Cell Staining Buffer from BioLegend

Product Specs
ItemCell Staining Buffer
CompanyBioLegend
Price $89.00Supplier Page View Company Product Page
Catalog Number420201
ApplicationsFC,ICC,ICFC
Quantity500ML

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Generation of hypoimmunogenic cardiomyocytes from human induced pluripotent stem cells with TAPBP knockoutiScienceNovember 20, 2026Yuki Yamamoto, Rei Ohno, Kayoko Shoda, Toshihiro Suzuki, Kuniko Momoi, Shohei Fujii, Yuka Kenmochi, Toshiaki Yoshikawa, Manami Shimomura, Nobuo Tsukamoto, et al.GENE,# 311-00404 Y-27632,FUJIFILM Wako Pure Chemical,# 034-24024 Cell Staining Buffer,BioLegend,# 420201 RIPA buffer,NACALAI TESQUE,# 08714 CHIR99021,FUJIFILM Wako Pure Chemical,# 034-23103 BMP4,R&D Systems,#

(2) Salicylic Acid as a Food-Derived Bioactive Constituent of Tiaoqi Daozhi Decoction: A Potential Functional Food Ingredient to Restoring Gut Barrier Function in Constipation-Predominant IBSFood Sci NutrOctober 26, 2026Min Duan, Lixia Huang, Yingyue Xu, Ya Huang, Xiaojun Yangobtain mononuclear cells. Cells were resuspended in flow cytometry staining buffer (BioLegend, Cat. No. 420201) and incubated with PE‐conjugated anti‐rat CD19 antibody (BioLegend, Cat. No. 202408, 1:200) for 30

(3) BCG vaccination mitigates tau pathology and restores cognitive function in PS19 miceSci AdvOctober 23, 2026Somnath Shee, Meixiang Huang, Meghraj Singh Baghel, Yuxin Zheng, Shichun Lun, Santosh K. Yadav, Nirbhay N. Yadav, Carlos E. Ruiz-Gonzalez, Sandeep Tyagi, Eric Nuermberger, et al.staining, cells were fixed with 4% paraformaldehyde solution, washed with cell staining buffer (BioLegend; 420201), filtered through strainer-capped BD FACS tubes (BD; 352235), and analyzed via flow cytometry. A total

(4) Astragalus Polysaccharides Alleviate Inflammatory Damage in Murine Rheumatoid Arthritis by Modulating Th17/Treg Balance via the Gut-Joint AxisMoleculesOctober 21, 2026Pu Yao, Jing Huang, Wenying Liu, Qian Yuan, Jun LvAntibody (FJK-16S), PE-Cyanine7 (25-5773-80, Invitrogen, Carlsbad, CA, USA), Cell Staining Buffer (420201, Biolegend, San Diego, CA, USA), TruStain FcX (anti-mouse CD16/32) (101319, Biolegend, San Diego, CA,

(5) Three high throughput compatible cell-based assays for identifying small molecule JAG1 upregulators for Alagille syndromeSLAS DiscoveryOctober 1, 2026Molly R Kulikauskas, Xiuli Huang, Ivan Pavlinov, Phillip Sanchez, Guibin Chen, Dvir Blivis, Ty Voss, Karsten Baumgaertel, Steve Rodems, Atena Farkhondeh, et al.95% humidity, 5 μL of antibodies diluted to 9x final concentration in blocking solution (BioLegend, #420201) was dispensed onto live cells using a BioRAPTR Flying Reagent Dispenser (Beckman Coulter): PE-conjugated

(6) Primordial germ cell-derived macrophages as a platform to study host–pathogen interactionsFrontiers in Cell and Developmental BiologySeptember 30, 2026Sudeepta Kumar Panda, Nima Dehdilani, Guillermo Tellez, Ricardo Corona Torres, Lorna Taylor, Charlotte Cody, Samer Halabi, Zhiguang Wu, Rachel Hawken, Mark P. Stevens, et al.centrifugation at 1,500 RPM for 5 min and washed twice with cell staining buffer (BioLegend, catalog number: 420201). Then, the cells were resuspended in 100 µL of Annexin V binding buffer (BioLegend, catalog number:

(7) Intracellular Staphylococcus aureus triggers isolate-specific host transcriptional responses alongside TNF-R1 regulated cell fate decisionsCell Commun SignalSeptember 28, 2026Annika Walter, Thorsten Bischler, Marvin Jungblut, Leonhard F. Breitsprecher, Filip Vilotic, Johanna Beck, Lucia Hofmann, Nicole Schäfer, Tanja Ziesmann, Rico Franzkoch, et al.A549/SaOS-2 cells, respectively, were sedimented (500 × g, 5 min), washed twice with cell staining buffer (#420201, BioLegend), and resuspended in 20 µl staining mix (18 µl PBS, 1 µl FITC-Annexin V, 1 µl 7-amino-actinomycin

(8) Physiological upregulation of RAG1 expression compromises the survival of CD4+CD8+ thymocytes in vitroFrontiers in ImmunologySeptember 23, 2026Abani Kanta Naik, Javid P. Mohammed, Rebecca A. Glynn, Craig H. Bassing, Michael S. Krangelcentrifugation at 1250 RPM for 6 min at 4°C, were washed once with 1 ml of cell staining buffer (Cat. No. 420201, BioLegend) and were resuspended in 1 ml of Annexin V binding buffer (Cat. No. 422201, BioLegend). 100μl

(9) Isolation of Human Umbilical Cord Blood Hematopoietic Stem Cells and Directed Differentiation Into MegakaryocytesBio ProtocSeptember 20, 2026Wenjun Xia, Zeqing Miao, Weiwei Zhang, Zhixia Liu, Huang Wu, Yahan Fan581) (BD Biosciences, catalog number: 555822) 21. Cell staining buffer (Biolegend, catalog number: 420201) 22. Trypan Blue stain, 0.4% (Gibco, catalog number: 15250-061) 23. 75% ethanol (LIRCON, 500 mL) 24.

(10) Dual Functions of B7-H3-MAPK Signaling Orchestrate Tumor Immunosuppression via Regulating the Plasticity of Myeloid-derived Suppressor Cells and FibroblastsCancer DiscovSeptember 18, 2026Wei Shi, Ping Xu, Qianlin Gu, Zhen Fan, Xin Liang, Yin Wang, Yuehui Zhao, Chenling Meng, Javier Leo, Justin J. Kim, et al.incubated at 4 °C for 1 hour on a rotator. After washing with the Cell Staining Buffer (BioLegend, 420201) three times, the fluorescence signals were determined using an Attune NxT Flow Cytometer (Thermo Scientific)

(11) Transcriptional landscape of direct reprogramming toward the hematopoietic lineageiScienceSeptember 18, 2026Jillian Cwycyshyn, Cooper Stansbury, Sarah Golts, Hyunsu Lee, Joshua Pickard, Walter Meixner, Indika Rajapakse, Lindsey A. Muirof cell staining buffer (BioLegend, #422301), pelleted, and resuspended in fresh buffer (BioLegend, #420201). Antibodies included APC anti-human CD34 (Clone 581, BioLegend #343509, RRID: AB_1877154), PE anti-human CD38

(12) Single-cell transcriptomics reveals critical role of CD74 in bone marrow-derived mesenchymal stem cellsStem Cells Transl MedSeptember 18, 2026Qinqin Xu, Carson Curtis, Jiahao Zhang, Arjun Verma, Aaron J Sheppard, Jeffrey Deng, Hicham Drissi, Shane Barton, Patrick Massey, Yufeng Dongcell number. Cell surface staining for flow cytometry Single-cell suspensions in Cell Staining Buffer (BioLegend #420201) were centrifuged at 350 g for 5 min. Viable cells were counted and resuspended in Cell Staining Buffer

(13) A lipoprotein-biomimetic nanoplatform for oncogenic KRAS mutation-specific lysosome-targeting protein degradationNature CommunicationsSeptember 17, 2026Hang Yang, Xinyi Zhou, Jiafeng Wang, Chenyue Yang, Yuzhao Jin, Xufeng Liao, Xiangrui Liu, Chuansheng Guo, Qian Xiao, Quan Zhou, et al.15 min and then incubated with TruStain FcX (101319, Biolegend) for 10 min in cell staining buffer (420201, Biolegend) and then stained with the appropriate antibodies on ice for 30 min. For intracellular cytokine

(14) Protocol for robust gene knockout and reliable validation in human cell lines using quad-guide RNA vectorsSTAR ProtocSeptember 16, 2026Michael Tobin, Jie Dai, Li-Chuan Chan, Weiwei GuoBio,Cat#: 631231 Lenti-x GoStix Plus,Takara Bio,Cat#: 631280 Cell Staining Buffer,Biolegend,Cat#: 420201 Polybrene Transfection Reagent,Millipore Sigma,Cat#: TR-1003-G Sterile Syringe Filters PES,Fisherbrand,Cat#

(15) Age-associated loss of lymphatic vessels promotes cardiac inflammationNature Cardiovascular ResearchSeptember 15, 2026Julian U. G. Wagner, Hamza Gulshan, Ibrahim Sultan, Salli Antila, Laia-Canes Esteve, David Rodriguez Morales, Mariano Ruz Jurado, David John, Emmanouil G. Solomonidis, Katja Schmitz, et al.pluriSelect) and centrifuged at 500g for 10 min. The pellets were resuspended in cell staining buffer (420201, BioLegend) and stained with the Zombie NIR viability dye (423105, BioLegend) and the following antibodies

(16) Mutant KRAS-m6A Epitranscriptome Axis Promotes Colorectal Cancer and is a Therapeutic TargetAdv Sci (Weinh)September 9, 2026Danyu Chen, Pingmei Huang, Ruizhi Tao, Fenfen Ji, Qinyao Wei, Lok Hin Ko, Lianxin Zhou, Huarong Chen, Alvin Cheung, Wei Kang, et al.stimulation. For surface target staining, the cells were resuspended in cell staining buffer (Biolegend; 420201) mixed with specific antibodies for 0.5 h at 4°C. The intracellular antigens were captured by specific

(17) Anti-CMV IgG titer determines organ-specific protection toward immune checkpoint blockade-induced toxicitiesJ Immunother CancerSeptember 7, 2026Gusztav Milotay, Martin Little, Sophie MacKay, Dylan Patrick Browne Muldoon, An-Nhi Huynh, Andrea Farkas, Shawn Sun, Guangyi Niu, Orion Tong, Chelsea A Taylor, et al.in online supplemental material. PBMCs were washed once in cell staining buffer (BioLegend, cat. no. 420201) and incubated with Streptavidin (BV785, BioLegend cat. no. 405249) for 30 min at 4°C for IgG4 staining.

(18) Recruited monocyte-derived macrophages drive T cell inflammation in immune checkpoint inhibitor-mediated pneumonitisCancer CommunicationsSeptember 2, 2026Xiaoran Cui, Renyong Zhi, Xiaoyan Li, Yinming Ji, Zhisong Hu, Pengfei Cui, Yuhui Qin, Tao Li, Liangliang Wu, Lingxiong Wang, et al.conditions, and the supernatant was discarded. The pellet was resuspended in 5 ml of Cell Staining Buffer (#420201, BioLegend) and then recentrifuged under identical parameters. After supernatant removal, the pellet

(19) Establishment of induced pluripotent stem cell line TRNDi045-A-38 carrying homozygous DOK7-related Congenital Myasthenia patient-mutation knock-in variant from parental KOLF2.1JStem Cell ResearchSeptember 1, 2026B. Malique Jones, Miao Xu, Jizhong Zou, Chengyu Liu, Yaqun Zou, Pankaj Pathak, Richa Madan Lomash, Rodica Stan, Carsten G. Bönnemann, Catherine Z. Chenwith 0.5% Triton-X-100 for 15 min and then cells were blocked with cell staining buffer (BioLegend, 420201) for 60 min. Primary antibodies were incubated overnight at 4° C in cell staining buffer. Secondary

(20) Pharmacological Inhibition of Small Extracellular Vesicle Secretion by ALK5i SD-208 via Lysosomal Rerouting of CD63+ CompartmentsJ Extracell VesiclesAugust 29, 2026Rahul Sanwlani, Georgina H. Thompson, Kyle Bramich, Ching-Seng Ang, Millie L. Trowbridge, Chris E. Duringer, Vlad Stolojan, Sean M. Davidson, Konstantinos Savvatis, John H. McVey, et al.BioLegend, 423109) for 30 min in the dark. For all other samples, 500 µL of Cell Staining Buffer (BioLegend, 420201) was added, followed by centrifugation at 400 × g for 5 min. Pellets were resuspended in 100 µL Cell Staining Buffer

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Cell Staining Buffer from BioLegend

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Cell Staining Buffer from BioLegend

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