Oligo(dT)15 Primer from Promega

Product Specs
ItemOligo(dT)15 Primer
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC1101
TargetPoly A
Quantity20microg
Conjugate/Tag/LabelUnconjugated
Primer TypeOligo dT Primers

Sequence

Oligo(dT)-15

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Plasma level of omentin-1, its expression, and its regulation by gonadotropin-releasing hormone and gonadotropins in porcine anterior pituitary cellsScientific ReportsNovember 7, 2023Natalia Respekta, Karolina Pich, Ewa Mlyczyńska, Kamil Dobrzyń, Christelle Ramé, Tadeusz Kamiński, Nina Smolińska, Joëlle Dupont, Agnieszka Rakribonuclease inhibitor, and Moloney murine leukemia virus reverse transcriptase (cat. no. U1515, A3561, C1101, N2515, and M1705, respectively, Promega, USA). Specific primers for ITLN1 and the reference genes PPIA

(2) Jaw Bone Invasion of Oral Squamous Cell Carcinoma Is Associated with Osteoclast Count and Expression of Its Regulating Proteins in Patients and OrganoidsJournal of clinical medicineSeptember 18, 2023de Kort WWB, Haakma WE, van Es RJJ, Gawlitta D, Driehuis E, Gansevoort M, Willems SM.on ice, and a volume equal to 500 ng was incubated with 50 μg/mL Oligo (dT) 15 Primer (catalog no. C1101, Promega, Madison, WI, USA) in water for 5 min at 70 °C. To generate cDNA, GoScript Reverse Transcriptase

(3) Eicosapentaenoic Acid Induces the Inhibition of Adipogenesis by Reducing the Effect of PPARγ Activator and Mediating PKA Activation and Increased COX-2 Expression in 3T3-L1 Cells at the Differentiation StageLife (Basel)August 8, 2023Michael N. N. Nartey, Hidehisa Shimizu, Hikaru Sugiyama, Manami Higa, Pinky Karim Syeda, Kohji Nishimura, Mitsuo Jisaka, Kazushige Yokota48 h after the differentiation stage and on days 6 of the maturation stage. Using oligo-(dT)15 (Cat: C1101) and a random 9-mer (Cat: C1181) (Promega Corp., Madison, WI, USA) as primers in the RT process, single-stranded

(4) GDF10 is related to obesity as an adipokine derived from subcutaneous adipose tissueFront Endocrinol (Lausanne)July 14, 2023Mi Kyung Song, Ji Eun Kim, Jung Tae Kim, Yea Eun Kang, Sun Jong Han, Seok Hwan Kim, Hyun Jin Kim, Bon Jeong Ku, Ju Hee LeeWaltham, MA, USA). Complementary DNA (cDNA) was synthesized from the RNA (1 μg) using Oligo(dT)15 primer (C1101, Promega, Madison, WI, USA) and M-MLV reverse transcriptase (28025, Thermo Fisher Scientific). The produced

(5) FBXL4 ubiquitin ligase deficiency promotes mitophagy by elevating NIX levelsThe EMBO journalJuly 3, 2023Elcocks H, Brazel AJ, McCarron KR, Kaulich M, Husnjak K, Mortiboys H, Clague MJ, Urbé S.transcription (Thermo Scientific, 11541515) with RNasin (Promega, N251S), oligo (dT) 15 primer (Promega, C1101) and PCR nucleotide mix (Promega, U144B). Quantitative PCRs were performed in triplicate using primers

(6) Cohesin SMC1β promotes closed chromatin and controls TERRA expression at spermatocyte telomeresLife science allianceJuly 1, 2023Biswas U, Deb Mallik T, Pschirer J, Lesche M, Sameith K, Jessberger R.reverse transcribed to cDNA using the Promega cDNA kit (M3682; Promega), together with oligo(dT)15 (C1101; Promega). To amplify UUAGGG repeat transcripts, we used CCCTAA-specific oligos for generation of cDNA

(7) The transcriptional regulator Sin3A balances IL‐17A and Foxp3 expression in primary CD4 T cellsEMBO reportsMay 4, 2023Laura Perucho, Laura Icardi, Elisabetta Di Simone, Veronica Basso, Alessandra Agresti, Amaia Vilas Zornoza, Teresa Lozano, Felipe Prosper, Juan José Lasarte, Anna Mondinoretro transcriptase M‐MLV (Invitrogen, #28025‐013) in the presence of oligo(dT)15 Primer (Promega, #C1101), dNTPs (Euroclone), and RNAsin Ribonuclease Inhibitor (Promega, #N2111). The cDNA was diluted 10 times

(8) Primary cilia sense glutamine availability and respond via asparagine synthetaseNature metabolismMarch 1, 2023Steidl ME, Nigro EA, Nielsen AK, Pagliarini R, Cassina L, Lampis M, Podrini C, Chiaravalli M, Mannella V, Distefano G, et al.Complementary DNA was obtained by reverse transcription of extracted RNA using Oligo(dT)15 primers (Promega, #C1101) or Random Primers (Promega, #C1181) and ImProm-II Reverse Transcriptase (Promega, #A3802). Quantitative real-time PCR

(9) Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine EndometriumInternational Journal of Molecular SciencesFebruary 10, 2023Ana Amaral, Nélio Cebola, Anna Szóstek-Mioduchowska, Maria Rosa Rebordão, Paweł Kordowitzki, Dariusz Skarzynski, Graça Ferreira-DiasPromega, Madison, WI, USA) from 1 µg of total RNA in a 20 µL reaction volume, using an oligo (dT) primer (C1101; Promega, Madison, WI, USA). The primer sequences for target gene COL1A2 and reference gene ribosomal

(10) Hepatic PTP4A1 ameliorates high-fat diet-induced hepatosteatosis and hyperglycemia by the activation of the CREBH/FGF21 axisTheranosticsJanuary 22, 2023Byungtae Hwang, Min-Gi Kwon, Min Ji Cho, Nam-Kyung Lee, Jangwook Lee, Jeong Woong Lee, Kyoung-Jin Oh, Kwang-Hee Bae, Jung Hwan Hwang, Jeong-Ki Min, et al.Complementary DNA was synthesized from target RNA using the M-MLV Reverse Transcriptase kit (N1705, C1101, N2515, U1518; Promega). The qRT-PCR was performed using the quantiMix SYBR kit (QS105; PKT Co.). The

(11) Vitamin D Receptor (VDR) Genetic Variants: Relationship of FokI Genotypes with VDR Expression and Clinical Disease Activity in Systemic Lupus Erythematosus PatientsGenes (Basel)November 3, 2022Mónica R. Meza-Meza, Barbara Vizmanos, Melissa Rivera-Escoto, Adolfo I. Ruiz-Ballesteros, Karen Pesqueda-Cendejas, Isela Parra-Rojas, Margarita Montoya-Buelna, Sonia Luquín, Bertha Campos-López, Paulina E. Mora-García, et al.the cDNA synthesis. The cDNA synthesis was performed with oligo (dT) primers (Oligo (dT)15 Primer, C1101, PROMEGA Corporation, Fitchburg, WI, USA). The mRNA expression of VDR was determined by the TaqmanTM

(12) Stimulus-specific remodeling of the neuronal transcriptome through nuclear intron-retaining transcriptsThe EMBO journalNovember 2, 2022Mazille M, Buczak K, Scheiffele P, Mauger O.SuperScript III reverse transcriptase (Thermo Fisher, 18080044), dNTPs (Sigma, D7295) and oligo(dT)15 primer (Promega, C1101). PCR Semi‐quantitative PCR was performed using Phusion High‐Fidelity DNA Polymerase (New England Biolabs

(13) Modulation of receptor-like transmembrane kinase 1 nuclear localization by DA1 peptidases in ArabidopsisProceedings of the National Academy of SciencesOctober 4, 2022Benguo Gu, Hui Dong, Caroline Smith, Guicai Cui, Yunhai Li, Michael W. BevanSuperScript IV Reverse Transcriptase (Life Technologies Ltd 18090050) with Oligo(dT)15 Primer (Promega C1101). Quantitative reverse transcription–PCR reactions (qRT-PCR) used Lightcycler 480 SYBR Green I Master

(14) Resistance to tyrosine kinase inhibitors promotes renal cancer progression through MCPIP1 tumor-suppressor downregulation and c-Met activationCell death & diseaseSeptember 22, 2022Marona P, Górka J, Kwapisz O, Jura J, Rys J, Hoffman RM, Miekus K.performed using 1 mg of total RNA, oligo(dT) 15 primer (1 μg/μl, Promega, Madison, WI, USA, cat. no. C1101), dNTPs (10 mM, Promega, cat. no. U1330) and M-MLV reverse transcriptase (Promega, cat. no. M1701).

(15) Optimized human intestinal organoid model reveals interleukin-22-dependency of paneth cell formationCell stem cellSeptember 1, 2022He GW, Lin L, DeMartino J, Zheng X, Staliarova N, Dayton T, Begthel H, van de Wetering WJ, Bodewes E, van Zon J, et al.U",Promega,Cat#N2515 GoScript™ Reverse Transcriptase kit,Promega,Cat#A5000 "Oligo(dT) 15 Primer, 500ug/ml 20ug",Promega,Cat#C1101 "Random Primer, 500ug/ml 20ug",Promega,Cat#C1181 AgeI-HF,NEB,Cat#R3552L BbsI-HF,NEB,Cat#R3539S RNeasy Mini Kit (250

(16) Endocytic membrane repair by ESCRT-III controls antigen export to the cytosol during antigen cross-presentationCell reportsAugust 16, 2022Gros M, Segura E, Rookhuizen DC, Baudon B, Heurtebise-Chrétien S, Burgdorf N, Maurin M, Kapp EA, Simpson RJ, Kozik P, et al.of 20 μL using the MAXIMA retrotranscriptase (#EP0741, ThermoFisher) in combination with oligo dT (#C1101, Promega), RNase inhibitor (#N2511, Promega) and dNTPs (Promega), following manufacturer’s instructions.

(17) KLHL3 deficiency in mice ameliorates obesity, insulin resistance, and nonalcoholic fatty liver disease by regulating energy expenditureExperimental & molecular medicineAugust 1, 2022Jang JH, Lee JW, Cho MJ, Hwang B, Kwon MG, Kim DH, Lee NK, Lee J, Park YJ, Yang YR, et al.Life Technologies). First-strand cDNA was synthesized using an M-MLV Reverse Transcriptase kit (N1705, C1101, N2515, U1518; Promega) following the manufacturer’s instructions. The cDNA was used as a template for

(18) CROWN ROOTLESS1 binds DNA with a relaxed specificity and activates OsROP and OsbHLH044 genes involved in crown root formation in riceThe Plant JournalJuly 9, 2022Mathieu Gonin, Kwanho Jeong, Yoan Coudert, Jeremy Lavarenne, Giang Thi Hoang, Martine Bes, Huong Thi Mai To, Marieâ€Rose Ndella Thiaw, Toan Van Do, Daniel Moukouanga, et al.transcriptase kit (Qiagen 205111, Qiagen, Venlo, the Netherlands) with oligo(dT)15 primers (Promega C1101, Promega, Madison, WI, USA). The relative transcript abundance of selected genes was determined using

(19) Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibitionNeuronJuly 6, 2022Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, Bischofberger J, Scheiffele P.total RNA was reverse transcribed using random hexamers (Promega C1181) or Oligo(dT)15 primer (Promega C1101) for flanking primer analysis and ImProm II reverse transcriptase (Promega A3802). For qPCR assays,

(20) 1,25‐Dihydroxyvitamin D3 suppresses CD4+ T‐cell effector functionality by inhibition of glycolysisImmunologyJuly 1, 2022Emma L. Bishop, Nancy H. Gudgeon, Gillian M. Mackie, Daniel Chauss, Jennie Roberts, Daniel A. Tennant, Kendle M. Maslowski, Behdad Afzali, Martin Hewison, Sarah DimeloeM-MLV Reverse Transcriptase (Cat# M1701), RNasin Plus Ribonuclease Inhibitor (Cat# N2611), oligo(dT) 15 Primer (Cat# C1101), and PCR Nucleotide Mix (Cat# C1141). SYBR Green PCR Master Mix (Applied Biosystems, Cat# 4309155)

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