HTRF Microplate Readers

Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. These lab workhorses are used in drug discovery, research, bioassay validation, and biopharmaceutical manufacturing.

Throughput, flexibility, and cost are the top three considerations when choosing a plate reader. In pharmaceutical research, for example, throughput might be the top criterion. Regarding flexibility, a single-mode instrument, such as an absorbance reader for ELISAs, may fit the bill (some can capture a full UV-VIS spectrum from 220 to 1000 nm), but a multimode reader that offers additional capabilities such as fluorescence may offset the additional cost outlay. Integrated software, type of plates accepted, and built-ins like incubators and plate shakers are additional considerations.