Great Hoechst 33342 Solution for Live Cell Staining

Babraham Institute

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Hoescht 33342

Catalog Number:

62249

Used this product at a final concentration of 1mg/ml, to stain the nuclei of living cells during imaging.

Experimental Design and Results Summary

Application

Live cell imaging

Starting Material

MCF10a cells

Tips

Have to dilute in modified PBS (8 mM sodium phosphate, 2 mM potassium phosphate, 140 mM sodium chloride, 10 mM potassium chloride, pH 7.4).

Results Summary

Great staining leading to clearly distinguishable MCF10a cells. Easy to use (just have to dilute) and stains easily within 15 minutes of addition to cells.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use.

The Bad

Quite expensive.

The Bottom Line

Great product - stains MCF10a cells within 15 minutes. Have to dilute in modified PBS (8 mM sodium phosphate, 2 mM potassium phosphate, 140 mM sodium chloride, 10 mM potassium chloride, pH 7.4).

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