Anti-DPH3 antibodies are used in the immunodetection of the protein diphthamide biosynthesis 3. In humans, the canonical protein has a reported length of 82 amino acid residues and a mass of 9.2 kDa. Its subcellular localization is in the nucleus and cytoplasm. Up to 2 different isoforms have been reported for this protein. It is reported to be widely expressed with highest levels in small intestine, spleen, thymus, heart, liver and lung. A member of the DPH3 protein family, DPH3 is known to be required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. Synonyms for this target antigen include CSL-type zinc finger-containing protein 2, DPH3 homolog (KTI11, S. cerevisiae), DPH3, KTI11 homolog, DPH3A, KTI11 homolog A, and diphthamide biosynthesis protein 3. DPH3 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. ELISA, Immunocytochemistry, and Immunofluorescence are common applications for DPH3 antibodies.