Anti-HOT antibodies enable researchers to detect and measure the HOT antigen in biological samples. This target is a reported synonym of the ADHFE1 gene, which encodes alcohol dehydrogenase iron containing 1. This protein is known to function in the metabolism of lipids, among other biological roles. The human version of HOT has a canonical amino acid length of 467 residues and a protein mass of 50.3 kilodaltons, although 4 isoforms have been identified. It is reported to be localized in the mitochondria of cells and notably expressed in the liver. HOT is a member of the Iron-containing alcohol dehydrogenase protein family. Indicated applications for HOT antibodies listed below include ELISA, Western Blot, and Immunohistochemistry.