Anti-ATP6V1F antibodies are used in the immunodetection of the protein ATPase H+ transporting V1 subunit F. In humans, the canonical protein has a reported length of 119 amino acid residues and a mass of 13.4 kDa. Its subcellular localization is in the cytoplasmic vesicles and membrane. Up to 2 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the V-ATPase F subunit protein family, ATP6V1F is a known subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. Synonyms for this target antigen include VATF, Vma7, V-type proton ATPase subunit F, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F, ATPase, vacuolar, 14 kD, and ATP6S14. ATP6V1F gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish and chimpanzee species. Western Blot is the most common application for the ATP6V1F antibodies listed below. ELISA, Flow Cytometry, Immunofluorescence, and Immunohistochemistry are also common applications.