Methotrexate ELISA kits are designed for the specific detection of methotrexate in biological samples. Methotrexate is a bioactive compound with pharmacological effects. It has the chemical formula C20H22N8O5. Methotrexate is an inhibitor of tetrahydrofolate dehydrogenase and prevents the formation of tetrahydrofolate, necessary for synthesis of thymidylate, an essential component of DNA. Methotrexate anti-tumor activity is a result of the inhibition of folic acid reductase, leading to inhibition of DNA synthesis and inhibition of cellular replication.
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1 Human Metabolome Database (HMDB) v5.0