Anti-ROBO1 antibodies are used in the immunodetection of the protein roundabout guidance receptor 1. In humans, the canonical protein has a reported length of 1651 amino acid residues and a mass of 180.9 kDa. Its subcellular localization is in the ER and cell membrane. Up to 6 different isoforms have been reported for this protein. It is found to be widely expressed, with exception of kidney. A member of the ROBO protein family, ROBO1 is a known receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.
The ROBO1 marker can be used to identify Mesangial Cells.* Synonyms for this target antigen include DUTT1, NORS, NYS8, SAX3, roundabout homolog 1, and CPHD8. ROBO1 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of ROBO1 antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. In addition, ELISA is also a common application.
*HuBMAP Human Reference Atlas v1.4