His Tag Protein Purification

His tag purification kits and reagents are designed for the straightforward and optimized extraction of recombinant proteins with polyhistidine tags. Also known as 6-His, the short hexapeptide tag features a high affinity for the metals, nickel or copper, and can be attached to the N- or C-terminus of a protein. A common method of His tag purification, immobilized metal affinity chromatography (IMAC), utilizes metal ligands bound to a stationary solid support, such as agarose. Kits that use magnetic beads are also commonly used, allowing for rapid isolations. In addition to the desired scale or throughput of the purification, also consider the expression system when choosing a kit to purify His-tagged proteins.