Anti-ROBO2 antibodies are used in the immunodetection of the protein roundabout guidance receptor 2. In humans, the canonical protein has a reported length of 1378 amino acid residues and a mass of 151.2 kDa. Its subcellular localization is in the membrane. Up to 3 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the ROBO protein family, ROBO2 is a known receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.
The ROBO2 marker can be used to identify Macula Densa Cells.* Synonyms for this target antigen include roundabout homolog 2, roundabout, axon guidance receptor, homolog 2, and SAX3. ROBO2 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of ROBO2 antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.
*HuBMAP Human Reference Atlas v1.4