Integrated DNA Technologies
Alt-R® CRISPR-Cas9 tracrRNA, ATTO™ 550
1075927
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tracrRNA, ATTO™ 550 was used to form gRNA and RNP complex with Cas9 protein. After 24 hours of transfection. RNP-targeted cells were sorted by FACS to separate successfully transfected fluorescent cells.
RNP targeted cell sorting
RNP complex formation
Good electroporation method.
tracrRNA, ATTO™ 550 was used to form gRNA and RNP complex with Cas9 protein. After 24 hours of transfection. RNP-targeted cells were sorted by FACS to separate successfully transfected fluorescent cells. Results showed high knockout efficiency.
N/A
Enhance cas9 based knockout efficiency.
Fluorescence based cell sorting greatly enhance the knockout efficiency.