A wide variety of enzymes are available for research applications in cellular and molecular biology. To narrow down your search, select desired filters or enter keywords in the provided text boxes. It may be helpful to note the enzyme source, reaction conditions and units of activity provided by the manufacturers before making a purchase.
T7 DNA Polymerase was originally isolated from the T7 Bacteriophage. Commercially, it is produced in E. coli consisting of two subunits: the 84-kDa T7 gene 5 polymerase and the E. coli thioredoxin cofactor that is necessary for polymerase processivity. Similar to the E. coli DNA Polymerase I, it has a 5’-3’ polymerase activity active at 37°C. It features a robust 3’-5’ exonuclease activity on both single and double stranded DNA that can be modified by the presence of EDTA. T7 DNA polymerase is recommended for gap filling and site-specific mutagenesis.