Total Exosome Isolation Reagent (from cell culture media) from Thermo Fisher Scientific

Product Specs
ItemTotal Exosome Isolation Reagent (from cell culture media)
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number4478359
Brand/TrademarkInvitrogen™
TypeTotal Exosome Isolation Reagent
Sample TypeCell Culture Media

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) A Method for Using Cell-Penetrating Peptides for Loading Plasmid DNA into Secreted Extracellular VesiclesBiomoleculesDecember 6, 2023Jekaterina Nebogatova, Heleri Heike Härk, Anett Puskar, Ly Porosk, Paolo Guazzi, Moataz Dowaidar, Ülo Langel, Kaido Kurrikoffwith the total exosome isolation reagent (from cell culture media) (Invitrogen, Walth, MA, USA, cat.nr.4478359). The pDNA used in experiments with immuno-stained EVs was labeled with a Label IT® Nucleic Acid Labeling

(2) O-GlcNAcylation is essential for therapeutic mitochondrial transplantationCommunications MedicineNovember 25, 2023Ji Hyun Park, Masayoshi Tanaka, Takafumi Nakano, Ester Licastro, Yoshihiko Nakamura, Wenlu Li, Elga Esposito, Emiri T. Mandeville, Sherry Hsiang-Yi Chou, MingMing Ning, et al.lysis/neutralization followed by isopropanol precipitation from conditioned media (Thermo Fisher Scientific, 4478359). Relative levels of mtDNA to genomic DNA were determined by amplifying MT-ND1 gene (Applied Biosystems,

(3) ALS-linked SOD1 mutations impair mitochondrial-derived vesicle formation and accelerate agingRedox BiologyNovember 24, 2023Ying Guo, Teng Guan, Qiang Yu, Nitesh Sanghai, Kashfia Shafiq, Meiyu Li, Xin Jiao, Donghui Na, Guohui Zhang, Jiming Kongwas subsequently filtered with 0.22 mm filters. EVs were isolated by using an exosome isolation kit (4478359, Invitrogen) as previously described [37]. In brief, culture media were harvested and centrifuged at

(4) First insight into extracellular vesicle-miRNA characterization in a sheep in vitro model of inflammationFrontiers in Veterinary ScienceNovember 22, 2023Maria Giovanna Ciliberti, Antonella Santillo, Agostino Sevi, Marzia Albenzio, Vincenzo De Leo, Chiara Ingrosso, Lucia Catucci, Mariangela Caroprese−20°C for further analysis. The REA method was performed according to the manufacturer’s instruction of Total Exosome Isolation Reagent (from cell culture media, Thermo Fisher, Waltham, United States), and the isolation ratio was based on binding the water molecule

(5) Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCsNature CommunicationsNovember 22, 2023Ye He, Shujie Yang, Pengzhan Liu, Ke Li, Ke Jin, Ryan Becker, Jinxin Zhang, Chuanchuan Lin, Jianping Xia, Zhehan Ma, et al.and the total number of exosomes was obtained using a Total Exosome Isolation Reagent (Invitrogen, 4478359). Briefly, the supernatant was centrifuged at 2000 × g for 30 min at 4 °C to remove cells and debris.

(6) Hypoxia-regulated exosomes mediate M2 macrophage polarization and promote metastasis in chondrosarcomaAging (Albany NY)November 21, 2023Sheng-Mou Hou, Chih-Yang Lin, Yi-Chin Fong, Chih-Hsin Tangfrom the cell culture using the total exosome isolation reagent (from cell culture media) (Catalog No: 4478359) obtained from Life Technologies. The steps are as follows: Transfer the required volume of cell-free

(7) A Single-Step Immunocapture Assay to Quantify HCC Exosomes Using the Highly Sensitive Fluorescence Nanoparticle-Tracking AnalysisJournal of Hepatocellular CarcinomaNovember 2, 2023Ali Riza Koksal, Nergiz Ekmen, Yucel Aydin, Kelley Nunez, Tyler Sandow, Molly Delk, Martin Moehlen, Paul Thevenot, Ari Cohen, Srikanta Dashsupernatants were transferred to a new tube and mixed with 0.5 volumes of total EV isolation reagent (4478359) from Invitrogen Carlsbad, CA. Tubes were incubated at 4°C overnight. The following day samples were

(8) Palmitate and glucose increase amyloid precursor protein in extracellular vesicles: Missing link between metabolic syndrome and Alzheimer's diseaseJournal of Extracellular VesiclesNovember 1, 2023Bhumsoo Kim, Yoon‐Tae Kang, Faye E. Mendelson, John M. Hayes, Masha G. Savelieff, Sunitha Nagrath, Eva L. Feldmanneurons EVs were isolated from cell culture media using Total Exosome Isolation Reagent (TEIR, catalog # 4478359, Invitrogen, Thermo Fisher Scientific) as previously described (Helwa et al., 2017), followed by ultracentrifugation.

(9) Fe65-engineered neuronal exosomes encapsulating corynoxine-B ameliorate cognition and pathology of Alzheimer’s diseaseSignal Transduction and Targeted TherapyOctober 23, 2023Ashok Iyaswamy, Abhimanyu Thakur, Xin-Jie Guan, Senthilkumar Krishnamoorthi, Tsz Yan Fung, Kejia Lu, Isha Gaurav, Zhijun Yang, Cheng-Fu Su, Kwok-Fai Lau, et al.FluorSave reagent (Cat# 345789, Calbiochem). Total Exosome Isolation (TEI) reagent (ThermoFisher Scientific, Cat# 4478359). The details of antibodies used in this study are listed in Supplementary Table S1. Animals Animal

(10) Comparison Study of Small Extracellular Vesicle Isolation Methods for Profiling Protein Biomarkers in Breast Cancer Liquid BiopsiesInternational Journal of Molecular SciencesOctober 23, 2023Yujin Lee, Jie Ni, Valerie C. Wasinger, Peter Graham, Yong LiIsolation To isolate sEVs from both the cell culture media and plasma, the TEI (Invitrogen (Waltham, MA, USA), 4478359 for the cell culture media and 4484450 for the plasma), UC, and a combination of UC and TEI (UCT) were

(11) Clonal and Scalable Endothelial Progenitor Cell Lines from Human Pluripotent Stem CellsBiomedicinesOctober 13, 2023Jieun Lee, Hal Sternberg, Paola A. Bignone, James Murai, Nafees N. Malik, Michael D. West, Dana Laroccaculture medium using the precipitation method following the manufacturer’s instructions (Invitrogen, Cat#4478359, Waltham, MA, USA). Total exosome particles were counted using nanoparticle tracking analysis (NTA). Tube

(12) Exosomes derived from mesenchymal stem cells primed with disease-condition-serum improved therapeutic efficacy in a mouse rheumatoid arthritis model via enhanced TGF-β1 productionStem Cell Research & TherapyOctober 4, 2023Eun Wha Choi, I.-Rang Lim, Ji Hong Park, Jiwoo Song, Bongkum Choi, Sungjoo Kimserum (Exo-RA), or non-RA serum (Exo-non-RA) were isolated using the Total Exosome Isolation Reagent (4478359, Invitrogen, Waltham, Massachusetts, USA) according to the manufacturer’s instructions. The total protein

(13) Engineered exosomes as drug and RNA co-delivery system: new hope for enhanced therapeutics?Frontiers in Bioengineering and BiotechnologySeptember 26, 2023Haorong Chen, Hanbo Yao, Jiaxin Chi, Chaowei Li, Yilin Liu, Jiayi Yang, Jiaqi Yu, Jiajun Wang, Yongdui Ruan, Jiang Pi, et al.healthy donors,miR-15p-3p,0.82,Upregulated,Wei et al. (2020a), Cancer,CRC,"Total Exosome Isolation Kits (4478359 and 4478360) (Invitrogen, NYC, United States)","Serum; 135 CRC patients, 35 BID patients and 45 HCs",hsa-circ-0004771,0.86/0.88,Upregulated,Pan

(14) Dynamic Interplay in Tumor Ecosystems: Communication between Hepatoma Cells and FibroblastsInternational Journal of Molecular SciencesSeptember 12, 2023Gábor Petővári, Gábor Tóth, Lilla Turiák, Anna L. Kiss, Krisztina Pálóczi, Anna Sebestyén, Adrián Pesti, András Kiss, Kornélia Baghy, Katalin Dezső, et al.and proteins present in fetal bovine serum. EVs were isolated using total exosome isolation reagent (#4478359, Thermo Fisher Scientific Inc., Waltham, MA, USA) following the instructions of the manufacturer. In

(15) A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesiclesNature CommunicationsSeptember 11, 2023Kangfu Chen, Bill T. V. Duong, Sharif U. Ahmed, Piriththiv Dhavarasa, Zongjie Wang, Mahmoud Labib, Connor Flynn, Jingya Xu, Yi Y. Zhang, Hansen Wang, et al.microvesicles. The supernatant were filtered with 0.22 µm filter and then mixed with an sEV precipitation kit (4478359, Thermofisher) at a 2:1 volume ratio, incubated overnight, and then centrifuged at 10,000 g for 1 h

(16) Mesenchymal Stem Cell-Derived Exosomal miRNA-222-3p Increases Th1/Th2 Ratio and Promotes Apoptosis of Acute Myeloid Leukemia CellsAnal Cell Pathol (Amst)August 16, 2023Yuan Yuan, Shengfen Tan, Huanhuan Wang, Junfeng Zhu, Jiajia Li, Pingping Zhang, Meng Wang, Feng Zhangat 4°C. The precipitates containing the exosomes were obtained and resuspended in PS buffer (500 μl, 4478359, Invitrogen). The exosomes were stored at −80°C for further analysis. 2.3. Transmission Electron Microscopy Transmission electron microscopy

(17) Discovery and Targeting of a Noncanonical Mechanism of Sarcoma Resistance to ADI-PEG20 Mediated by the MicroenvironmentClinical Cancer ResearchAugust 15, 2023Leonard C. Rogers, Jeff C. Kremer, Caitlyn B. Brashears, Zongtao Lin, Zhixian Hu, Alliny C.S. Bastos, Adriana Baker, Nicole Fettig, Dong Zhou, Kooresh I. Shoghi, et al.105 cells each. The next day, 5 mL of media was added to each plate for 24 hours, then harvested with Total Exosome Isolation Reagent (from cell culture media; Thermo Fisher Scientific) according to the manufacturer's instructions. Isolated EVs were resuspended

(18) Amniotic Membrane-Derived Stromal Cells Release Extracellular Vesicles That Favor Regeneration of Dystrophic Skeletal MusclesInt J Mol SciAugust 5, 2023Martina Sandonà, Federica Esposito, Anna Cargnoni, Antonietta Silini, Pietro Romele, Ornella Parolini, Valentina Sacconewere isolated from CM hAMSC and in parallel from CM CTR with Total Exosomes Isolation Reagent-TEIR (#4478359, Invitrogen by Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer instructions. 4.2.1.

(19) Vitamin D receptor-deficient keratinocytes-derived exosomal miR-4505 promotes the macrophage polarization towards the M1 phenotypePeerJAugust 4, 2023Wen Sun, Jianqin Chen, Jingting Li, Xiaoguang She, Hu Ma, Shali Wang, Jing Liu, Yuan Yuanfrom HaCaT cells The exosomes were isolated from HaCaT cells using Total Exosome Isolation Reagent (4478359; Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. Briefly, the culture supernatants

(20) Human Keratinocytes and Fibroblasts Co-Cultured on Silk Fibroin Scaffolds Exosomally Overrelease Angiogenic and Growth FactorsCellsJuly 11, 2023Peng Hu, Ubaldo Armato, Giuliano Freddi, Anna Chiarini, Ilaria Dal Pràwhole exosomal fraction isolated using the Total Exosome Isolation Reagent for Cell Culture Media (No. 4478359, Thermo Fisher Scientific), following the manufacturer’s protocol, as previously detailed by Hu et al.

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(1)

5/29/2019Time-Saving Exosome Isolation Reagent from Invitrogen Wei Yang SitTotal Exosome Isolation Reagent enables fast and efficient enrichment of intact exosomes from cell culture media samples.
(1)

4/26/2018Isolating Cell Culture Exosomes Rachel CrosslandThe ThermoFisher Exosome Isolation Reagent can be used to isolate small extracellular vesicles from cell conditioned media. The process is quick and simple and does not require specialist equipment. ... Read Review

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Total Exosome Isolation Reagent (from cell culture media) from Thermo Fisher Scientific

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Total Exosome Isolation Reagent (from cell culture media) from Thermo Fisher Scientific

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