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Assay Kit
Zombie NIR(TM) Fixable Viability Kit
Zombie NIR(TM) Fixable Viability Kit from BioLegend
(4)
Write a review
Citations:
(522)
Supplier Page
Supplier Page from
BioLegend for
Zombie NIR(TM) Fixable Viability Kit
Description
Zombie NIR(TM) Fixable Viability Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Zombie NIR(TM) Fixable Viability Kit
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
423105
Quantity
100 tests
Applications
Flow Cytometry (FC)
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (522)
(1)
Role of affinity in plasma cell development in the germinal center light zone
Journal of Experimental Medicine
January 1, 2024
Mohamed A. ElTanbouly, Victor Ramos, Andrew J. MacLean, Spencer T. Chen, Maximilian Loewe, Sandra Steinbach, Tarek Ben Tanfous, Brianna Johnson, Melissa Cipolla, Anna Gazumyan,
et al.
1A8-
Ly6g
,
47-9668-82
). Dead cells were identified and excluded using live/dead Zombie
NIR
(
BioLegend
,
423106
). For sorting DZ and LZ GC B cells sorting (Fig. 4), anti-Igλ1 (BD Biosciences,
clone
R26-46,
744526
),
(2)
Hepatitis D infection induces IFN-β-mediated NK cell activation and TRAIL-dependent cytotoxicity
Front Immunol
December 8, 2023
Christopher Groth, Jovana Maric, Irene Garcés Lázaro, Tomáš Hofman, Zhenfeng Zhang, Yi Ni, Franziska Keller, Isabelle Seufert, Maike Hofmann, Christoph Neumann-Haefelin,
et al.
130-059-901
). Live cells were identified using the Zombie NIR™ Fixable Viability Kit (
Biolegend
, Cat.:
423105
) Zombie Aqua™ Fixable Viability Kit (
Biolegend
, Cat.:
423101
), fixable viability dye e780 (eBioscience,
(3)
Protocol for isolation of signet ring cells from human gastric mucosa
STAR Protocols
December 1, 2023
Sarah G. Samaranayake, Lauren A. Gamble, Cassidy Bowden, Benjamin L. Green, Amber F. Gallanis, Dilara Akbulut, Niharika Shah, Jonathan M. Hernandez, Jeremy L. Davis
gastric mucosa
demonstrates a viable sample. 80.1% viability with Zombie Live-Dead
staining
(
BioLegend
Cat#
423105
) with significant cytokeratin expression via Alexa Fluor 647-conjugated pan-cytokeratin monoclonal antibody
(4)
Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenes
STAR Protocols
December 1, 2023
Yudai Xu, Zijian Wang, Wen Gao, Haoyun Wang, Zhixian Wang, Chenxuan Tian, Guobing Chen
7-AAD Viability Staining Solution
,
BioLegend
,Cat#
420404
Zombie NIR Fixable Viability Kit,
BioLegend
,Cat#
423105
Brain heart infusion (BHI),Oxoid,Cat# CM1135 Agar,Biosharp,Cat# BS195-1k
Cell Activation Cocktail (with Brefeldin A
(5)
RIPK3 promotes brain region-specific interferon signaling and restriction of tick-borne flavivirus infection
PLOS Pathogens
November 27, 2023
Marissa Lindman, Juan P. Angel, Irving Estevez, Nydia P. Chang, Tsui-Wen Chou, Micheal McCourt, Colm Atkins, Brian P. Daniels
clone
1A8), Ly6C (Biolegend,
clone
HK1.4), CD80 (Biolegend,
clone
16-10A1), and Zombie
NIR
(
Biolegend
, #
423105
).
Leukocytes
were stained for 30 minutes at 4C prior to washing in FACS buffer and fixation with 1%
(6)
Dominant CD4+ T cell receptors remain stable throughout antiretroviral therapy-mediated immune restoration in people with HIV
Cell Reports Medicine
November 21, 2023
Alexis Sponaugle, Ann Marie K. Weideman, Jolene Ranek, Gatphan Atassi, JoAnn Kuruc, Adaora A. Adimora, Nancie M. Archin, Cynthia Gay, Daniel R. Kuritzkes, David M. Margolis,
et al.
cultured for 5 days at 37°C. Cells were washed then stained with viability dye (Zombie
NIR
Biolegend
Cat #
423106
) and
CD3
AF488 (
Biolegend
Cat #
300415
), CD4 BV650 (
Biolegend
Cat #
317436
),
CD8
BV510 (
Biolegend
Cat
(7)
More time to kill: a longer liver stage increases T cell-mediated protection against pre-erythrocytic malaria
iScience
November 20, 2023
Naveen Yadav, Chaitra Parthiban, Zachary P. Billman, Brad C. Stone, Felicia N. Watson, Kevin Zhou, Tayla M. Olsen, Irene Cruz Cruz Talavera, Annette Mariko Seilie, Anya C. Kalata,
et al.
Cells were blocked with Fc CD16/32 (
clone
2.4G2, BD) containing
1:1000
Zombie viability dye (cat #
423105
,
BioLegend
) for 30 minutes. Surface
staining
was done by incubating cells for 20 minutes on ice with
(8)
Epidermal keratinocyte-specific STAT3 deficiency aggravated atopic dermatitis-like skin inflammation in mice through TSLP upregulation
Frontiers in Immunology
November 20, 2023
Zhao-Yuan Wang, Yu-Xin Zheng, Fan Xu, Ying-Zhe Cui, Xue-Yan Chen, Si-Qi Chen, Bing-Xi Yan, Yuan Zhou, Min Zheng, Xiao-Yong Man
(
1:100
, BioLegend, San Diego, CA), the cells were incubated with Live/Dead Zombie dye (UV,
BioLegend
,
423106
,
1:100
) and antibodies specific for
CD45
(BV510,
BioLegend
,
103138
,
1:100
) and FcϵRIα (PE/Cyanine7,
(9)
Early-to-mid stage idiopathic Parkinson’s disease shows enhanced cytotoxicity and differentiation in CD8 T-cells in females
Nature Communications
November 20, 2023
Christophe M. Capelle, Séverine Ciré, Fanny Hedin, Maxime Hansen, Lukas Pavelka, Kamil Grzyb, Dimitrios Kyriakis, Oliver Hunewald, Maria Konstantinou, Dominique Revets,
et al.
twice with PBS, PBMC were incubated with 100 μL of Zombie
NIR
Live/dead
staining
reagent (
Biolegend
,
423106
, dilution 1/200 in 1× PBS) for 30 min at RT. One hundred microliters of FCM buffer were added before
(10)
Ly6C-high monocytes alleviate brain injury in experimental subarachnoid hemorrhage in mice
Journal of Neuroinflammation
November 17, 2023
Huaijun Chen, Chaoran Xu, Hanhai Zeng, Zhihua Zhang, Ning Wang, Yinghan Guo, Yonghe Zheng, Siqi Xia, Hang Zhou, Xiaobo Yu,
et al.
antibody and assessing the live or dead status of the cells using with Zombie NIR™ Fixable Viability Kit (
423106
,
BioLegend
, USA), the cells were incubated with cell surface antibodies (Table 2) for 30 min at 4 ℃.
(11)
Immuno-metabolic dendritic cell vaccine signatures associate with overall survival in vaccinated melanoma patients
Nature Communications
November 8, 2023
Juraj Adamik, Paul V. Munson, Deena M. Maurer, Felix J. Hartmann, Sean C. Bendall, Rafael J. Argüello, Lisa H. Butterfield
combination of
Human
TureStain FcX (
Biolegend
,
422301
) and fluorescent cell viability dye (
Biolegend
,
423105
) for 10 min 4 °C in PBS. Following PBS wash step, primary antibodies against surface markers were incubated
(12)
Water Extract of Capsella bursa-pastoris Mitigates Doxorubicin-Induced Cardiotoxicity by Upregulating Antioxidant Enzymes
International Journal of Molecular Sciences
November 2, 2023
Yuhui Jeong, Sun-Ho Lee, Jangho Lee, Min-Sun Kim, Yu-Geon Lee, Jin-Taek Hwang, Sang-Yoon Choi, Ho-Geun Yoon, Tae-Gyu Lim, Seung-Hyun Lee,
et al.
24 h. Then, cells were washed with PBS and stained with Annexin V-FITC and Zombie NIRTM fixable dye (
423105
,
BioLegend
) for 15 min at RT (25 °C) in the dark. Apoptosis was measured by quantifying the population
(13)
Radiotherapy combined with docetaxel alters the immune phenotype of HNSCC cells and results in increased surface expression of CD137 and release of HMGB1 of specifically HPV-positive tumor cells
Neoplasia
November 1, 2023
Fridolin Grottker, Simon Gehre, Clara M. Reichardt, Azzaya Sengedorj, Tina Jost, Thorsten Rieckmann, Markus Hecht, Antoniu-Oreste Gostian, Benjamin Frey, Rainer Fietkau,
et al.
Diego, CA, USA" TNFRSF9/CD 137,BV 421,
311508
,
BioLegend
,"San Diego, CA, USA" Live/dead,Zombie
NIR
,
423105
,
BioLegend
,"San Diego, CA, USA" List of
flow cytometry
antibodies and dyes for immune checkpoint molecule
(14)
PD-L1-expressing cancer-associated fibroblasts induce tumor immunosuppression and contribute to poor clinical outcome in esophageal cancer
Cancer immunology, immunotherapy : CII
November 1, 2023
Kawasaki K, Noma K, Kato T, Ohara T, Tanabe S, Takeda Y, Matsumoto H, Nishimura S, Kunitomo T, Akai M,
et al
.
were also used. Dead cells (
1:1000
dilution) were stained using a Zombie NIR Fixable Viability Kit (
423106
,
BioLegend
). Stained cells were analyzed using
flow cytometry
(FACSLyric; BD Biosciences, Franklin Lakes,
(15)
Optimization of ionizable lipids for aerosolizable mRNA lipid nanoparticles
Bioengineering & Translational Medicine
November 1, 2023
Mae M. Lewis, Melissa R. Soto, Esther Y. Maier, Steven D. Wulfe, Sandy Bakheet, Hannah Obregon, Debadyuti Ghosh
resuspended in 50 μL 1× PBS for
staining
. Each sample was stained with 1 μL of
1:10
dilution of Zombie
NIR
(
423105
,
BioLegend
) and incubated at 4°C for 30 min. Cells were then washed with 1 mL of 1× PBS twice, resuspended
(16)
PKR-mediated stress response enhances dengue and Zika virus replication
mBio
October 31, 2023
Taissa Ricciardi-Jorge, Edroaldo Lummertz da Rocha, Edgar Gonzalez-Kozlova, Gabriela Flavia Rodrigues-Luiz, Brian J. Ferguson, Trevor Sweeney, Nerea Irigoyen, Daniel Santos Mansur
GADD34 antibody
(Proteintech;
10449-1-AP
, dilution
1:250
[IF/FACS]); Zombie
NIR
fixable viability dye (
BioLegend
;
423105
, dilution
1:750
); goat anti-rabbit IgG (H+L) Alexa Fluor 647 conjugated (
Invitrogen
;
A-21245
, dilution
(17)
Lung cDC1 and cDC2 dendritic cells priming naive CD8+ T cells in situ prior to migration to draining lymph nodes
Cell Reports
October 31, 2023
Youhui Si, Yihan Wang, Qiaomu Tian, Qiang Wang, Jared M. Pollard, Pramod K. Srivastava, Aaron P. Esser-Kahn, Joel H. Collier, Anne I. Sperling, Anita S. Chong
commercial
assays
,Critical commercial
assays
,, Zombie NIR™ Fixable Viability Kit,
BioLegend
,Cat#
423105
UltraComp eBeads™
Compensation Beads
,Invitrogen,Cat# 501129040 CellTrace CFSE Cell Proliferation Kit,
Invitrogen
,Cat#
(18)
CXCL13 promotes thermogenesis in mice via recruitment of M2 macrophage and inhibition of inflammation in brown adipose tissue
Frontiers in Immunology
October 23, 2023
Lijun Xie, Huiying Wang, Dan Wu, Feng Zhang, Wei Chen, Yuqing Ye, Fang Hu
Flow cytometry
Single cells of SVF fraction were resuspended with PBS and stained with zombie
NIR
™ dye (#
423105
,
Biolegend
, USA), blocked with FcX Blocking CD16/32 (#
156604
), and dyed with a mix of surface marker
(19)
Interferon-γ couples CD8+ T cell avidity and differentiation during infection
Nature Communications
October 23, 2023
Lion F. K. Uhl, Han Cai, Sophia L. Oram, Jagdish N. Mahale, Andrew J. MacLean, Julie M. Mazet, Theo Piccirilli, Alexander J. He, Doreen Lau, Tim Elliott,
et al.
staining
and surface
staining
was performed using Zombie NIR Fixable Viability Kit (
Biolegend
, #
423106
/
423105
), TruStain FcXTM (anti-mouse CD16/32,
Biolegend
, #
101319
) and fluorochrome-conjugated primary antibodies
(20)
Tumor cell-induced platelet aggregation accelerates hematogenous metastasis of malignant melanoma by triggering macrophage recruitment
Journal of experimental & clinical cancer research : CR
October 23, 2023
Chen Y, Zhou J, Liu Z, Wu T, Li S, Zhang Y, Yin X, Yang G, Zhang G.
(
BioLegend
, catalog no.
426102
, USA)], and Zombie NIR Fixable Viability Kit (
BioLegend
, catalog no.
423105
, USA). The RedFluor™ 710 Anti-Mouse CD45 (30-F-11) (TONBO Biosciences, catalog no. 80–0451, USA),
PerCP-Cyanine5.5 anti-Mouse F4/80 antigen (BM8.1
Page
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Reviews
Write a Review
(1)
2/16/2021
Excellent Viability Stain for Flow Cytometry
Vincent Giacalone
Neutrophils were isolated from blood, migrated through a transwell, and stained for viability in addition to surface markers.
(1)
1/8/2018
Good Live/Dead Discrimination On PBMCs
Dejan Mesner
The aim was to discriminate between live and dead cells for further downstream analysis. Human CD4+ T cells isolated from PBMCs were used in the experiment. I chose this product as Biolegend offers ...
Read Review
(1)
3/1/2016
Cell Viability Kit for FC
Maria Carmela Speranza
This is a good and easy to use cell viability staining kit for flow cytometry.
(1)
5/16/2014
Great Viability Dye
Maigan Hulme
I am using this product to gate out dead cells for intracellular transcription factor staining in mouse lymphocytes.
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<
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Zombie NIR(TM) Fixable Viability Kit from BioLegend
Supplier Page
View Company Product Page
Pricing Info
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Company Info
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