Relatively Good Fixable Live/Dead Dye

April 24, 2021

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Zombie NIR™ Fixable Viability Kit

Catalog Number:

423106

Clone Number:

N/A

We are interested in characterizing the immune populations in secondary lymphoid organs and tumors under different knockout condition.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse MC38 Tumor

Primary Incubation

1:1000 in PBS for 30min RT

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Cytek Aurora

Results Summary

Able to distinguish live and dead cells

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good fixable dye to distinguish dead cells

The Bad

None

The Bottom Line

Able to distinguish live and dead cells and also allow for staining an extra marker in APC Cy7 family when using Cytek Aurora

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