Many detergent varieties are available from several suppliers molecular and biochemical procedures such as protein purification, DNA/RNA isolation, staining and microscopy, electrophoresis, immunoassays, and more.
Tween® 80 also known as polysorbate 80, is a nonionic detergent commonly used in cell lysis for protein and nucleic acid extraction. It is also added to immunoassay buffers such as PBS or TBS. At room temperature, Tween® 80 appears as a clear, viscous amber-colored liquid. It has a molar mass of 1,310 g/mol and is soluble in water and alcohol.