Anti-RTCB antibodies are used in the immunodetection of the protein RNA 2',3'-cyclic phosphate and 5'-OH ligase. In humans, the canonical protein has a reported length of 505 amino acid residues and a mass of 55.2 kDa. Its subcellular localization is in the nucleus and cytoplasm. It is notably widely expressed in many tissue types. A member of the RtcB protein family, RTCB is a known catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. Synonyms for this target antigen include DJ149A16.6, FAAP, HSPC117, RNA-splicing ligase RtcB homolog, 3'-phosphate/5'-hydroxy nucleic acid ligase, and C22orf28. RTCB gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of RTCB antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.