M220 Focused-ultrasonicator from Covaris LLC

Product Specs
ItemM220 Focused-ultrasonicator
CompanyCovaris LLC
Price Get Pricing Get Quote for this Product
Catalog Number500295
ApplicationsDNA shearing 150 bp to 5 kb
MethodAcoustic Energy (Ultrasonic)
Dimensions(WxDxH) 33 x 38.7 x 27.3 centimeters
Sample VesslesSnap-Cap microTUBE/Screw-Cap microTUBE/Clear miniTUBE/Blue miniTUBE/Red miniTUBE
Regulatory StatusCE, ETL Mark (for Product Safety), WEEE
TypeFocused-ultrasonicator
Capacity50 to 130 uL
Motor75 W
QuantityEA
RangeDNA shearing 150 bp to 5 kb
FormatmicroTUBE, microTUBE-50, milliTUBE 1 ml and miniTUBE
Electrical Requirements75W PIP (Peak Incident Power)
TemperatureAmbient temperature: 19°C to 25°C (66°F to 77°C) Relative humidity: 30% to 70%
Volume50 to 130 uL
ComponentsmicroTUBE:microTUBE-50:milliTUBE 1 ml:miniTUBE

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Covaris LLC

14 Gill St, Unit H

Woburn, Massachusetts 01801-1721

United States

Phone: +1 781-932-3959
Fax: +1 781-932-8705

Email: info@covarisinc.com

Company Profile

Website: www.covaris.com

(1) Supplementation with high-GABA-producing Lactobacillus plantarum L5 ameliorates essential tremor triggered by decreased gut bacteria-derived GABATransl NeurodegenerDecember 13, 2023Hao-Jie Zhong, Si-Qi Wang, Ruo-Xin Zhang, Yu-Pei Zhuang, Longyan Li, Shuo-Zhao Yi, Ying Li, Lei Wu, Yu Ding, Jumei Zhang, et al.agarose gel electrophoresis. The DNA samples were then fragmented to a mean size of ~ 400 bp using an M220 Focused-ultrasonicator™ (Covaris, Brighton, UK). A sequencing library was prepared using the NEXTFLEX® Rapid DNA-Seq Kit (PerkinElmer

(2) Genomic profiling of ovarian clear cell carcinoma in Chinese patients reveals potential prognostic biomarkers for survivalAnnals of MedicineDecember 12, 2023Shuang Ye, Shuling Zhou, Yutuan Wu, Xuan Pei, Wei Jiang, Wanling Shi, Wentao Yang, Xiaoyan Zhou, Boer Shan, Huijuan YangQubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA) were used to measure DNA concentration. The M220 Focused-ultrasonicator (Covaris, Woburn, MA) was used to shear DNA, followed by end repair, phosphorylation and adaptor ligation.

(3) Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxivNovember 30, 2023Tanner Wiegand, Florian T. Hoffmann, Matt W.G. Walker, Stephen Tang, Egill Richard, Hoang C. Le, Chance Meers, Samuel H. Sternbergconjugated to ANTI-FLAG M2 antibodies produced in mouse (Sigma-Aldrich). Samples were sonicated on a M220 Focused-ultrasonicator (Covaris) with the following SonoLab 7.2 settings: minimum temperature, 4 °C; set point, 6 °C; maximum

(4) Are rabid raccoons (Procyon lotor) ready for the rapture? Determining the geographic origin of rabies virus‐infected raccoons using RADcapture and microhaplotypesEvolutionary ApplicationsNovember 20, 2023Matthew W. Hopken, Antoinette J. Piaggio, Zaid Abdo, Richard B. Chipman, Clara P. Mankowski, Kathleen M. Nelson, Mikaela Samsel Hilton, Christine Thurber, Mirian T. N. Tsuchiya, Jesús E. Maldonado, et al.resuspended in 250 μL of low TE (ThermoFisher 12090015) and sheared in a M220 Focused‐Ultrasonicator (Covaris 500295). We used the default DNA_0300_bp_microTUBE_130ul_HolderXTU method and evaluated shearing efficiency

(5) Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2–ERK depending on downregulation of proteasome-related genesJournal of Biological ChemistryNovember 1, 2023Xinzhi Li, Chunhong Liu, Zhimin Zhang, Xueying Li, Zhicheng Yao, Yanbin Dong, Xin Wang, Zheng Chen1% paraformaldehyde was used to fix the livers. The nuclei were extracted from livers and sonicated (M220 Focused-ultrasonicator; Covaris) to break genomic DNA into 500- to 1000-bp fragments using a chromatin shearing kit (520127;

(6) A Comprehensive Genetic Study of Microtubule-Associated Gene Clusters for Male Infertility in a Taiwanese CohortInternational Journal of Molecular SciencesOctober 19, 2023Chying-Chyuan Chan, Te-Hsin Yen, Hao-Chen Tseng, Brang Mai, Pin-Kuan Ho, Jian-Liang Chou, Gwo-Jang Wu, Yu-Chuan Huang(Illumina, San Diego, CA, USA) guidelines, and fragmented 100 ng of genomic DNA into 150 bp inserts by the M220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). Briefly, fragment gDNA is ligated with adapters and enriched by PCR. After

(7) Immune checkpoint inhibitor‐related molecular markers predict prognosis in extrahepatic cholangiocarcinomaCancer MedicineOctober 10, 2023Bao Jin, Yuxin Wang, Baoluhe Zhang, Haifeng Xu, Xin Lu, Xinting Sang, Wenze Wang, Yilei Mao, Pengxiao Chen, Shun Wang, et al.from formalin‐fixed paraffin‐embedded (FFPE) samples and then was broke into ~250 bp fragments through M220 Focused‐ultrasonicator (Covaris). SureSelect Human All Exon V6 Kit (Agilent Technologies) was used for whole genome library

(8) Relationship between the Rod complex and peptidoglycan structure in Escherichia coliMicrobiologyOpenOctober 9, 2023Risa Ago, Yuhei O. Tahara, Honoka Yamaguchi, Motoya Saito, Wakana Ito, Kaito Yamasaki, Taishi Kasai, Sho Okamoto, Taiki Chikada, Taku Oshima, et al.the Wizard Genomic DNA Purification Kit (Promega). One microgram of genomic DNA was sheared using an M220 focused ultrasonicator (Covaris) to obtain peak fragment lengths of 500–600 bp. Next, the NEBNext Ultra DNA Library Preparation

(9) Possible Role of Mitochondrial Transfer RNA Gene 5816 A > G Genetic Polymorphism (m.5816A > G) in a 3-Year-Old Child with Dystonia: Report of a CaseGlobal Medical GeneticsSeptember 29, 2023Sumei Wang, Minglu Liang, Jiehui Ma, Sheng Huang, Lili Fan, Feng Zhu, Dan Sunby agarose gel analysis and quantity was measured by NanoDrop2000 and Qubit3.0. DNA is sheared with M220 Focused-ultrasonicator (Covaris, Woburn, Massachusetts, United States). DNA target regions were captured by hybridizing the

(10) Adaptive therapy achieves long-term control of chemotherapy resistance in high grade ovarian cancerbioRxivSeptember 26, 2023Helen Hockings, Eszter Lakatos, Weini Huang, Maximilian Mossner, Mohammed Ateeb Khan, Stephen Metcalf, Francesco Nicolini, Kane Smith, Ann-Marie Baker, Trevor A. Graham, et al.islands of malignant cells. Genomic and tissue DNA was sonicated into fragments of 150 base pairs using a M220™ Focused-ultrasonicator (Covaris) and library preparation performed using NEBNext® FFPE DNA Repair Mix (New England Biolabs®

(11) Lineage tracing for multiple lung cancer by spatiotemporal heterogeneity using a multi-omics analysis method integrating genomic, transcriptomic, and immune-related featuresFrontiers in OncologySeptember 14, 2023Yijun Song, Jiebai Zhou, Xiaotian Zhao, Yong Zhang, Xiaobo Xu, Donghui Zhang, Jiaohui Pang, Hairong Bao, Yuan Ji, Mengna Zhan, et al.Technologies, Santa Clara, CA, USA). Genomic DNA was sheared into approximately 250-bp fragments by M220 Focused-ultrasonicator (Covaris, Woburn, MA, USA). A whole-genome library was prepared using the KAPA Hyper Prep Kit (KAPA

(12) Analysis of Methylome, Transcriptome, and Lipid Metabolites to Understand the Molecular Abnormalities in Polycystic Ovary SyndromeDiabetes, Metabolic Syndrome and ObesitySeptember 11, 2023Fei Zhang, Yicen Ding, Bohan Zhang, Mengju He, Zhijiang Wang, Chunbo Lu, Yani Kangto compare the methylation profiled regions. Briefly, 1 μg genomic DNA per sample was sheared using M220 Focused-ultrasonicator (Covaris, Woburn, MA, USA), and the sheared DNA with methylated regions was affinity-enriched using

(13) Prevalence and spectrum of cancer predisposition germline mutations in young patients with the common late‐onset cancersCancer MedicineSeptember 1, 2023Shaoyu Hao, Ximeng Zhao, Yue Fan, Zhengchuang Liu, Xiang Zhang, Wei Li, Hongling Yuan, Jie Zhang, Yanxiang Zhang, Tonghui Ma, et al.sequencing were described in a previous study. 37 Briefly, gDNA were sheared in ~200 bp fragments by M220 Focused‐ultrasonicator (Covaris). DNA libraries were constructed with KAPA HTP Library Preparation Kit (KAPA Biosystems), the

(14) Single-cell RNA sequencing to detect age-associated genes that identify senescent cells in the liver of aged miceScientific ReportsAugust 30, 2023Yuta Doshida, Shinichi Hashimoto, Sadahiro Iwabuchi, Yuka Takino, Toshiyuki Ishiwata, Toshiro Aigaki, Akihito Ishigamibeads, and cDNA of 500–2000 bp size was extracted, following the fragmentation to 300–350 bp using the M220 Focused-ultrasonicator (Covaris Inc., Woburn, MS, USA). A sequence library was produced following the instructions of the Illumina

(15) Clonal dynamics and Stereo-seq resolve origin and phenotypic plasticity of adenosquamous carcinomaNPJ Precis OncolAugust 26, 2023Ruiying Zhao, Yunhua Xu, Yedan Chen, Jiajun Zhang, Fei Teng, Sha Liao, Shengnan Chen, Qian Wu, Chan Xiang, Jiaohui Pang, et al.FFPE Tissue Kit (Qiagen) and DNeasy Blood and tissue kit (Qiagen), respectively, and fragmented by M220 Focused-ultrasonicator (Covaris) into ~250 bp. A whole-genome library was prepared using KAPA Hyper Prep Kit (KAPA Biosystems).

(16) Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in miceJHEP reports : innovation in hepatologyAugust 1, 2023Liu C, Li X, Gao M, Dong Y, Chen Z.perfusion with 1% paraformaldehyde. The nuclei were isolated from livers and subjected to sonication (M220 Focused-ultrasonicator, Covaris, Massachusetts, USA) to break genomic DNA into 500- to 1,000-bp fragments using a chromatin

(17) Epitype-inducing temperatures drive DNA methylation changes during somatic embryogenesis in the long-lived gymnosperm Norway spruceFrontiers in Plant ScienceJuly 20, 2023Marcos Viejo, Torstein Tengs, Igor Yakovlev, Hugh Cross, Paal Krokene, Jorunn E. Olsen, Carl Gunnar Fossdala Nanodrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, US). After shearing DNA using a M220 Focused-ultrasonicator (Covaris, Inc., Woburn, MA, US), DNA fragments were bisulfite-converted using the EZ DNA Methylation-Gold

(18) Metagenomics reveals the abundance and accumulation trend of antibiotic resistance gene profile under long-term no tillage in a rainfed agroecosystemFrontiers in MicrobiologyJuly 20, 2023Weiyan Wang, Pengfei Shen, Zhiqiang Lu, Fei Mo, Yuncheng Liao, Xiaoxia Wenstage (two tillage practices × with three replicates) and selected for shotgun metagenomic sequencing. M220 Focused-ultrasonicator (Covaris M220, Gene Company Limited, Hong Kong, China) was used to break the microbial genomic DNA into

(19) Targeted metagenomics using bait-capture to detect antibiotic resistance genes in retail meat and seafoodFrontiers in MicrobiologyJuly 13, 2023Annika Flint, Ashley Cooper, Mary Rao, Kelly Weedmark, Catherine Carrillo, Sandeep Tamber(unbaited) Samples were fragmented using 2.5 μg of input DNA in 130 μL microTUBE snap-cap tubes using a M220 Focused Ultrasonicator (Covaris) to an approximate average fragment length of 700 bp according to manufacturer instructions.

(20) Haplotype-resolved chromosomal-level assembly of wasabi (Eutrema japonicum) genomeScientific DataJuly 11, 2023Hiroyuki Tanaka, Tatsuki Hori, Shohei Yamamoto, Atsushi Toyoda, Kentaro Yano, Kyoko Yamane, Takehiko Itohwere obtained (Table 1). In addition, genomic DNA was fragmented to an average size of 600 bp using a M220 Focused-ultrasonicator (Covaris Inc., MA, USA). A paired-end library with insert sizes from to 550–650 bp was constructed using

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