Anti-RFFL antibodies are used in the immunodetection of the protein ring finger and FYVE like domain containing E3 ubiquitin protein ligase. In humans, the canonical protein has a reported length of 363 amino acid residues and a mass of 40.5 kDa. Its subcellular localization is in the cell membrane and cytoplasm. Up to 3 different isoforms have been reported for this protein. It is ubiquitously expressed across many tissue types. RFFL is known to be involved with the apoptotic pathway. Post-translational modifications have been described, including ubiquitination, palmitoylation and protein cleavage. Synonyms for this target antigen include FYVE-RING finger protein SAKURA, RING finger and FYVE-like domain-containing protein 1, RING finger protein 189, RING-type E3 ubiquitin transferase rififylin, and E3 ubiquitin-protein ligase rififylin. RFFL gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. Some RFFL antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.