Description
- for use in mammalian cell lysis; with protease inhibitors
- components supplied in four vials: 1X lysis buffer, PMSF, protease inhibitor cocktail and sodium orthovanadate
- Combine 10 µl PMSF solution, 10 µl sodium orthovanadate solution and 10-20 µl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.
- Use 3 ml complete RIPA per gram of tissue OR 1 ml complete RIPA per 2.0x107 cells in suspension OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.