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Polymerase Chain Reaction (PCR)
Primers and Probes
Oligo(dT)15 Primer
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Oligo(dT)15 Primer
Oligo(dT)15 Primer
Oligo(dT)15 Primer from Promega
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Citations:
(118)
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Oligo(dT)15 Primer
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Description
Use for first-strand cDNA synthesis with a reverse transcriptase
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Oligo(dT)15 Primer
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
C1101
Target
Poly A
Quantity
20microg
Conjugate/Tag/Label
Unconjugated
Primer Type
Oligo dT Primers
Sequence
Oligo(dT)-15
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (118)
(21)
1,25‐Dihydroxyvitamin D3 suppresses CD4+ T‐cell effector functionality by inhibition of glycolysis
Immunology
July 1, 2022
Emma L. Bishop, Nancy H. Gudgeon, Gillian M. Mackie, Daniel Chauss, Jennie Roberts, Daniel A. Tennant, Kendle M. Maslowski, Behdad Afzali, Martin Hewison, Sarah Dimeloe
M-MLV Reverse Transcriptase
(Cat#
M1701
), RNasin Plus
Ribonuclease Inhibitor
(Cat#
N2611
),
oligo(dT) 15 Primer
(Cat#
C1101
), and
PCR Nucleotide Mix
(Cat#
C1141
).
SYBR Green PCR Master Mix
(
Applied Biosystems
, Cat#
4309155
)
(22)
AZFa Y gene, DDX3Y, evolved novel testis transcript variants in primates with proximal 3´UTR polyadenylation for germ cell specific translation
Scientific Reports
May 27, 2022
P. H. Vogt, M-A. Rauschendorf, J. Zimmer, C. Drummer, R. Behr
from the total RNA samples was performed after
incubation
with
Oligo(dT)15 primer
(
Promega
; cat. no.
C1101
) by reverse transcription (RT) with the
Promega
M-MLV Reverse Transcriptase
enzyme (Cat. No.
M3683
)
(23)
PHGDH is required for germinal center formation and is a therapeutic target in MYC-driven lymphoma
The Journal of clinical investigation
May 2, 2022
D'Avola A, Legrave N, Tajan M, Chakravarty P, Shearer RL, King HW, Kluckova K, Cheung EC, Clear AJ, Gunawan AS,
et al
.
according to the manufacturer’s instructions. RNA was reverse transcribed using oligo(dT) primers (
Promega
,
C1101
) and M-MLV (
Promega
,
M1701
) in the presence of RNase inhibitor (
Promega
,
N2511
).
Quantitative PCR
(
qPCR
)
(24)
Environmental control of Pub1 (NEDD4 family E3 ligase) in Schizosaccharomyces pombe is regulated by TORC2 and Gsk3
Life science alliance
May 1, 2022
Wang T, Woodman P, Humphrey SJ, Petersen J.
M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant
(Cat. no.
M3683
;
Promega
). DNAse-treated RNA, 500 ng Oligo (DT)15 (Cat. no.
C1101
;
Promega
) and 100 ng random hexamer (Cat. no.
C1181
;
Promega
) and heated to 70°C for 5 min, cooled to
(25)
The
Candida albicans
toxin candidalysin mediates distinct epithelial inflammatory responses through p38 and EGFR-ERK pathways
Science Signaling
April 5, 2022
Spyridoula-Angeliki Nikou, Chunsheng Zhou, James S. Griffiths, Natalia K. Kotowicz, Bianca M. Coleman, Mary J. Green, David L. Moyes, Sarah L. Gaffen, Julian R. Naglik, Peter J. Parker
NanoDrop 100 spectrophotometer.
First-strand cDNA synthesis
was performed using oligo(dt)15 primers (#
C1101
,
Promega
) and nuclease-free water under 70°C for 5 min. Reverse transcription was performed using the
(26)
Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions
International Journal of Molecular Sciences
March 8, 2022
Karine Pinel et al.
RNAseH reverse transcriptase kit (#18080-093, Invitrogen, Carlsbad, CA, USA) with random hexamers (#
C1101
,
Promega
, Madison, WI, USA). For all genes analyzed, the reverse transcription (RT) reactions were carried
(27)
Dysregulation of Receptor for Advanced Glycation End Products (RAGE) Expression as a Biomarker of Keratoconus
Disease Markers
January 15, 2022
Valentin Navel, Jean Malecaze, Corinne Belville, Héléna Choltus, Fanny Henrioux, Frédéric Dutheil, François Malecaze, Frédéric Chiambaretta, Loïc Blanchon, Vincent Sapin
Denovix
DS-11 FX
NanoDrop. The cDNA was synthetized from 1 μg of RNA using oligo-(dT) primers (
Promega
,
C1101
), 10 mM dNTP (Invitrogen, 10297-018),
SuperScript IV Reverse Transcriptase
(
Invitrogen
,
18090050
), and
(28)
Collagen and Microvascularization in Placentas From Young and Older Mares
Frontiers in Veterinary Science
January 4, 2022
Ana Catarina Neto da Silva et al.
Promega
®, Madison, Wisconsin, USA) from 1 μg total RNA in a 20 μL reaction volume using oligonucleotides (
C1101
,
Promega
®, Madison, Wisconsin, USA) and an RNase inhibitor. The obtained cDNA was combined with Power
(29)
A deep learning approach to identify gene targets of a therapeutic for human splicing disorders
Nature Communications
December 1, 2021
Dadi Gao et al.
transcription was performed using 0.5–1 µg of total RNA,
Random Primers
(
C1181
,
Promega
),
Oligo(dT)15 Primer
(
C1101
,
Promega
), and
Superscript III reverse transcriptase
(
18080093
,
ThermoFisher Scientific
) according to
(30)
Triamcinolone acetonide modulates TGF‑β2‑induced angiogenic and tissue‑remodeling effects in cultured human retinal pigment epithelial cells
Molecular medicine reports
November 1, 2021
Hsiao CC, Chang YC, Hsiao YT, Chen PH, Hsieh MC, Wu WC, Kao YH.
avian myeloblastosis virus
reverse transcriptase (cat. no.
M5108
;
Promega Corporation
) and oligo-dT primers (cat. no.
C1101
;
Promega Corporation
) in the presence of a
ribonuclease inhibitor
(RNasin; cat. no.
N2111
;
Promega Corporation
).
(31)
The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine Endometrium
Life (Basel)
October 19, 2021
Ana Amaral, Carina Fernandes, Anna Szóstek-Mioduchowska, Karolina Lukasik, Maria Rosa Rebordão, Pedro Pinto-Bravo, Dariusz Jan Skarzynski, Graça Ferreira-Dias
Promega
, Madison, WI, USA) from 1 μg of total RNA in a 20 μL reaction volume using oligo (dT) primer (
C1101
;
Promega
, Madison, WI, USA). The validation of reference ribosomal protein L32 (RPL32) and target COL1A2
(32)
Functional and Adaptive Significance of Promoter Mutations That Affect Divergent Myocardial Expressions of TRIM72 in Primates
Molecular biology and evolution
June 25, 2021
Feng Y, Xu H, Liu J, Xie N, Gao L, He Y, Yao Y, Lv F, Zhang Y, Lu J,
et al
.
(
Invitrogen
), and 2 μg total RNA was reverse transcribed using MLV reverse transcriptase and oligo-dT (
C1101
,
Promega
). 2 μl cDNA and PrimeSTAR HS premix was used for reverse transcript
PCR
, and 10 μl of
PCR
products
(33)
Th2 Cytokines Increase the Expression of Fibroblast Growth Factor 21 in the Liver
Cells
May 24, 2021
Kang SG, Lee SE, Choi MJ, Chang JY, Kim JT, Zhang BY, Kang YE, Lee JH, Yi HS, Shong M.
hepatocytes
or
mouse
tissues. cDNA was synthesized from 5 µg of total RNA using oligo(dT)15 primers (
C1101
,
Promega
, Madison, WI, USA) and
M-MLV reverse transcriptase
(28025, Thermo Fisher Scientific).
Quantitative PCR
(34)
Noscapine Acts as a Protease Inhibitor of In Vitro Elastase-Induced Collagen Deposition in Equine Endometrium
International Journal of Molecular Sciences
May 19, 2021
Ana Amaral, Carina Fernandes, Anna Szóstek-Mioduchowska, Maria Rosa Rebordão, Dariusz Jan Skarzynski, Graça Ferreira-Dias
undertaken using reverse transcriptase enzyme (
M5313
;
Promega
; Madison, WI, USA) and oligo (dT) primer (
C1101
;
Promega
; Madison, WI, USA) from 1000 ng total RNA in a 20 µL reaction volume. The validation of the
(35)
WNT3A‐loaded exosomes enable cartilage repair
Journal of Extracellular Vesicles
May 19, 2021
Bethan L. Thomas et al.
TRIzolTM reagent (
Invitrogen
15596026
), cDNA was then synthesized using oligo(dt) primers (
Promega
C1101
) and SuperscriptTM III Reverse transcriptase (
Invitrogen
18080093
).
QPCR
analysis was performed for
(36)
TFG binds LC3C to regulate ULK1 localization and autophagosome formation
The EMBO journal
May 17, 2021
Carinci M, Testa B, Bordi M, Milletti G, Bonora M, Antonucci L, Ferraina C, Carro M, Kumar M, Ceglie D,
et al
.
mini kit (
QIAGEN
,
217004
). One µg RNA was retrotranscribed using M‐MLV enzyme and oligodT (
Promega
,
C1101
).
qPCR
was performed using
SYBR Green PCR Master Mix
Applied Biosystems
(
Thermo Fisher Scientific
,
4309155
)
(37)
The deubiquitylase USP9X controls ribosomal stalling
The Journal of cell biology
March 1, 2021
Clancy A, Heride C, Pinto-Fernández A, Elcocks H, Kallinos A, Kayser-Bricker KJ, Wang W, Smith V, Davis S, Fessler S,
et al
.
with RNasin (Promega; N251S),
PCR nucleotide mix
(
Promega
; U144B) and
oligo (dT) 15 Primer
(
Promega
;
C1101
). Quantitative PCRs were performed in triplicate using primers against
ZNF598
(5′-GCTCATCCAGTCCATCAGGG-3′;
(38)
MiR-CLIP reveals
iso
-miR selective regulation in the miR-124 targetome
Nucleic Acids Research
January 11, 2021
Yuluan Wang, Charlotte Soneson, Anna L Malinowska, Artur Laski, Souvik Ghosh, Alexander Kanitz, Luca F R Gebert, Mark D Robinson, Jonathan Hall
concentration of: 1× RT buffer, 1× dNTP mix (4 mM), 12.5 μM random hexamers (Microsynth), 12.5 μM oligo(dT)15 (
C1101
,
Promega
), 2.5 U multiscribe Reverse transcriptase, 2 U RNAse inhibitor (RNasin,
N2115
,
Promega
) was
(39)
Cryptic variation in RNA-directed DNA-methylation controls lateral root development when auxin signalling is perturbed
Nature Communications
December 1, 2020
Zaigham Shahzad, Ross Eaglesfield, Craig Carr, Anna Amtmann
M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant
(
Promega
, cat#
M3681
) and
Oligo(dT)15 Primer
(
Promega
, cat#
C1101
) in a final volume of 25 μL, according to the manufacturer’s instructions. 25 ng of first strand cDNA
(40)
Effects of dietary polyunsaturated fatty acid sources on expression of lipid-related genes in bovine milk somatic cells
Scientific Reports
December 1, 2020
Einar Vargas-Bello-Pérez, Nathaly Cancino-Padilla, Carolina Geldsetzer-Mendoza, María Sol Morales, Heidi Leskinen, Philip C. Garnsworthy, Juan J. Loor, Jaime Romero
(
Promega
, Wisconsin, USA). Total RNA was combined with 0.5 µg/reaction
oligo (dT)15 primer
(Cat. No.
C1101
;
Promega
, Madison, USA) to a final volume of 5 µL and was incubated at 70 °C for 5 min. Next, 15 µL
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Oligo(dT)15 Primer from Promega
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