Anti-HCN4 antibodies are used in the immunodetection of the protein hyperpolarization activated cyclic nucleotide gated potassium channel 4. In humans, the canonical protein has a reported length of 1203 amino acid residues and a mass of 129 kDa. Its subcellular localization is in the cell membrane. It is reported to be highly expressed in thalamus, testis and in the heart, both in ventricle and atrium. A member of the Potassium channel HCN protein family, HCN4 is known to be involved with the transport of ions and potassium ion transport. Post-translational modifications have been described, including glycosylation.
The HCN4 marker can be used to identify Pacemaker Cells and Pacemaker Cardiomyocytes.* Synonyms for this target antigen include EIG18, SSS2, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, hyperpolarization activated cyclic nucleotide-gated cation channel 4, and BRGDA8. HCN4 gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species.
*HuBMAP Human Reference Atlas v1.4