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Apotracker Green
427402
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This reagent is used to identify cells undergoing apoptosis by staining for phosphatidylserine on the cell surface.
Flow cytometry
Cultured cells
Acquired in FITC channel
I cultured T cells in either plain media or media+DMSO to induced apoptosis. I then stained with Apotracker Green following manufacturer instructions. The DMSO-treated cells demonstrated increased fluorescence with a population of cells that did not yet undergo apoptosis.
N/A
Calcium-independent reagent that does not require use of special buffers.
None
I prefer this reagent to Annexin because it is compatible with any staining buffer.