gentleMACS Octo Dissociator from Miltenyi Biotec

(1) Suppression of prostate cancer and amelioration of the immunosuppressive tumor microenvironment through selective immunoproteasome inhibitionOncoImmunologyDecember 31, 2023Julia Koerner, Dennis Horvath, Franziska Oliveri, Jun Li, Michael BaslerFlow cytometry Single-cell suspensions from tumors were generated using the mouse tumor dissociation kit with the gentleMACS Octo Dissociator according to the manufacturer’s protocol (Miltenyi Biotec). Cells were labeled with fluorochrome-conjugated

(2) Thrombocyte-derived Dickkopf1 promotes macrophage polarization in the Bleomycin-induced lung injury modelFront ImmunolDecember 15, 2023Eun-Ah Sung, Min Hee Park, SuJeong Song, Hasan Alanya, Octavian Henegariu, Jinze Liu, E Zeynep Erson-Omay, Patricia J. Sime, Wook-Jin Chae(v/v) FBS (100-106, Gemini Bio Products), and 40 μg/ml DNase I (10104159001, Roche) in PBS using the gentleMACS Octo Dissociator (Miltenyi Biotec). After blocking the Fc Receptor, cells were stained with Zombie Aqua Fixable Viability

(3) Leptin antagonism attenuates hypertension and renal injury in an experimental model of autoimmune diseaseClinical ScienceDecember 14, 2023William J. Kalusche, Clinton T. Kalusche, Clinton T Taylorhomogenized using the Spleen Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and the GentleMACS Octo Dissociator (Miltenyi Biotec), according to the manufacturer’s instructions. Kidney For isolation of renal immune

(4) Morphologic, phenotypic, and genotypic similarities between primary tumors and corresponding 3D cell cultures grown in a repeatable system-preliminary resultsBMC Vet ResDecember 9, 2023Luisa Vera Muscatello, Stella Frabetti, Giancarlo Avallone, Francesca Gobbo, Arianna Pasquini, Giulia D'Annunzio, Luciano Pisoni, Laura Marconato, Rossella Terragni, Dario De Biase, et al.specimens were processed to obtain single cells using the Tumor Dissociation Kit (Miltenyi Biotec) with GentleMACS Octo Dissociators instrument with heaters (Miltenyi Biotec). The single cells obtained from the dissociation

(5) The coenzyme A precursor pantethine enhances antitumor immunity in sarcomaLife Science AllianceDecember 1, 2023Richard Miallot, Virginie Millet, Anais Roger, Romain Fenouil, Catherine Tardivel, Jean-Charles Martin, Laetitia Shintu, Paul Berchard, Juliane Sousa Lanza, Bernard Malissen, et al.experimental unit referred to as n. Tumors were mechanically and enzymatically digested using Miltenyi Biotech GentleMACS Octo Dissociator technology. The samples were filtered through a 70-μm cell strainer (Becton Dickinson) and subjected

(6) Temporal genomic analysis of melanoma rejection identifies regulators of tumor immune evasionbioRxivDecember 1, 2023Sapir Cohen Cohen Shvefel, Joy A. Pai, Yingying Cao, Lipika R. Pal, Ronen Levy, Winnie Yao, Kuoyuan Cheng, Marie Zemanek, Osnat Bartok, Chen Weller, et al.into small pieces. The minced tumors were dissociated using the Miltenyi tumor dissociation kit and a gentleMacs octo dissociator with the soft/medium tumor program, then subjected to red blood cell (RBC) lysis. The resulting cell

(7) Modulation of recovery from neonatal hyperoxic lung injury by sex as a biological variableRedox BiologyDecember 1, 2023Abiud Cantu, Manuel Cantu Gutierrez, Xiaoyu Dong, Connor Leek, Montserrat Anguera, Krithika Lingappancontinuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-μM filter, washed,

(8) A novel anti-galectin-9 immunotherapy limits the early progression of pancreatic neoplastic lesions in transgenic miceFrontiers in ImmunologyNovember 30, 2023Alexandre Quilbe, Rami Mustapha, Belinda Duchêne, Abhishek Kumar, Elisabeth Werkmeister, Emmanuelle Leteurtre, Olivier Moralès, Nicolas Jonckheere, Isabelle Van Seuningen, Nadira DelhemTumour Dissociation Kit and gentleMACS™ M Tubes (Miltenyi, Gladbach, Germany) for the pancreas using the gentleMACS™ Octo Dissociator (Miltenyi, Gladbach, Germany) following the manufacturer’s instructions. The final suspension was passed

(9) Differential in vivo labeling with barcoded antibodies allows for simultaneous transcriptomic profiling of airway, lung tissue and intravascular immune cellsFrontiers in ImmunologyNovember 29, 2023Barbara C. Mindt, John Kim, Troy Warren, Yang Song, Antonio DiGiandomenicoSystem, 0.22 µm pore size (Corning, cat# 431096)GentleMACS™ C tubes (Miltenyi Biotec, cat# 130-093-237)GentleMACS™ Octo Dissociator (Miltenyi Biotec, cat# 130-096-427)50 mL Centrifuge tubes (VWR, cat# 89039-662)70 μM cell strainers

(10) Minimal effective dose of bedaquiline administered orally and activity of a long acting formulation of bedaquiline in the murine model of leprosyPLOS Neglected Tropical DiseasesNovember 27, 2023Aurélie Chauffour, Nacer Lounis, Koen Andries, Vincent Jarlier, Nicolas Veziris, Alexandra Aubrysuspension [25]. Briefly, the tissue from the footpads was aseptically removed and then grinded by using a GentleMacs Octo Dissociator (Miltenyi) under a volume of 2 ml of Hanks’ balanced salt solution. Ten μl of the prepared suspension

(11) GITR and TIGIT immunotherapy provokes divergent multicellular responses in the tumor microenvironment of gastrointestinal cancersGenome MedicineNovember 26, 2023Anuja Sathe, Carlos Ayala, Xiangqi Bai, Susan M. Grimes, Byrne Lee, Cindy Kin, Andrew Shelton, George Poultsides, Hanlee P. Jidissociation Tissue dissociation was conducted using a combination of enzymatic and mechanical dissociation using a gentleMACS Octo Dissociator (Miltenyi Biotec) as described previously [18]. Cells were cryofrozen using 10% DMSO in 90% FBS (Thermo

(12) Autophagy drives the conversion of developmental neural stem cells to the adult quiescent stateNature CommunicationsNovember 24, 2023Isabel Calatayud-Baselga, Lucía Casares-Crespo, Carmina Franch-Ibáñez, José Guijarro-Nuez, Pascual Sanz, Helena MiraNeural Tissue Dissociation Kit (Miltenyi, 130-092-628) with gentle MACS Octo Dissociator (Miltenyi, 130-095-937). GLAST-positive cells were isolated by MACS. Cells were labeled with Anti-GLAST (ACSA1) microBead Kit

(13) ApoE isoform does not influence skeletal muscle regeneration in adult miceFrontiers in PhysiologyNovember 22, 2023Benjamin I. Burke, Jensen Goh, Fatmah A. Albathi, Taylor R. Valentino, Georgia L. Nolt, Jai K. Joshi, Cory M. Dungan, Lance A. Johnson, Yuan Wen, Ahmed Ismaeel, et al.isolated from the hindlimb musculature of ApoE2, E3, and E4 mice. Tissue was homogenized using the gentleMACS Octo Dissociator and Skeletal Muscle Dissociation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) according to manufacturer’s

(14) YAP localization mediates mechanical adaptation of human cancer cells during extravasation in vivobioRxivNovember 21, 2023Woong Young So, Claudia S. Wong, Udochi F. Azubuike, Colin D. Paul, Paniz Rezvan Sangsari, Patricia B. Gordon, Hyeyeon Gong, Tapan K. Maity, Perry Lim, Zhilin Yang, et al.samples were shipped overnight in DMEM on ice. The samples were dissociated using the Miltenyi Biotec gentleMACS Octo Dissociator with heater and the Miltenyi Biotec tumor dissociation kit, human using the manufacturer’s protocol.

(15) YAP-Driven Malignant Reprogramming of Epithelial Stem Cells at Single Cell ResolutionResearch SquareNovember 16, 2023J. Silvio Gutkind, Farhoud Faraji, Sydney Ramirez, Lauren Clubb, Kuniaki Sato, Paola Anguiano Quiroz, William Galloway, Zbigniew Mikulski, Thomas Hoang, Kate Medetgul-Ernar, et al.(Thermo) and subjected to mechanical dissociation in the gentleMACS dissociator C tubes (Miltenyi #130–095-937) for 12 minutes at 37°C, followed by inactivation of trypsin and filtration. Primary epithelial cell

(16) Antitumor activity of AZD0754, a dnTGFβRII-armored, STEAP2-targeted CAR-T cell therapy, in prostate cancerJournal of Clinical InvestigationNovember 15, 2023Peter Zanvit, Dewald van Dyk, Christine Fazenbaker, Kelly McGlinchey, Weichuan Luo, Jessica M. Pezold, John Meekin, Chien-ying Chang, Rosa A. Carrasco, Shannon Breen, et al.DNase I (Sigma-Aldrich), and hyaluronidase (Sigma-Aldrich). Single-cell suspensions were generated using GentleMACS Octo dissociator (Miltenyi Biotec). Dead cells were excluded by staining with a Live/Dead Fluorescent Blue Dead Cell Stain

(17) RNA m6A methylation modulates airway inflammation in allergic asthma via PTX3-dependent macrophage homeostasisNature CommunicationsNovember 13, 2023Xiao Han, Lijuan Liu, Saihua Huang, Wenfeng Xiao, Yajing Gao, Weitao Zhou, Caiyan Zhang, Hongmei Zheng, Lan Yang, Xueru Xie, et al.(Miltenyi Biotech). Tissues in C Tubes were subjected to the run program 37 °C_m_lung for 30 min on the gentleMACS Octo Dissociator. Cell suspensions were passed through a 70 µm MACS SmartStrainer (Miltenyi Biotech), followed by centrifugation

(18) Defective LAT signalosome pathology in mice mimics human IgG4-related disease at single-cell levelJ Exp MedNovember 6, 2023Anais Joachim, Rudy Aussel, Léna Gélard, Fanghui Zhang, Daiki Mori, Claude Grégoire, Sergio Villazala Merino, Mauro Gaya, Yinming Liang, Marie Malissen, et al.collagenase II (Worthington). Digestion and dissociation were conducted at 37°C in C tubes using the GentleMACS Octo Dissociator (Miltenyi Biotec) according to the manufacturer’s protocol. Cells from spleen and lymph nodes were prepared

(19) Ex vivo to in vivo model of malignant peripheral nerve sheath tumors for precision oncologyNeuro-OncologyNovember 2, 2023Alex T Larsson, Himanshi Bhatia, Ana Calizo, Kai Pollard, Xiaochun Zhang, Eric Conniff, Justin F Tibbitts, Elizabeth Rono, Katherine Cummins, Sara H Osum, et al.digested using the human Tumor Dissociation Kit (130-095-929, Miltenyi Biotec) in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec). At room temperature, the digested PDX was filtered through a 70 µm cell strainer

(20) ATM-Inhibitor AZD1390 Is a Radiosensitizer for Breast Cancer CNS MetastasisClinical Cancer ResearchNovember 1, 2023Ben Yi Tew, Alex J. Kalfa, Zeyi Yang, Kyle M. Hurth, Thomas Simon, Eric Abnoosian, Stephen T. Durant, Petra Hamerlik, Bodour Salhiatumor was dissociated into single cells with the human tumor dissociation kit (Miltenyi) using the gentleMACS Octo Dissociator (Miltenyi) according to the manufacturer's instructions. For flank studies, tumor cells were resuspended

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2/12/2020Good Instrument with a Lot of Features Jackie LeeThis instrument was used for blending fresh tissues instead of doing so by hand.

gentleMACS Octo Dissociator

gentleMACS Octo Dissociator from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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gentleMACS Octo Dissociator from Miltenyi Biotec

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