NF-κB p65 Mouse mAb From Cell Signaling Technology

August 09, 2023

Surgery
University of Texas Southwestern Medical Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

NF-κB p65 Mouse mAb

Catalog Number:

#6956

Clone Number:

L8F6

I used this antibody to check the expression of Nf-kB p65 in human colorectal cancer cell lines Colo205 and HT29. Primary incubation at 1:1000 at 4 degrees O/N worked really well for this antibody and gave a good signal at around 65 KDa.

Experimental Design and Results Summary

Applications

Western blot

Sample

Human colorectal cancer cell line Colo205 and HT29

Primary Incubation

1:1000, 4 degrees, O/N

Blocking Agent

5% non-fat milk

Secondary Incubation

1:20000, 1h at RT

Tertiary Incubation

None

Detection

Amersham ECL and picture taken using Chemidoc

Results Summary

The antibody detects a very clear band at 65 KDa and showed both Colo205 and HT29 cells to be positive expressing cells with HT29 showing a higher expression.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clear band, and no background.

The Bad

Cost is higher but comparatively, still reasonable.

The Bottom Line

Best p65 antibody used.

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