Agilent 2100 Bioanalyzer
Automated Analysis System
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Powered by major advances in microfabrication
technology, the development
of a fully automated laboratory on a chip
is now a reality. Designed to integrate
sample preparation, fluid handling and
biochemical analysis, "lab-on-a-chip"
is revolutionizing life science research.
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Lab-on-a-chip technology uses
semiconductor-like micro-fabrication
techniques to translate experimental
and analytical protocols into chip
architectures comprising interconnected
fluid reservoirs and pathways.
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Electrokinetic or pressure forces
drive fluids in a controlled manner
through selected pathways. In this
way, it is possible to create the
functional equivalent of valves
and pumps capable of performing
manipulations such as dispensing,
mixing, incubation, reaction, sample
partition and detection.
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Lab-on-a-chip technology provides
scientists a more convenient and
productive way to gather and store
experimental data.
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Lab-on-a-chip technology provides:
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• Miniaturized fluid pathways give
shortened run times
• Microfabricated chips yield better
reproducibility than conventional
technologies
• Versatile chip design enables flexible
experiment design on one system
• Microscale format minimizes
sample and reagent consumption
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The Agilent lab-on-a-chip system includes:
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• Agilent 2100 bioanalyzer
• System and data review software
• Desktop or laptop PC
• Printer
• Accessories
• Start-up and operational services
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The Agilent 2100 bioanalyzer is
a unique analysis tool capable of
handling nucleic acids, proteins, and
cells on one platform. The specific
applicability to certain sample classes
is based on the use of kits that include
sample-specific reagents and chips.
LabChip® kits for a variety of RNA, DNA,
protein and cell assays are available.
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Developed together with Caliper
Technologies Corporation the Agilent
2100 bioanalyzer integrates:
• Sample handling
• Separation and detection
• Data analysis within a single
compact system
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Specifications
Weight
10 kg (22 lbs)
Dimensions (width x depth x height)
162 x 412 x 290 mm (6.4 x 16.2 x 11.4 in)
Line voltage
100-240 VAC
Line frequency
50-60 Hz
Power consumption
60 VA
Ambient operating temperature
5-40 °C (41-104 °F)
Safety standards: IEC, EN, CSA, UL
Installation Category II,
Pollution Degree 2
Laser Class 1 |
Molecular assays
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Automated quality control, sizing and
quanitation of RNA, DNA and proteins
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Pin-electrodes in the cartridge for
molecular assays are used to create
electrokinetic forces capable of driving
fluids – or molecules within fluids –
through micro-channels to perform
electrophoretic separations.
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Automated regulation of voltage or
current between these electrodes
controls the speed and direction of
fluid movement. The miniaturized fluid
pathways shorten run time, and the
strong electrokinetic driving forces
improve analyte resolution.
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Agilent offers a wide range of LabChip
kits for the analysis of RNA, DNA and
proteins. In conjunction with the
Agilent 2100 bioanalyzer, equipped with
electrode cartridge, these solutions
have many advantages over traditional
slab gel analysis:
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• Reduced manual labor
• Fast, accurate and reproducible results
• Automated data acquisition and analysis
• Digital data for easy archiving
•Minimized exposure to hazardous
material and reduced waste volume
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RNA solutions
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| Agilent offers RNA assays that allow
characterization of total or mRNA
samples fast, with unmatched sensitivity
and minimal sample consumption. |
The RNA 6000 Nano LabChip kit is the
industry standard for quality control
and quantitation of total and mRNA
samples for microarray experiments.
The RNA 6000 Pico kit delivers the
highest sensitivity for RNA quality
control to date, making it the tool
of choice for the analysis of RNA
from microdissected samples, needle
biopsies and first strand cDNA.
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High sensitivity with small sample volume
As little as 200 pg/µl RNA is required
per analysis. This saves you sample
for any following experiments.
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Catch RNA degradation right away
RNase degradation of total RNA is
easily detected by a shift in the RNA
size distribution towards smaller
fragments and a decrease in
fluorescence signal of
ribosomal peaks.
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Automated detection of
ribosomal RNA contamination
The Agilent 2100 bioanalyzer software
integrates the messenger RNA smear
and calculates the percentage of
ribosomal RNA contamination.
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DNA solutions
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| Optimization of PCR reactions
for sequencing, cloning and typing |
| Agilent's DNA assays allow you to
automatically size and quantitate PCR
fragments and restriction digests
accurately and reproducibly. |
PCR and RT-PCR are among the most
widely used techniques in molecular
biology. While in some cases, it is
sufficient to detect the presence or
absence of a PCR product, in many
cases quantitation of this product and
detection of unspecific amplification is
critical. The Agilent 2100 bioanalyzer
is the tool of choice for automated
sizing and quantitation of PCR and
RT-PCR products in single or multiplex
mode with unprecedented accuracy.
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| High sensitivity
Higher sensitivity, broad linear dynamic
range and sharpness of bands enables
the detection of less abundant products
that are not visible in an agarose gel
(e.g. non-specific amplifications or
carry-over products). |
| Improved sizing accuracy
Normalization to two internal
markers and a ladder provides
accurate and reproducible sizing. |
Automated and reproducible quantitation
The Agilent 2100 bioanalyzer software
quantitates each DNA fragment
automatically against internal
standards. This ensures increased
accuracy and reproducibility.
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Protein solutions
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Ensure successful protein
purification along each step
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Agilent’s protein assays can be used
to analyze cell lysates, column
fractions and purified proteins –
10 samples in less than 30 minutes.
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| Purifying a protein from crude extracts
or whole cell lysates is tedious and
involves many steps along which the
protein of interest can be lost.
The 2100 bioanalyzer and Protein
LabChip kits monitor the presence
of a protein during each purification
step, faster and less laborious than
with conventional SDS-PAGE. |
Be in control
Rapidly check the success of your
purification procedure during every
step of the process.
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| Significant time saving
From the time you press 'START'
see your first results after 7 minutes
and subsequent results at 2-minute
intervals. |
| No need for staining/destaining
During the run, samples are automatically
stained and destained,
avoiding manual steps required
with SDS-PAGE. |
Direct comparison of samples
The Protein LabChip kits confirm the
expression of a recombinant protein
in cell lysate samples. The software
overlay feature allows identification
of expressed recombinant proteins.
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No more gels to dry
The data is automatically stored
in digital format which can be
easily retrieved and shared.
This eliminates the manual
procedures of gel drying
and storage.
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Cell assays
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Simplified analysis of fluorescence
parameters of single cells
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| With lab-on-a-chip technology it is
possible to miniaturize assays and
instrumentation used to monitor cell
parameters. The controlled movement
of cells on the chip is generated by
pressure-driven flow. |
Agilent offers an add-on pressure
cartridge, cell fluorescence software and
a LabChip kit for multiple types of cell
assay applications. Combined with the
small-footprint Agilent 2100 bioanalyzer,
this makes performing simple flow
cytometric analyses on a chip a reality,
even for the smallest lab.
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Cell solutions
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The analysis of cell-based attributes is
a daily task in many molecular and cell
biology labs. A simple control method
is crucial for any analysis protocol used
for monitoring the expression of a target
protein, the transfection status of a cell
population, or the time course of an
apoptotic process.
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| Agilent’s cell assays can be used to
determine easily a variety of parameters
from many eukaryotic cell lines and
primary cells. |
| Low cell consumption
As little as 20,000 cells per sample are
loaded on the chip for analysis.
On-chip staining provides the added
benefit of minimal reagent and cell
consumption. |
| Ease of use
Extensively documented pre-defined
applications allow you to start right
away. These examples include
detection of antibody binding,
apoptosis monitoring or detection
of green fluorescent protein. |
Flexibility
The possibility to establish customdesigned
assays with the help of
easy software tools broadens the
applicability of the system.
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Software
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The software for molecular assays and
the software for cell assays provide easy
instrument control, access to real-time
data, automated data analysis and valuable tools for further data evaluation.
Free data review software for off-line
evaluation allows you to share data
with other scientists all over the world.
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The data organizer software is a useful
complement to the system software,
providing quick access to centrally stored
raw data files and analysis results for
review, re-evaluation or export.
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