Anti-ECSIT antibodies are used in the immunodetection of the protein ECSIT signaling integrator. In humans, the canonical protein has a reported length of 431 amino acid residues and a mass of 49.1 kDa. Its subcellular localization is in the nucleus, mitochondria, and cytoplasm. Up to 4 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the ECSIT protein family, ECSIT is reported to be an adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1 and activation of IRF3/IRF7 pathways. Synonyms for this target antigen include evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, ECSIT homolog, ECSIT signalling integrator, and SITPEC. ECSIT gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish and chimpanzee species. Some ECSIT antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.