Cell Staining Buffer from BioLegend

Product Specs
ItemCell Staining Buffer
CompanyBioLegend
Price $89.00Supplier Page View Company Product Page
Catalog Number420201
ApplicationsFC - Quality tested, ICC, ICFC - Reported in the literature, not verified in house
Quantity500 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) 30-hydroxygambogic acid activates overlapping and independent apoptotic pathways in HPV positive and HPV negative oral cancer cellsTumour Virus ResearchJune 1, 2026Danelle Grubbs, Sonia Whang, Valeria Rodarte, Briza Martinez, Valeri Filippov, John Chen, Jaqueline Coats, Julia Unternaehrer, Isaac Kremsky, Brigitte Vazquez, et al.of 200,000–300,000 cells per well. They were washed twice with Cell Staining Buffer (Biolegend, cat. 420201) before following the staining protocol for Annexin V (Biolegend, cat. 640918) and 7AAD (Biolegend,

(2) A comprehensive multi-omics and functional study of evolutionary adaptive responses to smokeiScienceMay 15, 2026Simon D. Pouwels, Hao Chen, Senani N.H. Rathnayake, Andy Lan, Rashad M. Mahbub, Anna Chi Ying Yeung, Corry-Anke Brandsma, Thamar J. Lobo, Victor Guryev, Irene H. Heijink, et al.medium for 20 h. Cells were collected and washed twice using Cell Staining Buffer (BioLegend; Cat. No. 420201) and subsequently centrifuged (1,200g, 5 min). Cells were resuspended and stained with 40:42 Annexin-V Binding Buffer

(3) Oral Administration of Withaferin A Increases Infiltration of CD4+ Helper T Cells in a Mouse Model of Mammary TumorJ Cancer PrevApril 30, 2026Eun-Ryeong Hahm, Krishna B. Singh, Shivendra V. Singh1,000 rpm for 3 minutes. Splenocytes were resuspended in cell staining buffer from BioLegend (cat. # 420201). For isolation of single cells from mammary tumors, tissues were homogenized by being minced with scalpel

(4) High altitude-mediated immune remodeling accelerates agingSci AdvApril 24, 2026Yu Xiao, Yongfu Li, Yaqi Mao, Zichong Jin, Mengjie Sun, Xiangyang Wang, Gan Zhao, Yihong Song, Jing Li, Zhiying Zhang, et al.counterstaining and dehydration before mounting. Flow cytometry Cells were incubated in buffer (BioLegend, 420201). For neutrophil staining within PBMCs, antibodies including CD45-PECy7 (BD Pharmingen, 552848), CD11b-FITC

(5) Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapyCell Reports MedicineApril 21, 2026Yanqin Liu, Jiliang Zhao, Kailu Yang, Qiongqiong Ma, Dongping Zhang, Lili Xu, Zhaoyuan Zhang, Zhongqian Yin, Jingru Chen, Yi Wang, et al.RP10611 Mouse M-CSF Recombinant Protein,Peprotech,Cat# 315-02-10UG Cell stain buffer,Biolegend,Cat# 420201 MPLAs Vaccigrade,InvivoGen,Cat# vac-mpls Recombinant Mouse IFN gamma Protein,Sino Biological,Cat#

(6) Engineered oncolytic virus armed with anti-PCSK9 scFv boosts long-term CD8+ T cell immunity via rewiring MHC-I antigen presentationCell Reports MedicineApril 21, 2026Huolun Feng, Yuhan Zhang, Zuda Huang, Jianlong Zhou, Yongfeng Liu, Xiao Xiao, Mingxi Chen, Xin Guo, Jiabin Zheng, Zejian Lyu, et al.Buffer,biosharp,Cat# BL504A RBC Lysis Buffer,Servicebio,Cat# G2015-500ML Cell Staining Buffer,BioLegend,Cat# 420201 TruStain FcX PLUS (anti-mouse CD16/32) Antibody,BioLegend,Cat# 156604 SDS-PAGE Protein Loading Buffer

(7) In Silico Perturbation Identifies Transcription Factors as Protective Targets in HSPCs After IrradiationInt J Mol SciApril 15, 2026Zongjian Tao, Qi Zhang, Yingying Chen, Shaoting Lv, Qilin Huang, Hongyue Tian, Qixiang Liu, Caihui Li, Yuyuan Wang, Hao Lu, et al.incubation for 30 min at 4 °C in the dark, the cells were washed and resuspended in cell staining buffer (420201; BioLegend, San Diego, CA, USA). The samples were analyzed using a BD FACSCanto™ II flow cytometer (BD

(8) Single-cell omics and flow cytometry identify distinct immune states of dural and brain-infiltrating IL-17-producing γδ T cells after experimental strokeJ NeuroinflammationApril 9, 2026Mingming Zha, Alina Jander, Haodi Cai, Marius Piepke, Karoline Degenhardt, Leo Winter, Tim Magnus, Mathias Gelderblommin at 4 °C protected from light. After three washes with cell staining buffer (BioLegend; Cat. No. 420201), cells were resuspended in PBS supplemented with 2% FBS. Propidium iodide (1:1000, Sigma Aldrich, Cat.

(9) Non-Glycosylated, Legumain-Cleavable ISACs Drive Potent Anti-Tumor Immunotherapy via a Bystander EffectMolecular Cancer TherapeuticsApril 2, 2026Anqi Zhang, Mohan Reddy Mullapudi, Lina Wu, Michele Yi, Mohammad Asikur Rahman, Aiman A. Yaseen, H. Kayla Zhong, Tracy A. Brooks, L. Nathan TumeyFollowing staining, samples were washed twice and resuspended in cell staining buffer (BioLegend, cat. #420201). Flow cytometric analysis was performed using a Bio-Rad ZE5 Cell Analyzer (RRID: SCR_019712), and data

(10) Intraperitoneal CF33-hNIS combined with PD-L1 blockade eradicates gastric cancer peritoneal metastases and prevents recurrence via durable T cell memoryJ Immunother CancerApril 1, 2026Annie Yang, Zhifang Zhang, Anthony K Park, Shyambabu Chaurasiya, Sang-In Kim, Jianming Lu, Yoya Vashi, Jennifer Cillis, Hannah Valencia, Courtney Chen, et al.(Biolegend, Catalog#400636, Clone#RTK4530), and washed with cell staining buffer (Biolegend, Catalog#420201). Stained cells were assessed with a BD LSRFortessa Flow Cytometer and analyzed using Flowjo software.

(11) Dose-dependent effects of mitoquinone on mitochondrial function and post-thaw quality of cryopreserved canine spermVeterinary WorldMarch 31, 2026Abbas Farshad, Emilia Diel, Axel Wehrenda marker. Initially, the sperm were washed twice with cold BioLegend cell staining buffer (Cat. No. 420201, BioLegend Inc., San Diego, CA, USA) and resuspended in annexin V-binding buffer (Cat. No. 422201, BioLegend Inc

(12) Systemic immune profiling uncovers divergent mechanisms and predictive biomarkers of response to combination immunotherapies in hepatocellular carcinomaJournal for ImmunoTherapy of CancerMarch 30, 2026Akira Nishio, Takahiro Kodama, Kazuma Daiku, Kazuki Maesaka, Satoshi Tanaka, Yasutoshi Nozaki, Tomohide Kurahashi, Kengo Matsumoto, Takatoshi Nawa, Seiichi Tawara, et al.Spike-in Guidance. Approximately 10 million PBMCs were resuspended in 45 µL of Cell Staining Buffer (Cat. 420201, BioLegend, USA), incubated with 5 µL of Human TruStain FcX (Cat. 422301) at 4°C for 10 min, followed

(13) Identification of STMN1 as a lactylation‑related driver of lung cancer progression using Mendelian randomizationMolecular Medicine ReportsMarch 30, 2026Yifan Cai, Yucheng Zhong, Honglin Wang, Shuang Zhu, Fang Huang, Qiuyue Zhang, Shaobo Huand the supernatant was discarded. The cell pellet was resuspended in 300 µl Stain Buffer (cat. no. 420201; Biolegend, Inc.), and PI-PE (cat. no. HY-D0815; MedChemExpress) and Annexin V-FITC (cat. no. 640949;

(14) Direct contact with natural killer cells reprograms monocyte-derived dendritic cells into a tolerogenic phenotypeImmunohorizonsMarch 26, 2026Ammar J Alsheikh, Liang Chen, Daniel Korenfeld, Jason Tam, Suresh Patil, Hsi-Ju Wei, Jianing Li, Suju Zhong, Feng Dong, Terry Melim, et al.min at room temperature. Then cells were spun down and washed with staining buffer (BioLegend, Cat#420201) and stained with antibody mix including anti-CD3 BV570 (BioLegend, Cat#300436), anti-CD4 cFluor YG384

(15) Protocol for generating human tonsil immune organoids with epithelial and germinal center layers using low-Matrigel cultureSTAR ProtocolsMarch 20, 2026Dandan Meng, Han Ding, Qiyu Deng, Xuqi Li, Yuan Tao, Xiao Liuwith your laser configuration. Commercial staining buffers (e.g., BioLegend Staining Buffer, Cat. No. 420201) can replace PBS + 2% FBS for consistent performance across replicates. Note: FMO controls are used

(16) Comprehensive single-cell metabolic profiling identifies targets to sensitize triple-negative breast cancer to chemo-immunotherapyCell Rep MedMarch 17, 2026Ying Xu, Xin-Yi Liu, Hang Zhang, Li Chen, Han Wang, Zhi-Ming Shao, Yi Xiao, Yi-Zhou JiangFixation Buffer,BioLegend,Cat# 420801 Cyto-Last™ Buffer,BioLegend,Cat# 422501 Cell Staining Buffer,BioLegend,Cat# 420201 Opal Polaris 7-Color Manual IHC Kit,AKOYA Biosciences,Cat# NEL861001KT Cell Counting Kit-8,Yeasen,Cat#

(17) Single-cell multi-omic analysis of mitochondrial mutational mosaicism and dynamicsNat CommunMarch 16, 2026Yu-Hsin Hsieh, Pauline Kautz, Lena Nitsch, Ambre M. Giguelay, Janet Liebold, Veronika Dimitrova, Stephania Contreras Castillo, Freya Jungen, Gabor Zsurka, Genevieve Trombly, et al.from BioLegend. Two million cells per cell line were resuspended in cell staining buffer (BioLegend, 420201) with Human TruStain FcX (BioLegend, 422301) for 10 min on ice. Cell lines were then stained with 0,25

(18) Overcoming claudin family homology: discovery of ARC101, a highly potent CLDN6-specific T-cell engager with a novel CD3 binder for ovarian adenocarcinomaMAbsMarch 10, 2026Danlin Yang, Neha Kamran, Gururaj Shivange, Kavita Kumari, Prabhu Srinivas Yavvari, Viduth K. Chaugule, Vishal Mahajan, Bridget Larkin, Scott R. Brodeur, Joe Erhardt, et al.collected in culture medium. Cells were centrifuged, resuspended in cell staining buffer (BioLegend, Cat# 420201), and plated at 2.0 × 105 cells per well in 96-well plates with a prepared Live/Dead (L/D) staining

(19) Dysregulated NK-cell gene expression defines the enduring symptoms of long COVID-19Front ImmunolMarch 9, 2026Urvi Ray, Antje Schulze Selting, Roshan Priyarangana Perera, Zhiqi Yang, Vladislav Lysenkov, Siri Göpel, Michael Bitzer, Madhuri S. Salker, Stephan Ossowski, Olaf Riess, et al.LEGENDplex™ SARS-CoV-2 Serological IgG Panel (3-plex) w/VbP,BioLegend,Cat#741132 Cell Staining Buffer,BioLegend,Cat#420201 N 184 DeNovix Acridine Orange/Propidium Iodide Assay,DeNovix,Cat#TN184 Trypan Blue,Sigma-Aldrich,Cat#T8154-100ML High

(20) A high-throughput, quantitative platform using 2D dissociated human cerebral organoids to model neuroinflammation in Alzheimer's diseaseNPJ DementMarch 9, 2026Meagan N. Olson, Nathaniel J. Barton, Luyao Feng, Samantha M. Chigas, Khanh Tran, Adrian R. Orszulak, Jafira M. Johnson, Pepper Dawes, Chandani Shrestha, Vishali R. Umaiyalan, et al.room temperature in the dark. Cells were then washed with 500 μl of cell staining buffer (BioLegend 420201). For intracellular antibodies, either 100 μl of 4% paraformaldehyde or 250 μl of Cytofix/Cytoperm fixation

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Cell Staining Buffer from BioLegend

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Cell Staining Buffer from BioLegend

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