalvorsenStreptavadin (Thermo Fisher Scientific, Cat. No. 65305)* M13mp18 single-stranded DNA (New England BioLabs, N4040S)** BtsCI enzyme (New England BioLabs, R0647S)** 10X CutSmart Buffer (New England BioLabs, included

(13) Viral DNA polymerase structures reveal mechanisms of antiviral drug resistanceCellOctober 3, 2024Sundaresh Shankar, Junhua Pan, Pan Yang, Yuemin Bian, Gábor Oroszlán, Zishuo Yu, Purba Mukherjee, David J. Filman, James M. Hogle, Mrinal Shekhar, et al.assays of WT and the W781V HSV PolΔN42 mutant were performed using a M13mp18 ssDNA template (NEB Cat# N4040S) with 5' 6-Carboxyfluorescein (6-FAM) labeled M13 forward primer (Integrated DNA Technologies) and were

(14) A lumen-tunable triangular DNA nanopore for molecular sensing and cross-membrane transportNature CommunicationsAugust 22, 2024Xiaoming Liu, Fengyu Liu, Hemani Chhabra, Christopher Maffeo, Zhuo Chen, Qiang Huang, Aleksei Aksimentiev, Tatsuo Araipowerful tools in biosensing and synthetic biology. Methods Materials M13mp18 single-stranded DNA (N4040S) was obtained from New England Biolabs. All oligonucleotides were purchased from Sangon Biotech. All

(15) Engineering psychrophilic polymerase for nanopore long-read sequencingFrontiers in Bioengineering and BiotechnologyJuly 1, 2024Yaping Sun, Danny Hsu Ko, Jie Gao, Kang Fu, Yuanchen Mao, Yun He, Hui TianRolling circle amplification (RCA) The template–primer complex was prepared by combining 200 nM of M13mp18 single-stranded DNA (N4040S, New England Biolabs, Cambridge, United Kingdom) and 1 μM of the primer. M13mp18 was a 7429-nt sscDNA

(16) Toward drift-free high-throughput nanoscopy through adaptive intersection maximizationScience AdvancesMay 24, 2024Hongqiang Ma, Maomao Chen, Phuong Nguyen, Yang Liucomponents included 10 nM M13 bacteriophage single-stranded DNA scaffold p7249 (New England BioLabs, N4040S), 250 nM folding staples, 250 nM biotinylated staples, and 250 nM DNA-PAINT docking strands. The mixture

(17) Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imagingCellMarch 28, 2024Schueder F, Rivera-Molina F, Su M, Marin Z, Kidd P, Rothman JE, Toomre D, Bewersdorf J.S1 Oligos conjugated to the OyOlink probe,AlphaThera,Table S4 M13mp18 scaffold,New England BioLabs,Cat#N4040S Recombinant DNA,Recombinant DNA,Recombinant DNA GFP-ManII,Rothman lab Zhang et al.18,N/A GFP-OMP25,de

(18) TBX15 facilitates malignant progression of glioma by transcriptional activation of TXDNC5iScienceFebruary 16, 2024Ge Y, Jia B, Zhang P, Chen B, Liu L, Shi Y, Huang S, Liu X, Wang R, Xie Y, et al.described previously. Briefly, a 7249-nucleotide circular single stranded scaffold DNA (M13mp18, 10nM, Cat.N4040S, New England Biolabs, England), staple strands (50nM each), and capture strands for FAoligo hybridization

(19) Supercoiling-dependent DNA binding: quantitative modeling and applications to bulk and single-molecule experimentsNucleic Acids ResearchJanuary 11, 2024Pauline J Kolbeck, Miloš Tišma, Brian T Analikwu, Willem Vanderlinden, Cees Dekker, Jan LipfertpUC19 (2.686 kbp) and M13mp18 (7.249 kbp), both obtained similar from NEB (catalogue numbers N3041S and N4040S, respectively) and purified with a PCR clean-up kit (Qiagen). DNA concentrations were determined using

(20) The separation pin distinguishes the pro- and anti-recombinogenic functions of Saccharomyces cerevisiae Srs2Nat CommunDecember 8, 2023Aviv Meir, Vivek B. Raina, Carly E. Rivera, Léa Marie, Lorraine S. Symington, Eric C. GreeneCat. No. BLU502A250UC). All reactions contained 2.5 µM (in nucleotides) of M13 ssDNA (NEB, Cat. No. N4040S), reactions were initiated by the addition of Srs2898 to a final concentration of 40 nM and reactions

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