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Assay Kit
Zombie NIR(TM) Fixable Viability Kit
Zombie NIR(TM) Fixable Viability Kit from BioLegend
(4)
Write a review
Citations:
(533)
Supplier Page
Supplier Page from
BioLegend for
Zombie NIR(TM) Fixable Viability Kit
Description
Zombie NIR(TM) Fixable Viability Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Zombie NIR(TM) Fixable Viability Kit
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
423106
Quantity
500 tests
Applications
Flow Cytometry (FC)
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (533)
(41)
A HER2-targeting antibody-MMAE conjugate RC48 sensitizes immunotherapy in HER2-positive colon cancer by triggering the cGAS-STING pathway
Cell death & disease
August 24, 2023
Wu X, Xu L, Li X, Zhou Y, Han X, Zhang W, Wang W, Guo W, Liu W, Xu Q,
et al
.
expression on the
cell membrane
. The TILs were stained with the following antibodies: Zombie NIRTM (
423105
,
Biolegend
) for dead cell preclusion,
CD45
-BV510 (
103138
,
Biolegend
), CD3-APC (
100236
,
Biolegend
), CD4-FITC
(42)
MyD88-dependent Toll-like receptor 2 signaling modulates macrophage activation on lysate-adsorbed Teflon™ AF surfaces in an in vitro biomaterial host response model
Frontiers in Immunology
August 24, 2023
Laura A. McKiel, Laurel L. Ballantyne, Gian Luca Negri, Kimberly A. Woodhouse, Lindsay E. Fitzpatrick
(TruStain fcX™; Biolegend) on ice for 10 minutes, followed by
incubation
with Zombie
NIR
® (cat. No.
423105
,
Biolegend
, San Diego, CA), 500 ng/ml of anti-mouse F4/80 (cat. No.
123115
,
Biolegend
) and 1.25 µg/mL
(43)
N-acetylglucosamine-6-O sulfation on intestinal mucins prevents obesity and intestinal inflammation by regulating gut microbiota
JCI Insight
August 22, 2023
Hirohito Abo, Aoi Muraki, Akihito Harusato, Tetsuya Imura, Maki Suzuki, Kohta Takahashi, Timothy L. Denning, Hiroto Kawashima
Bioscience. Dead cells were stained using the Zombie Aqua or Zombie
NIR
fixable dye
staining
kit (
423101
or
423105
,
BioLegend
). Intracellular
staining
was performed using an Intracellular Fixation and Permeabilization
(44)
Omicron infection following vaccination enhances a broad spectrum of immune responses dependent on infection history
Nature communications
August 21, 2023
Hornsby H, Nicols AR, Longet S, Liu C, Tomic A, Angyal A, Kronsteiner B, Tyerman JK, Tipton T, Zhang P,
et al
.
re-suspended in the residual volume (~50 μl). Samples were stained with Live/Dead Zombie
NIR
(
Biolegend
,
423106
) for 10 min at RT, after which and without washing, the surface antibody cocktail diluted in
Brilliant Stain Buffer
(45)
STING-IRG1 inhibits liver metastasis of colorectal cancer by regulating the polarization of tumor-associated macrophages
iScience
August 18, 2023
Liu Y, Sun Q, Zhang C, Ding M, Wang C, Zheng Q, Ma Z, Xu H, Zhou G, Wang X,
et al
.
AB_2136290
β-Actin Rabbit mAb
,
Abclonal
,Cat#
AC026
; RRID: AB_2768234 Zombie NIR™ Fixable Viability Kit,
Biolegend
,Cat#
423105
Brilliant
Violet
510™ anti-mouse CD45 Antibody,
Biolegend
,Cat#
103137
; RRID: AB_256139
FITC anti-mouse/human CD11b
(46)
Comparative immune responses to Mycobacterium tuberculosis in people with latent infection or sterilizing protection
iScience
August 18, 2023
Emilie Jalbert, Cuining Liu, Vidya Mave, Nancy Lang, Anju Kagal, Chhaya Valvi, Mandar Paradkar, Nikhil Gupte, Rahul Lokhande, Renu Bharadwaj,
et al.
APC-Fire750,
Biolegend
,"Cat#
301853
, RRID:AB_2632659" Zombie
NIR
Fixable Viability Dye,
Biolegend
,Cat#
423105
"MR1 BV421, 5-OP-RU",NIH Tetramer Core Facility,
CD56
BV480,
BD Biosciences
,"Cat#
566162
, RRID:AB_2739559"
CD8
(47)
Transplantation elicits a clonally diverse CD8+ T cell response that is comprised of potent CD43+ effectors
Cell Reports
August 16, 2023
Gregory S. Cohen, Melissa A. Kallarakal, Sahana Jayaraman, Francis I. Ibukun, Katherine P. Tong, Linda D. Orzolek, H. Benjamin Benjamin Larman, Scott M. Krummey
"
CellTrace Violet Cell Proliferation Kit, for flow cytometry
",
Invitrogen
,Cat#
C34557
Zombie NIR Fixable Viability Kit,
Biolegend
,Cat#
423106
Foxp3/Transcription Factor Staining Buffer Set,
Invitrogen
,Cat#
00-5523-00
Qiashredder
columns,
Qiagen
,Cat#
(48)
Development of a Patient-Derived 3D Immuno-Oncology Platform to Potentiate Immunotherapy Responses in Ascites-Derived Circulating Tumor Cells
Cancers (Basel)
August 16, 2023
Thomas J. Gerton, Allen Green, Marco Campisi, Minyue Chen, Iliana Gjeci, Navin Mahadevan, Catherine A. A. Lee, Ranjan Mishra, Ha V. Vo, Koji Haratani,
et al.
again. Afterwards, the samples were incubated in the dark with Zombie
NIR
viability dye (
Biolegend
,
423105
, Tokyo, Japan) resuspended in 1× PBS for 15 min. The cells were then washed again with 1× PBS supplemented
(49)
Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networks
Immunity
August 8, 2023
Ugur M, Labios RJ, Fenton C, Knöpper K, Jobin K, Imdahl F, Golda G, Hoh K, Grafen A, Kaisho T,
et al
.
1708890
RNeasy Plus Micro Kit,
QIAGEN
,Cat#
74034
Zombie NIR™ Fixable Viability Kit,
BioLegend
,Cat#
423106
Zombie Aqua™ Fixable Viability Kit,
BioLegend
,Cat#
423102
,, Deposited data,Deposited data,Deposited
(50)
Synergism Between IL21 and Anti-PD-1 Combination Therapy is Underpinned by the Coordinated Reprogramming of the Immune Cellular Network in the Tumor Microenvironment
Cancer Research Communications
August 4, 2023
Shaoxian Wu, Hao Huang, Runzi Sun, David Shihong Gao, Fan Ye, Jianing Huang, Ella Li, Andrew Ni, Kevin GuoKai Lu, Kong Chen,
et al.
RRID:AB_1279430) antibodies were purchased from
BioLegend
and BD Biosciences. Zombie
NIR
dye (catalog no.
423106
) was purchased from
BioLegend
. Ghost Dye Violet 510 was purchased from
Cell Signaling Technology
(catalog
(51)
Human galectin-9 potently enhances SARS-CoV-2 replication and inflammation in airway epithelial cells
Journal of Molecular Cell Biology
August 3, 2023
Li Du, Mohamed S Bouzidi, Akshay Gala, Fred Deiter, Jean-Noël Billaud, Stephen T Yeung, Prerna Dabral, Jing Jin, Graham Simmons, Zain Y Dossani,
et al.
E1500
).
Flow cytometry
Cells were detached with 10% EDTA containing Zombie
NIR
(
1:300
,
BioLegend
,
423105
) for 10 min at 37°C. Then, cells were washed three times with 1× PBS and incubated with
human
ACE2
Alexa-Fluor
(52)
Diversity of B Cell Populations and Immunoglobulin Repertoire in Human Lungs
The Journal of Immunology
August 1, 2023
Fumiaki Aihara, Yumei Wang, Anna C. Belkina, Rachel Fearns, Joseph P. Mizgerd, Feng Feng, Thomas B. Kepler
2H7), anti-
CD69
BV605 (
BioLegend
310938
,
Clone
FN50) and Zombie-NIR Fixable Viability Kit (
BioLegend
423106
). Cell samples were sorted with
BD
FACSARIA
II SORP into 1.5 ml Eppendorf tubes containing 600 μl of
(53)
An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression
Cell research
August 1, 2023
Wu L, Yan J, Bai Y, Chen F, Zou X, Xu J, Huang A, Hou L, Zhong Y, Jing Z,
et al
.
buffer (#
420201
,
Biolegend
, San Diego, CA, USA) and incubated with Zombie NIR™ Fixable Viability Kit (#
423106
,
Biolegend
),
PE anti-human CD45
antibody (#
368510
,
Biolegend
) and
PE/Cyanine7 anti-human CD86
antibody
(54)
SAP deficiency aggravates periodontitis possibly via C5a-C5aR signaling-mediated defective macrophage phagocytosis of Porphyromonas gingivalis
Journal of advanced research
August 1, 2023
Wang L, Liang D, Huang Y, Chen Y, Yang X, Huang Z, Jiang Y, Su H, Wang L, Pathak JL,
et al
.
times. Cells were counted and analyzed for cell viability using Zombie NIRTM Fixable Viability Kit (
423105
,
Biolegend
, Beijing, China). Cells were stained with 5 μg of PE-Cy7 anti-mouse F4/80 (
123114
, BioLegend,
(55)
Single cell RNA sequencing unravels mechanisms underlying senescence-like phenotypes of alveolar macrophages
iScience
July 21, 2023
Yue Wu, Shengen Shawn Hu, Ruixuan Zhang, Nick P. Goplen, Xiaochen Gao, Harish Narasimhan, Ao Shi, Yin Chen, Ying Li, Chongzhi Zang,
et al.
proteins","Chemicals, peptides, and recombinant proteins" ,, Zombie NIR™ Fixable Viability Kit,
Biolegend
,Cat#
423106
Zombie Aqua™ Fixable Viability Kit,
Biolegend
,Cat#
423102
Recombinant mouse GM-CSF,
Biolegend
,Cat#
576308
Recombinant
(56)
Preclinical evaluation of PHH-1V vaccine candidate against SARS-CoV-2 in non-human primates
iScience
July 21, 2023
Antoni Prenafeta, Gregori Bech-Sàbat, Alexandra Moros, Antonio Barreiro, Alex Fernández, Manuel Cañete, Mercè Roca, Luis González-González, Carme Garriga, Joachim Confais,
et al.
(Omicron),JPT peptide technologies,PM-SARS2-RBDMUT08-1 Zombie
NIR
fixable viability stain,
BioLegend
,Cat#
423105
Streptavidin PE-Cy7
conjugate,
eBioscience
,Cat#
25-4317-82
,, Critical commercial
assays
,Critical
(57)
PLGA Nanoparticle-Based Dissolving Microneedle Vaccine of Clostridium perfringens ε Toxin
Toxins (Basel)
July 19, 2023
Wei Wan, Yue Li, Jing Wang, Zhiying Jin, Wenwen Xin, Lin Kang, Junhong Wang, Xiaoyang Li, Yakun Cao, Hao Yang,
et al.
(
100752
, 53-6.7,
Biolegend
, San Diego, CA, USA); wash buffer (DPBS containing 1% FBS); live/dead dye (
423105
,
Biolegend
, USA); fixation solution; permeabilization washing buffer (
554714
,
BD Biosciences
, USA);
(58)
Chronic inflammation, neutrophil activity, and autoreactivity splits long COVID
Nature communications
July 14, 2023
Woodruff MC, Bonham KS, Anam FA, Walker TA, Faliti CE, Ishii Y, Kaminski CY, Ruunstrom MC, Cooper KR, Truong AD,
et al
.
washed in PBS, and resuspended in a PBS dilution of Zombie
NIR
fixable viability dye (
BioLegend
, no.
423106
). Cells were washed and fixed at 0.8% paraformaldehyde (PFA) for 10 min at 22 °C in the dark before
(59)
OX40L-Armed Oncolytic Virus Boosts T-cell Response and Remodels Tumor Microenvironment for Pancreatic Cancer Treatment
Theranostics
July 9, 2023
Shiyu Liu, Fan Li, Qiongqiong Ma, Mingjuan Du, Haoran Wang, Yiping Zhu, Li Deng, Wenrui Gao, Chunlei Wang, Yanqin Liu,
et al.
buffer (CS0001, Leagene). The single cell suspension was stained with Zombie NIR™ Fixable Viability Kit (
423105
,
BioLegend
) and immune cell markers (Table S1).
Flow cytometry analysis
was performed on an LSR Fortessa
(60)
Evolutionarily conserved amino acids in MHC-II mediate bat influenza A virus entry into human cells
PLOS Biology
July 6, 2023
Okikiola M. Olajide, Maria Kaukab Osman, Jonathan Robert, Susanne Kessler, Lina Kathrin Toews, Thiprampai Thamamongood, Jacques Neefjes, Antoni G. Wrobel, Martin Schwemmle, Kevin Ciminski,
et al.
catalog no.
550826
,
1:200
) for 30 min on ice. Zombie NIR Fixable Viability Kit (
BioLegend
, catalog no.
423105
,
1:1000
) was used to assess live versus dead status of cells. After a final wash and
centrifugation
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Reviews
Write a Review
(1)
11/29/2023
Reliable Zombie NIR™ Fixable Viability Kit From BioLegend
Varaang Bhandula
The Zombie NIR viability kit works well for identifying dead cells from cell suspensions isolated from mouse lungs, spleen, and lymph nodes. I recommend storing at -20C to ensure the dye works well ...
Read Review
(1)
4/24/2021
Relatively Good Fixable Live/Dead Dye
Hong Sun
We are interested in characterizing the immune populations in secondary lymphoid organs and tumors under different knockout condition.
(1)
1/11/2021
Using Zombie NIR to Detect Dead Cells
Alejandro Prados
We are using the Zombie NIR to characterize the immune cells populations in the mouse intestine during chronic inflammation. This reagent is perfect to clearly separate dead and live cells.
(1)
3/1/2016
Cell Viability Kit for FC
Maria Carmela Speranza
This is a good and easy to use cell viability staining kit for flow cytometry.
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Zombie NIR(TM) Fixable Viability Kit from BioLegend
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
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