BioLegend
Zombie NIR™ Fixable Viability Kit
423105
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Neutrophils were isolated from blood, migrated through a transwell, and stained for viability in addition to surface markers
Flow cytometry
Blood neutrophils
No changes from manufacturer’s protocol
After migration through a transwell, neutrophils were stained for viability and surface markers. Dead cells are clearly separated from live cells by retaining this amine-reactive stain and are much brighter than live cells
N/A
Works well for any cell type, spillover into other red channels is reasonable
None for this stain
This is currently my most reliable and effective viability stain for flow cytometry